(Linear) (Six-base) MAPSORT of: KIEE6572.seq  Check: 7116  from: 1  to: 369



KIEE6572.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

ApaI G_GGCC'C

Cuts at:      0     133     369 
   Size:        133     236
 
AvaI C'yCGr_G

Cuts at:      0       5     369 
   Size:          5     364
 
BanI G'GyrC_C

Cuts at:      0     307     369 
   Size:        307      62
 
BanII G_rGCy'C

Cuts at:      0      71     133     369 
   Size:         71      62     236

  Fragments arranged by size: 

                 236      71      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     359     369 
   Size:        359      10
 
Bme1580I G_kGCm'C

Cuts at:      0     133     312     369 
   Size:        133     179      57

  Fragments arranged by size: 

                 179     133      57
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25      28     336     369 
   Size:         25       3     308      33

  Fragments arranged by size: 

                 308      33      25       3
 
BseYI C'CCAG_C

Cuts at:      0     126     369 
   Size:        126     243
 
BsiEI CG_ry'CG

Cuts at:      0     318     369 
   Size:        318      51
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     133     312     369 
   Size:         71      62     179      57

  Fragments arranged by size: 

                 179      71      62      57
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     131     369 
   Size:        131     238
 
BsrBI CCG'CTC

Cuts at:      0     363     369 
   Size:        363       6
 
BsrFI r'CCGG_y

Cuts at:      0      78     304     369 
   Size:         78     226      65

  Fragments arranged by size: 

                 226      78      65
 
BstYI r'GATC_y

Cuts at:      0     327     369 
   Size:        327      42
 
BtgI C'CryG_G

Cuts at:      0      44     369 
   Size:         44     325
 
EaeI y'GGCC_r

Cuts at:      0      80     369 
   Size:         80     289
 
FspI TGC'GCA

Cuts at:      0     262     369 
   Size:        262     107
 
FspAI rTGC'GCAy

Cuts at:      0     262     369 
   Size:        262     107
 
HaeII r_GCGC'y

Cuts at:      0      17     369 
   Size:         17     352
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     369 
   Size:        265      32      72

  Fragments arranged by size: 

                 265      72      32
 
HindIII A'AGCT_T

Cuts at:      0     225     369 
   Size:        225     144
 
MspA1I CmG'CkG

Cuts at:      0      46     369 
   Size:         46     323
 
NaeI GCC'GGC

Cuts at:      0      80     369 
   Size:         80     289
 
NgoMIV G'CCGG_C

Cuts at:      0      78     369 
   Size:         78     291
 
NspI r_CATG'y

Cuts at:      0     139     369 
   Size:        139     230
 
PfoI T'CCnGG_A

Cuts at:      0     323     369 
   Size:        323      46
 
PspOMI G'GGCC_C

Cuts at:      0     129     369 
   Size:        129     240
 
SacII CC_GC'GG

Cuts at:      0      47     369 
   Size:         47     322
 
StyI C'CwwG_G

Cuts at:      0      83     369 
   Size:         83     286
 

 Enzymes that do cut:

     ApaI     AvaI     BanI    BanII     BbsI Bme1580I    BseRI    BseYI
    BsiEI Bsp1286I    BspMI    BsrBI    BsrFI    BstYI     BtgI     EaeI
     FspI    FspAI    HaeII    Hin4I  HindIII   MspA1I     NaeI   NgoMIV
     NspI     PfoI   PspOMI    SacII     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI