(Linear) (Six-base) MAPSORT of: KIEE6571.seq  Check: 3605  from: 1  to: 435



KIEE6571.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      18     435 
   Size:         18     417
 
ApoI r'AATT_y

Cuts at:      0     319     340     435 
   Size:        319      21      95

  Fragments arranged by size: 

                 319      95      21
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      98     131     435 
   Size:         98      33     304

  Fragments arranged by size: 

                 304      98      33
 
BamHI G'GATC_C

Cuts at:      0     410     435 
   Size:        410      25
 
BanI G'GyrC_C

Cuts at:      0     243     355     435 
   Size:        243     112      80

  Fragments arranged by size: 

                 243     112      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     128     155     435 
   Size:        128      27     280

  Fragments arranged by size: 

                 280     128      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      61     435 
   Size:         61     374
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     435 
   Size:         31     404
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       3     435 
   Size:          3     432
 
BsrFI r'CCGG_y

Cuts at:      0      43     435 
   Size:         43     392
 
BstYI r'GATC_y

Cuts at:      0     410     435 
   Size:        410      25
 
BtgI C'CryG_G

Cuts at:      0      48     435 
   Size:         48     387
 
EaeI y'GGCC_r

Cuts at:      0      45     168     435 
   Size:         45     123     267

  Fragments arranged by size: 

                 267     123      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     435 
   Size:         15     420
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     270     302     435 
   Size:        270      32     133

  Fragments arranged by size: 

                 270     133      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     435 
   Size:        316     119
 
PpuMI rG'GwC_Cy

Cuts at:      0      15     435 
   Size:         15     420
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     356     388     435 
   Size:        356      32      47

  Fragments arranged by size: 

                 356      47      32
 
PstI C_TGCA'G

Cuts at:      0      35     435 
   Size:         35     400
 
SfcI C'TryA_G

Cuts at:      0      31     435 
   Size:         31     404
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     227     259     435 
   Size:        227      32     176

  Fragments arranged by size: 

                 227     176      32
 

 Enzymes that do cut:

    AlwNI     ApoI     BaeI    BamHI     BanI     BbsI     BsaI     BsgI
    BspMI    BsrFI    BstYI     BtgI     EaeI EcoO109I    Hin4I     MmeI
    PpuMI     PsrI     PstI     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI