(Linear) (Six-base) MAPSORT of: KIEE6565.seq  Check: 3161  from: 1  to: 519



KIEE6565.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     519 
   Size:        455      64
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     249     488     519 
   Size:        249     239      31

  Fragments arranged by size: 

                 249     239      31
 
AseI AT'TA_AT

Cuts at:      0     449     519 
   Size:        449      70
 
BanII G_rGCy'C

Cuts at:      0     216     519 
   Size:        216     303
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      52     519 
   Size:         52     467
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     232     519 
   Size:        232     287
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     519 
   Size:        184     335
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     224     519 
   Size:        224     295
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     519 
   Size:        266     253
 
BsiHKAI G_wGCw'C

Cuts at:      0      14     216     519 
   Size:         14     202     303

  Fragments arranged by size: 

                 303     202      14
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     216     519 
   Size:         14     202     303

  Fragments arranged by size: 

                 303     202      14
 
BsrDI GCAATG_nn'

Cuts at:      0     233     519 
   Size:        233     286
 
DraI TTT'AAA

Cuts at:      0      53     519 
   Size:         53     466
 
EaeI y'GGCC_r

Cuts at:      0      95     519 
   Size:         95     424
 
EarI CTCTTCn'nnn_

Cuts at:      0     259     431     519 
   Size:        259     172      88

  Fragments arranged by size: 

                 259     172      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      43     519 
   Size:         43     476
 
EcoICRI GAG'CTC

Cuts at:      0     214     519 
   Size:        214     305
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     455     519 
   Size:        184     271      64

  Fragments arranged by size: 

                 271     184      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0     442     519 
   Size:        442      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     162     519 
   Size:        162     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309     519 
   Size:        277      32     210

  Fragments arranged by size: 

                 277     210      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     415     519 
   Size:        298     117     104

  Fragments arranged by size: 

                 298     117     104
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      54      86     519 
   Size:         54      32     433

  Fragments arranged by size: 

                 433      54      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     442     519 
   Size:        442      77
 
SacI G_AGCT'C

Cuts at:      0     216     519 
   Size:        216     303
 
SanDI GG'GwC_CC

Cuts at:      0     442     519 
   Size:        442      77
 
SapI GCTCTTCn'nnn_

Cuts at:      0     259     519 
   Size:        259     260
 
SspI AAT'ATT

Cuts at:      0     453     519 
   Size:        453      66
 

 Enzymes that do cut:

     AcuI     AlfI     AseI    BanII    BciVI     BplI     BpmI    BseRI
     BsgI  BsiHKAI Bsp1286I    BsrDI     DraI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I     MmeI     PpiI    PpuMI
     SacI    SanDI     SapI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI