Restriction map for
pFN21AE6564
(Linear) (Six-base) MAPSORT of: KIEE6564.seq Check: 3487 from: 1 to: 540
KIEE6564.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 169 540
Size: 118 51 371
Fragments arranged by size:
371 118 51
AflII C'TTAA_G
Cuts at: 0 484 540
Size: 484 56
AvaI C'yCGr_G
Cuts at: 0 197 540
Size: 197 343
BamHI G'GATC_C
Cuts at: 0 35 540
Size: 35 505
BanI G'GyrC_C
Cuts at: 0 504 540
Size: 504 36
BbvCI CC'TCA_GC
Cuts at: 0 443 540
Size: 443 97
BlpI GC'TnA_GC
Cuts at: 0 323 540
Size: 323 217
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 540
Size: 250 290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 495 540
Size: 223 272 45
Fragments arranged by size:
272 223 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 349 370 472 540
Size: 328 21 21 102 68
Fragments arranged by size:
328 102 68 21 21
Bpu10I CC'TnA_GC
Cuts at: 0 443 540
Size: 443 97
BsaHI Gr'CG_yC
Cuts at: 0 344 540
Size: 344 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 540
Size: 94 30 416
Fragments arranged by size:
416 94 30
BseYI C'CCAG_C
Cuts at: 0 426 540
Size: 426 114
BsiHKAI G_wGCw'C
Cuts at: 0 254 540
Size: 254 286
Bsp1286I G_dGCh'C
Cuts at: 0 254 540
Size: 254 286
BstXI CCAn_nnnn'nTGG
Cuts at: 0 235 540
Size: 235 305
BstYI r'GATC_y
Cuts at: 0 35 540
Size: 35 505
BtgI C'CryG_G
Cuts at: 0 159 540
Size: 159 381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 165 540
Size: 165 375
DraIII CAC_nnn'GTG
Cuts at: 0 290 540
Size: 290 250
EaeI y'GGCC_r
Cuts at: 0 2 389 540
Size: 2 387 151
Fragments arranged by size:
387 151 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 169 328 349 370 472 540
Size: 118 51 159 21 21 102 68
Fragments arranged by size:
159 118 102 68 51 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 540
Size: 226 314
EcoO109I rG'GnC_Cy
Cuts at: 0 102 280 447 540
Size: 102 178 167 93
Fragments arranged by size:
178 167 102 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 540
Size: 94 32 414
Fragments arranged by size:
414 94 32
MscI TGG'CCA
Cuts at: 0 391 540
Size: 391 149
MspA1I CmG'CkG
Cuts at: 0 21 33 540
Size: 21 12 507
Fragments arranged by size:
507 21 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 290 540
Size: 290 250
PfoI T'CCnGG_A
Cuts at: 0 449 540
Size: 449 91
PpuMI rG'GwC_Cy
Cuts at: 0 102 447 540
Size: 102 345 93
Fragments arranged by size:
345 102 93
PstI C_TGCA'G
Cuts at: 0 20 440 533 540
Size: 20 420 93 7
Fragments arranged by size:
420 93 20 7
PvuII CAG'CTG
Cuts at: 0 21 540
Size: 21 519
SbfI CC_TGCA'GG
Cuts at: 0 440 540
Size: 440 100
SfcI C'TryA_G
Cuts at: 0 16 436 529 540
Size: 16 420 93 11
Fragments arranged by size:
420 93 16 11
SmlI C'TyrA_G
Cuts at: 0 484 540
Size: 484 56
StuI AGG'CCT
Cuts at: 0 188 540
Size: 188 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 350 382 540
Size: 350 32 158
Fragments arranged by size:
350 158 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 132 540
Size: 66 66 408
Fragments arranged by size:
408 66 66
Enzymes that do cut:
AcuI AflII AvaI BamHI BanI BbvCI BlpI BmrI
BplI BpmI Bpu10I BsaHI BsaXI BseYI BsiHKAI Bsp1286I
BstXI BstYI BtgI BtgZI DraIII EaeI Eco57MI EcoNI
EcoO109I Hin4I MscI MspA1I PflMI PfoI PpuMI PstI
PvuII SbfI SfcI SmlI StuI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI XmnI ZraI