Restriction map for
pFN21AE6563
(Linear) (Six-base) MAPSORT of: KIEE6563.seq Check: 336 from: 1 to: 525
KIEE6563.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 200 525
Size: 200 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 525
Size: 286 239
ApaI G_GGCC'C
Cuts at: 0 206 525
Size: 206 319
AvaI C'yCGr_G
Cuts at: 0 296 525
Size: 296 229
BanI G'GyrC_C
Cuts at: 0 194 212 525
Size: 194 18 313
Fragments arranged by size:
313 194 18
BanII G_rGCy'C
Cuts at: 0 140 206 525
Size: 140 66 319
Fragments arranged by size:
319 140 66
BbeI G_GCGC'C
Cuts at: 0 198 525
Size: 198 327
BbvCI CC'TCA_GC
Cuts at: 0 286 525
Size: 286 239
BclI T'GATC_A
Cuts at: 0 125 525
Size: 125 400
BglI GCCn_nnn'nGGC
Cuts at: 0 358 525
Size: 358 167
Bme1580I G_kGCm'C
Cuts at: 0 20 206 525
Size: 20 186 319
Fragments arranged by size:
319 186 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 168 525
Size: 126 42 357
Fragments arranged by size:
357 126 42
Bpu10I CC'TnA_GC
Cuts at: 0 286 525
Size: 286 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 525
Size: 403 122
BsaHI Gr'CG_yC
Cuts at: 0 195 525
Size: 195 330
BsmBI CGTCTCn'nnnn_
Cuts at: 0 97 525
Size: 97 428
Bsp1286I G_dGCh'C
Cuts at: 0 20 140 206 525
Size: 20 120 66 319
Fragments arranged by size:
319 120 66 20
BspHI T'CATG_A
Cuts at: 0 330 525
Size: 330 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 200 261 525
Size: 200 61 264
Fragments arranged by size:
264 200 61
BsrBI CCG'CTC
Cuts at: 0 377 525
Size: 377 148
BstEII G'GTnAC_C
Cuts at: 0 364 525
Size: 364 161
BtgI C'CryG_G
Cuts at: 0 83 198 228 525
Size: 83 115 30 297
Fragments arranged by size:
297 115 83 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 333 525
Size: 333 192
BtsI GCAGTG_nn'
Cuts at: 0 71 398 525
Size: 71 327 127
Fragments arranged by size:
327 127 71
EaeI y'GGCC_r
Cuts at: 0 68 525
Size: 68 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 69 244 525
Size: 69 175 281
Fragments arranged by size:
281 175 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 525
Size: 286 239
EcoO109I rG'GnC_Cy
Cuts at: 0 203 282 525
Size: 203 79 243
Fragments arranged by size:
243 203 79
HaeII r_GCGC'y
Cuts at: 0 198 390 525
Size: 198 192 135
Fragments arranged by size:
198 192 135
KasI G'GCGC_C
Cuts at: 0 194 525
Size: 194 331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 525
Size: 245 280
MscI TGG'CCA
Cuts at: 0 70 525
Size: 70 455
MspA1I CmG'CkG
Cuts at: 0 123 230 525
Size: 123 107 295
Fragments arranged by size:
295 123 107
NarI GG'CG_CC
Cuts at: 0 195 525
Size: 195 330
NcoI C'CATG_G
Cuts at: 0 83 198 525
Size: 83 115 327
Fragments arranged by size:
327 115 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 50 66 525
Size: 50 16 459
Fragments arranged by size:
459 50 16
PspOMI G'GGCC_C
Cuts at: 0 202 525
Size: 202 323
PvuII CAG'CTG
Cuts at: 0 123 525
Size: 123 402
SacII CC_GC'GG
Cuts at: 0 231 525
Size: 231 294
SfoI GGC'GCC
Cuts at: 0 196 525
Size: 196 329
SmaI CCC'GGG
Cuts at: 0 298 525
Size: 298 227
StyI C'CwwG_G
Cuts at: 0 83 198 525
Size: 83 115 327
Fragments arranged by size:
327 115 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 525
Size: 145 380
XmaI C'CCGG_G
Cuts at: 0 296 525
Size: 296 229
Enzymes that do cut:
AarI AcuI ApaI AvaI BanI BanII BbeI BbvCI
BclI BglI Bme1580I BplI Bpu10I BsaI BsaHI BsmBI
Bsp1286I BspHI BspMI BsrBI BstEII BtgI BtgZI BtsI
EaeI EciI Eco57MI EcoO109I HaeII KasI MmeI MscI
MspA1I NarI NcoI PflMI PspOMI PvuII SacII SfoI
SmaI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI BpmI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI