(Linear) (Six-base) MAPSORT of: KIEE6559.seq  Check: 6161  from: 1  to: 480



KIEE6559.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     480 
   Size:        136     344
 
AlwNI CAG_nnn'CTG

Cuts at:      0     449     480 
   Size:        449      31
 
ApoI r'AATT_y

Cuts at:      0     283     480 
   Size:        283     197
 
BanI G'GyrC_C

Cuts at:      0      56      97     310     480 
   Size:         56      41     213     170

  Fragments arranged by size: 

                 213     170      56      41
 
BbeI G_GCGC'C

Cuts at:      0     101     480 
   Size:        101     379
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     403     480 
   Size:        403      77
 
BbvCI CC'TCA_GC

Cuts at:      0      10     480 
   Size:         10     470
 
BglII A'GATC_T

Cuts at:      0     119     480 
   Size:        119     361
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     388     480 
   Size:        388      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     443     480 
   Size:        124     319      37

  Fragments arranged by size: 

                 319     124      37
 
Bpu10I CC'TnA_GC

Cuts at:      0      10     480 
   Size:         10     470
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      78     480 
   Size:         78     402
 
BsaHI Gr'CG_yC

Cuts at:      0      98     480 
   Size:         98     382
 
BsaWI w'CCGG_w

Cuts at:      0     155     480 
   Size:        155     325
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     119     121     480 
   Size:        119       2     359

  Fragments arranged by size: 

                 359     119       2
 
BspEI T'CCGG_A

Cuts at:      0     155     480 
   Size:        155     325
 
BsrBI CCG'CTC

Cuts at:      0     316     480 
   Size:        316     164
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     449     480 
   Size:        449      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     480 
   Size:        396      84
 
BstYI r'GATC_y

Cuts at:      0     119     200     480 
   Size:        119      81     280

  Fragments arranged by size: 

                 280     119      81
 
EaeI y'GGCC_r

Cuts at:      0      45     335     386     480 
   Size:         45     290      51      94

  Fragments arranged by size: 

                 290      94      51      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     136     443     480 
   Size:        124      12     307      37

  Fragments arranged by size: 

                 307     124      37      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     236     480 
   Size:        236     244
 
HaeII r_GCGC'y

Cuts at:      0     101     480 
   Size:        101     379
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     111     143     387     419     420     452     480 
   Size:        111      32     244      32       1      32      28

  Fragments arranged by size: 

                 244     111      32      32      32      28       1
 
KasI G'GCGC_C

Cuts at:      0      97     480 
   Size:         97     383
 
MscI TGG'CCA

Cuts at:      0     337     388     480 
   Size:        337      51      92

  Fragments arranged by size: 

                 337      92      51
 
NarI GG'CG_CC

Cuts at:      0      98     480 
   Size:         98     382
 
PstI C_TGCA'G

Cuts at:      0      86     445     480 
   Size:         86     359      35

  Fragments arranged by size: 

                 359      86      35
 
SfcI C'TryA_G

Cuts at:      0      82     441     480 
   Size:         82     359      39

  Fragments arranged by size: 

                 359      82      39
 
SfoI GGC'GCC

Cuts at:      0      99     480 
   Size:         99     381
 
TatI w'GTAC_w

Cuts at:      0     134     480 
   Size:        134     346
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI     BbeI     BbsI    BbvCI    BglII
     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BseRI    BspEI
    BsrBI   BstAPI    BstXI    BstYI     EaeI  Eco57MI EcoO109I    HaeII
    Hin4I     KasI     MscI     NarI     PstI     SfcI     SfoI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI    BpuEI    BsaAI    BsaBI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI