(Linear) (Six-base) MAPSORT of: KIEE6557.seq  Check: 1007  from: 1  to: 459



KIEE6557.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0     306     459 
   Size:        306     153
 
BbvCI CC'TCA_GC

Cuts at:      0     266     459 
   Size:        266     193
 
BglI GCCn_nnn'nGGC

Cuts at:      0     349     459 
   Size:        349     110
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     459 
   Size:         16     443
 
Bpu10I CC'TnA_GC

Cuts at:      0     266     459 
   Size:        266     193
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     258     459 
   Size:        258     201
 
BsmI GAATG_Cn'

Cuts at:      0      92     459 
   Size:         92     367
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     151     459 
   Size:        151     308
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     400     459 
   Size:        400      59
 
BstYI r'GATC_y

Cuts at:      0      43     459 
   Size:         43     416
 
EarI CTCTTCn'nnn_

Cuts at:      0     147     178     459 
   Size:        147      31     281

  Fragments arranged by size: 

                 281     147      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     459 
   Size:         16     443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     459 
   Size:        285     174
 
StuI AGG'CCT

Cuts at:      0     352     459 
   Size:        352     107
 
StyI C'CwwG_G

Cuts at:      0     247     459 
   Size:        247     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       7     459 
   Size:          7     452
 
XbaI T'CTAG_A

Cuts at:      0     375     459 
   Size:        375      84
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      54     459 
   Size:         54     405
 

 Enzymes that do cut:

     BbsI    BbvCI     BglI     BpmI   Bpu10I     BsaI     BsmI    BsmBI
    BspMI    BstYI     EarI  Eco57MI     MmeI     StuI     StyI    TaqII
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XhoI     XmaI
     XmnI     ZraI