Restriction map for
pFN21AE6555
(Linear) (Six-base) MAPSORT of: KIEE6555.seq Check: 9855 from: 1 to: 462
KIEE6555.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AgeI A'CCGG_T
Cuts at: 0 154 462
Size: 154 308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 58 462
Size: 58 404
AlwNI CAG_nnn'CTG
Cuts at: 0 243 462
Size: 243 219
ApaLI G'TGCA_C
Cuts at: 0 127 462
Size: 127 335
BanII G_rGCy'C
Cuts at: 0 101 462
Size: 101 361
BbvCI CC'TCA_GC
Cuts at: 0 304 462
Size: 304 158
BglI GCCn_nnn'nGGC
Cuts at: 0 10 462
Size: 10 452
BlpI GC'TnA_GC
Cuts at: 0 79 462
Size: 79 383
Bme1580I G_kGCm'C
Cuts at: 0 131 405 462
Size: 131 274 57
Fragments arranged by size:
274 131 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 160 462
Size: 160 302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 462
Size: 202 260
Bpu10I CC'TnA_GC
Cuts at: 0 304 462
Size: 304 158
BsaBI GATnn'nnATC
Cuts at: 0 456 462
Size: 456 6
BsaHI Gr'CG_yC
Cuts at: 0 68 462
Size: 68 394
BsaWI w'CCGG_w
Cuts at: 0 154 462
Size: 154 308
BseYI C'CCAG_C
Cuts at: 0 83 462
Size: 83 379
BsiHKAI G_wGCw'C
Cuts at: 0 131 462
Size: 131 331
Bsp1286I G_dGCh'C
Cuts at: 0 101 131 405 462
Size: 101 30 274 57
Fragments arranged by size:
274 101 57 30
BspHI T'CATG_A
Cuts at: 0 255 462
Size: 255 207
BsrBI CCG'CTC
Cuts at: 0 78 462
Size: 78 384
BsrFI r'CCGG_y
Cuts at: 0 64 154 462
Size: 64 90 308
Fragments arranged by size:
308 90 64
BstYI r'GATC_y
Cuts at: 0 87 119 462
Size: 87 32 343
Fragments arranged by size:
343 87 32
Bsu36I CC'TnA_GG
Cuts at: 0 229 462
Size: 229 233
EarI CTCTTCn'nnn_
Cuts at: 0 52 169 462
Size: 52 117 293
Fragments arranged by size:
293 117 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 462
Size: 202 260
EcoNI CCTnn'n_nnAGG
Cuts at: 0 228 462
Size: 228 234
EcoO109I rG'GnC_Cy
Cuts at: 0 428 462
Size: 428 34
HaeII r_GCGC'y
Cuts at: 0 23 462
Size: 23 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 462
Size: 243 32 187
Fragments arranged by size:
243 187 32
MslI CAynn'nnrTG
Cuts at: 0 450 462
Size: 450 12
MspA1I CmG'CkG
Cuts at: 0 83 249 462
Size: 83 166 213
Fragments arranged by size:
213 166 83
NaeI GCC'GGC
Cuts at: 0 66 462
Size: 66 396
NgoMIV G'CCGG_C
Cuts at: 0 64 462
Size: 64 398
NruI TCG'CGA
Cuts at: 0 135 462
Size: 135 327
PasI CC'CwG_GG
Cuts at: 0 409 462
Size: 409 53
PfoI T'CCnGG_A
Cuts at: 0 92 326 462
Size: 92 234 136
Fragments arranged by size:
234 136 92
PpuMI rG'GwC_Cy
Cuts at: 0 428 462
Size: 428 34
PstI C_TGCA'G
Cuts at: 0 248 354 462
Size: 248 106 108
Fragments arranged by size:
248 108 106
PvuII CAG'CTG
Cuts at: 0 83 249 462
Size: 83 166 213
Fragments arranged by size:
213 166 83
SanDI GG'GwC_CC
Cuts at: 0 428 462
Size: 428 34
SapI GCTCTTCn'nnn_
Cuts at: 0 52 462
Size: 52 410
SfcI C'TryA_G
Cuts at: 0 244 350 462
Size: 244 106 112
Fragments arranged by size:
244 112 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 422 462
Size: 422 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 462
Size: 308 32 122
Fragments arranged by size:
308 122 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 334 462
Size: 334 128
Enzymes that do cut:
AgeI AlfI AlwNI ApaLI BanII BbvCI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaBI BsaHI BsaWI BseYI
BsiHKAI Bsp1286I BspHI BsrBI BsrFI BstYI Bsu36I EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I MslI MspA1I NaeI
NgoMIV NruI PasI PfoI PpuMI PstI PvuII SanDI
SapI SfcI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI