(Linear) (Six-base) MAPSORT of: KIEE6552.seq  Check: 7589  from: 1  to: 384



KIEE6552.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AccI GT'mk_AC

Cuts at:      0     191     384 
   Size:        191     193
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     384 
   Size:         88     296
 
AlwNI CAG_nnn'CTG

Cuts at:      0      42      81     384 
   Size:         42      39     303

  Fragments arranged by size: 

                 303      42      39
 
ApoI r'AATT_y

Cuts at:      0     133     145     364     384 
   Size:        133      12     219      20

  Fragments arranged by size: 

                 219     133      20      12
 
AvaI C'yCGr_G

Cuts at:      0      89     384 
   Size:         89     295
 
BanII G_rGCy'C

Cuts at:      0      88     384 
   Size:         88     296
 
BclI T'GATC_A

Cuts at:      0     372     384 
   Size:        372      12
 
BlpI GC'TnA_GC

Cuts at:      0      82     384 
   Size:         82     302
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     359     384 
   Size:        352       7      25

  Fragments arranged by size: 

                 352      25       7
 
BsaBI GATnn'nnATC

Cuts at:      0     377     384 
   Size:        377       7
 
BsaWI w'CCGG_w

Cuts at:      0      15     384 
   Size:         15     369
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     384 
   Size:         88     296
 
BspEI T'CCGG_A

Cuts at:      0      15     384 
   Size:         15     369
 
BsrFI r'CCGG_y

Cuts at:      0      96     384 
   Size:         96     288
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      42     384 
   Size:         42     342
 
BstZ17I GTA'TAC

Cuts at:      0     192     384 
   Size:        192     192
 
BtgI C'CryG_G

Cuts at:      0     183     384 
   Size:        183     201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      75     384 
   Size:         75     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     352     359     384 
   Size:         88     264       7      25

  Fragments arranged by size: 

                 264      88      25       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     121     384 
   Size:        121     263
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     222     384 
   Size:        222     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177     384 
   Size:        145      32     207

  Fragments arranged by size: 

                 207     145      32
 
MspA1I CmG'CkG

Cuts at:      0      42     384 
   Size:         42     342
 
NcoI C'CATG_G

Cuts at:      0     183     384 
   Size:        183     201
 
NdeI CA'TA_TG

Cuts at:      0     282     384 
   Size:        282     102
 
PstI C_TGCA'G

Cuts at:      0      38     384 
   Size:         38     346
 
PvuII CAG'CTG

Cuts at:      0      42     384 
   Size:         42     342
 
SfcI C'TryA_G

Cuts at:      0      34     384 
   Size:         34     350
 
SspI AAT'ATT

Cuts at:      0     311     384 
   Size:        311      73
 
StyI C'CwwG_G

Cuts at:      0     166     183     384 
   Size:        166      17     201

  Fragments arranged by size: 

                 201     166      17
 
TatI w'GTAC_w

Cuts at:      0     222     384 
   Size:        222     162
 
XmnI GAAnn'nnTTC

Cuts at:      0     347     384 
   Size:        347      37
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AvaI    BanII     BclI     BlpI
     BpmI    BsaBI    BsaWI Bsp1286I    BspEI    BsrFI   BstAPI  BstZ17I
     BtgI    BtgZI  Eco57MI EcoO109I     FalI    Hin4I   MspA1I     NcoI
     NdeI     PstI    PvuII     SfcI     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI