(Linear) (Six-base) MAPSORT of: KIEE6549.seq  Check: 9887  from: 1  to: 348



KIEE6549.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     348 
   Size:        117     231
 
AseI AT'TA_AT

Cuts at:      0     193     348 
   Size:        193     155
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     348 
   Size:         77     271
 
BsaBI GATnn'nnATC

Cuts at:      0     242     348 
   Size:        242     106
 
BsrDI GCAATG_nn'

Cuts at:      0      32     348 
   Size:         32     316
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      49     348 
   Size:         49     299
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      12     348 
   Size:         12     336
 
DraI TTT'AAA

Cuts at:      0     263     348 
   Size:        263      85
 
EaeI y'GGCC_r

Cuts at:      0       2     348 
   Size:          2     346
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     348 
   Size:        117     231
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     275     348 
   Size:        275      73
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     348 
   Size:          3     345
 
MscI TGG'CCA

Cuts at:      0       4     348 
   Size:          4     344
 
NdeI CA'TA_TG

Cuts at:      0      83     348 
   Size:         83     265
 
PstI C_TGCA'G

Cuts at:      0      45     348 
   Size:         45     303
 
SfcI C'TryA_G

Cuts at:      0      41     348 
   Size:         41     307
 
SmlI C'TyrA_G

Cuts at:      0      92     348 
   Size:         92     256
 
TatI w'GTAC_w

Cuts at:      0       7     348 
   Size:          7     341
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      81     348 
   Size:         81     267
 

 Enzymes that do cut:

     AcuI     AseI    BpuEI    BsaBI    BsrDI   BstAPI    BstXI     DraI
     EaeI  Eco57MI    EcoNI     MmeI     MscI     NdeI     PstI     SfcI
     SmlI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI