(Linear) (Six-base) MAPSORT of: KIEE6548.seq  Check: 9716  from: 1  to: 324



KIEE6548.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     324 
   Size:         64     260
 
AlwNI CAG_nnn'CTG

Cuts at:      0     184     324 
   Size:        184     140
 
ApoI r'AATT_y

Cuts at:      0     141     277     324 
   Size:        141     136      47

  Fragments arranged by size: 

                 141     136      47
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      52     324 
   Size:         52     272
 
BbvCI CC'TCA_GC

Cuts at:      0     290     324 
   Size:        290      34
 
BlpI GC'TnA_GC

Cuts at:      0     188     324 
   Size:        188     136
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172     324 
   Size:        172     152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     324 
   Size:         40     284
 
Bpu10I CC'TnA_GC

Cuts at:      0     290     324 
   Size:        290      34
 
BsaAI yAC'GTr

Cuts at:      0     242     324 
   Size:        242      82
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     288     324 
   Size:        288      36
 
BspHI T'CATG_A

Cuts at:      0     228     324 
   Size:        228      96
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     227     324 
   Size:        227      97
 
Bsu36I CC'TnA_GG

Cuts at:      0     300     324 
   Size:        300      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     112     324 
   Size:        112     212
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      64     324 
   Size:         40      24     260

  Fragments arranged by size: 

                 260      40      24
 
EcoRI G'AATT_C

Cuts at:      0     141     324 
   Size:        141     183
 
HaeII r_GCGC'y

Cuts at:      0      43     324 
   Size:         43     281
 
PfoI T'CCnGG_A

Cuts at:      0      56     324 
   Size:         56     268
 
PstI C_TGCA'G

Cuts at:      0     224     324 
   Size:        224     100
 
SfcI C'TryA_G

Cuts at:      0     220     234     324 
   Size:        220      14      90

  Fragments arranged by size: 

                 220      90      14
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BbsI    BbvCI     BlpI     BmrI     BpmI
   Bpu10I    BsaAI    BseRI    BspHI    BspMI   Bsu36I     EarI  Eco57MI
    EcoRI    HaeII     PfoI     PstI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI