(Linear) (Six-base) MAPSORT of: KIEE6546.seq  Check: 5290  from: 1  to: 318



KIEE6546.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

Acc65I G'GTAC_C

Cuts at:      0     229     318 
   Size:        229      89
 
ApaLI G'TGCA_C

Cuts at:      0     128     318 
   Size:        128     190
 
AvaI C'yCGr_G

Cuts at:      0     149     318 
   Size:        149     169
 
BanI G'GyrC_C

Cuts at:      0     229     318 
   Size:        229      89
 
Bme1580I G_kGCm'C

Cuts at:      0     132     318 
   Size:        132     186
 
BmgBI CAC'GTC

Cuts at:      0     223     318 
   Size:        223      95
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     318 
   Size:        245      73
 
BsiHKAI G_wGCw'C

Cuts at:      0     132     318 
   Size:        132     186
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     213     318 
   Size:        213     105
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     318 
   Size:        132     186
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     217     318 
   Size:        217     101
 
ClaI AT'CG_AT

Cuts at:      0      45     318 
   Size:         45     273
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     318 
   Size:         75      32     211

  Fragments arranged by size: 

                 211      75      32
 
HincII GTy'rAC

Cuts at:      0      53     318 
   Size:         53     265
 
KpnI G_GTAC'C

Cuts at:      0     233     318 
   Size:        233      85
 
MspA1I CmG'CkG

Cuts at:      0     246     318 
   Size:        246      72
 
TatI w'GTAC_w

Cuts at:      0     272     318 
   Size:        272      46
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     111     143     318 
   Size:        111      32     175

  Fragments arranged by size: 

                 175     111      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      25     318 
   Size:         25     293
 

 Enzymes that do cut:

   Acc65I    ApaLI     AvaI     BanI Bme1580I    BmgBI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspMI     ClaI    Hin4I   HincII     KpnI   MspA1I
     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI