(Linear) (Six-base) MAPSORT of: KIEE6545.seq  Check: 1143  from: 1  to: 303



KIEE6545.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

ApaLI G'TGCA_C

Cuts at:      0     193     303 
   Size:        193     110
 
ApoI r'AATT_y

Cuts at:      0     176     303 
   Size:        176     127
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     267     303 
   Size:        267      36
 
Bme1580I G_kGCm'C

Cuts at:      0     197     303 
   Size:        197     106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     303 
   Size:         29     274
 
BsiHKAI G_wGCw'C

Cuts at:      0     197     303 
   Size:        197     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     197     303 
   Size:        197     106
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      53     303 
   Size:         53     250
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     265     303 
   Size:        265      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     303 
   Size:         29     274
 
HindIII A'AGCT_T

Cuts at:      0     113     303 
   Size:        113     190
 
NdeI CA'TA_TG

Cuts at:      0     147     303 
   Size:        147     156
 
SexAI A'CCwGG_T

Cuts at:      0     201     303 
   Size:        201     102
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      48      80     303 
   Size:         48      32     223

  Fragments arranged by size: 

                 223      48      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     160     303 
   Size:        160     143
 

 Enzymes that do cut:

    ApaLI     ApoI    BciVI Bme1580I     BpmI  BsiHKAI Bsp1286I    BstXI
    BtgZI  Eco57MI  HindIII     NdeI    SexAI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI