(Linear) (Six-base) MAPSORT of: KIEE6544.seq  Check: 4697  from: 1  to: 321



KIEE6544.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     212     244     321 
   Size:        212      32      77

  Fragments arranged by size: 

                 212      77      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     321 
   Size:        120     201
 
ApaI G_GGCC'C

Cuts at:      0     148     321 
   Size:        148     173
 
BamHI G'GATC_C

Cuts at:      0       4     321 
   Size:          4     317
 
BanII G_rGCy'C

Cuts at:      0     148     321 
   Size:        148     173
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     260     321 
   Size:        260      61
 
Bme1580I G_kGCm'C

Cuts at:      0     148     321 
   Size:        148     173
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     240     321 
   Size:        240      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     321 
   Size:        239      82
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     169     199     212     242     321 
   Size:        169      30      13      30      79

  Fragments arranged by size: 

                 169      79      30      30      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     111     321 
   Size:         34      77     210

  Fragments arranged by size: 

                 210      77      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     321 
   Size:        148     173
 
BspHI T'CATG_A

Cuts at:      0     288     321 
   Size:        288      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     218     321 
   Size:        218     103
 
BstYI r'GATC_y

Cuts at:      0       4     321 
   Size:          4     317
 
EaeI y'GGCC_r

Cuts at:      0     295     321 
   Size:        295      26
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     321 
   Size:         65     256
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     321 
   Size:        239      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0     136     321 
   Size:        136     185
 
MspA1I CmG'CkG

Cuts at:      0     172     279     321 
   Size:        172     107      42

  Fragments arranged by size: 

                 172     107      42
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     212     244     321 
   Size:        212      32      77

  Fragments arranged by size: 

                 212      77      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     136     321 
   Size:        136     185
 
PspOMI G'GGCC_C

Cuts at:      0     144     321 
   Size:        144     177
 
PvuII CAG'CTG

Cuts at:      0     172     279     321 
   Size:        172     107      42

  Fragments arranged by size: 

                 172     107      42
 
SfcI C'TryA_G

Cuts at:      0     167     321 
   Size:        167     154
 
StyI C'CwwG_G

Cuts at:      0      51     131     321 
   Size:         51      80     190

  Fragments arranged by size: 

                 190      80      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28     321 
   Size:         28     293
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138     321 
   Size:        138     183
 

 Enzymes that do cut:

     AloI    AlwNI     ApaI    BamHI    BanII    BciVI Bme1580I     BmrI
     BpmI    BsaXI     BsgI Bsp1286I    BspHI    BspMI    BstYI     EaeI
     EarI  Eco57MI EcoO109I   MspA1I     PpiI    PpuMI   PspOMI    PvuII
     SfcI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI