(Linear) (Six-base) MAPSORT of: KIEE6540.seq  Check: 4817  from: 1  to: 255



KIEE6540.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      64     255 
   Size:         64     191
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     183     216     255 
   Size:        183      33      39

  Fragments arranged by size: 

                 183      39      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     122     255 
   Size:        122     133
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20     255 
   Size:         20     235
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      22     255 
   Size:         22     233
 
BsrGI T'GTAC_A

Cuts at:      0      31     255 
   Size:         31     224
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      68     103     255 
   Size:         68      35     152

  Fragments arranged by size: 

                 152      68      35
 
EaeI y'GGCC_r

Cuts at:      0     122     255 
   Size:        122     133
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     255 
   Size:        219      36
 
MscI TGG'CCA

Cuts at:      0     124     255 
   Size:        124     131
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157     255 
   Size:        157      98
 
PstI C_TGCA'G

Cuts at:      0      97     255 
   Size:         97     158
 
SfcI C'TryA_G

Cuts at:      0      48      93     255 
   Size:         48      45     162

  Fragments arranged by size: 

                 162      48      45
 
TatI w'GTAC_w

Cuts at:      0      31     255 
   Size:         31     224
 

 Enzymes that do cut:

    AlwNI     BaeI    BciVI     BmrI     BsaI    BsrGI    CspCI     EaeI
     EarI     MscI    PflMI     PstI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI