(Linear) (Six-base) MAPSORT of: KIEE6538.seq  Check: 2992  from: 1  to: 264



KIEE6538.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     264 
   Size:        140     124
 
AhdI GACnn_n'nnGTC

Cuts at:      0     199     264 
   Size:        199      65
 
AlwNI CAG_nnn'CTG

Cuts at:      0     196     264 
   Size:        196      68
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43     264 
   Size:         43     221
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     169     264 
   Size:        142      27      95

  Fragments arranged by size: 

                 142      95      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      76     204     264 
   Size:         76     128      60

  Fragments arranged by size: 

                 128      76      60
 
EaeI y'GGCC_r

Cuts at:      0      76     264 
   Size:         76     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     264 
   Size:        140     124
 
EcoO109I rG'GnC_Cy

Cuts at:      0     193     264 
   Size:        193      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     185     217     264 
   Size:        185      32      47

  Fragments arranged by size: 

                 185      47      32
 
HincII GTy'rAC

Cuts at:      0      91     264 
   Size:         91     173
 
PfoI T'CCnGG_A

Cuts at:      0     171     189     264 
   Size:        171      18      75

  Fragments arranged by size: 

                 171      75      18
 
PpuMI rG'GwC_Cy

Cuts at:      0     193     264 
   Size:        193      71
 
PstI C_TGCA'G

Cuts at:      0     201     264 
   Size:        201      63
 
SfcI C'TryA_G

Cuts at:      0     197     264 
   Size:        197      67
 
SspI AAT'ATT

Cuts at:      0      57     225     264 
   Size:         57     168      39

  Fragments arranged by size: 

                 168      57      39
 
TatI w'GTAC_w

Cuts at:      0     112     264 
   Size:        112     152
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     BglI    BseRI    BspMI     EaeI  Eco57MI
 EcoO109I    Hin4I   HincII     PfoI    PpuMI     PstI     SfcI     SspI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI