(Linear) (Six-base) MAPSORT of: KIEE6534.seq  Check: 7110  from: 1  to: 192



KIEE6534.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     192 
   Size:         70     122
 
ApoI r'AATT_y

Cuts at:      0      55     192 
   Size:         55     137
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      16      50     192 
   Size:         16      34     142

  Fragments arranged by size: 

                 142      34      16
 
BlpI GC'TnA_GC

Cuts at:      0      84     192 
   Size:         84     108
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      23      53     192 
   Size:         23      30     139

  Fragments arranged by size: 

                 139      30      23
 
BseYI C'CCAG_C

Cuts at:      0     170     192 
   Size:        170      22
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     192 
   Size:        164      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     192 
   Size:        164      28
 
BspHI T'CATG_A

Cuts at:      0     164     192 
   Size:        164      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      88     192 
   Size:         88     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     192 
   Size:         70     122
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     168     192 
   Size:        168      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      23      55     106     119     138     151     192 
   Size:         23      32      51      13      19      13      41

  Fragments arranged by size: 

                  51      41      32      23      19      13      13
 
XmnI GAAnn'nnTTC

Cuts at:      0     151     192 
   Size:        151      41
 

 Enzymes that do cut:

     AcuI     ApoI     BcgI     BlpI    BsaXI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI  Eco57MI     FalI    Hin4I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI