Restriction map for
pFN21AE6532
(Linear) (Six-base) MAPSORT of: KIEE6532.seq Check: 505 from: 1 to: 552
KIEE6532.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 552
Size: 160 392
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 552
Size: 259 32 261
Fragments arranged by size:
261 259 32
ApaI G_GGCC'C
Cuts at: 0 81 552
Size: 81 471
BamHI G'GATC_C
Cuts at: 0 274 552
Size: 274 278
BanII G_rGCy'C
Cuts at: 0 81 329 552
Size: 81 248 223
Fragments arranged by size:
248 223 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 552
Size: 302 250
BglI GCCn_nnn'nGGC
Cuts at: 0 166 552
Size: 166 386
Bme1580I G_kGCm'C
Cuts at: 0 81 552
Size: 81 471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 514 552
Size: 514 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 377 509 552
Size: 343 34 132 43
Fragments arranged by size:
343 132 43 34
Bpu10I CC'TnA_GC
Cuts at: 0 164 552
Size: 164 388
BsaI GGTCTCn'nnnn_
Cuts at: 0 353 552
Size: 353 199
BsaAI yAC'GTr
Cuts at: 0 22 552
Size: 22 530
BsaBI GATnn'nnATC
Cuts at: 0 337 552
Size: 337 215
BsaWI w'CCGG_w
Cuts at: 0 362 552
Size: 362 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 448 552
Size: 280 168 104
Fragments arranged by size:
280 168 104
BsiHKAI G_wGCw'C
Cuts at: 0 227 552
Size: 227 325
Bsp1286I G_dGCh'C
Cuts at: 0 81 227 329 552
Size: 81 146 102 223
Fragments arranged by size:
223 146 102 81
BspHI T'CATG_A
Cuts at: 0 203 471 552
Size: 203 268 81
Fragments arranged by size:
268 203 81
BsrBI CCG'CTC
Cuts at: 0 10 163 552
Size: 10 153 389
Fragments arranged by size:
389 153 10
BsrDI GCAATG_nn'
Cuts at: 0 176 413 552
Size: 176 237 139
Fragments arranged by size:
237 176 139
BsrFI r'CCGG_y
Cuts at: 0 105 552
Size: 105 447
BsrGI T'GTAC_A
Cuts at: 0 173 552
Size: 173 379
BstYI r'GATC_y
Cuts at: 0 274 552
Size: 274 278
BtgI C'CryG_G
Cuts at: 0 82 195 552
Size: 82 113 357
Fragments arranged by size:
357 113 82
BtsI GCAGTG_nn'
Cuts at: 0 268 552
Size: 268 284
DraIII CAC_nnn'GTG
Cuts at: 0 255 552
Size: 255 297
EaeI y'GGCC_r
Cuts at: 0 2 552
Size: 2 550
EarI CTCTTCn'nnn_
Cuts at: 0 316 552
Size: 316 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 47 124 552
Size: 47 77 428
Fragments arranged by size:
428 77 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 377 509 552
Size: 343 34 132 43
Fragments arranged by size:
343 132 43 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 509 552
Size: 509 43
EcoO109I rG'GnC_Cy
Cuts at: 0 78 552
Size: 78 474
MscI TGG'CCA
Cuts at: 0 4 552
Size: 4 548
MslI CAynn'nnrTG
Cuts at: 0 418 552
Size: 418 134
MspA1I CmG'CkG
Cuts at: 0 84 129 197 408 552
Size: 84 45 68 211 144
Fragments arranged by size:
211 144 84 68 45
NaeI GCC'GGC
Cuts at: 0 107 552
Size: 107 445
NgoMIV G'CCGG_C
Cuts at: 0 105 552
Size: 105 447
PasI CC'CwG_GG
Cuts at: 0 74 552
Size: 74 478
PspOMI G'GGCC_C
Cuts at: 0 77 552
Size: 77 475
PstI C_TGCA'G
Cuts at: 0 380 552
Size: 380 172
RsrII CG'GwC_CG
Cuts at: 0 365 552
Size: 365 187
SacII CC_GC'GG
Cuts at: 0 85 198 552
Size: 85 113 354
Fragments arranged by size:
354 113 85
SfcI C'TryA_G
Cuts at: 0 376 552
Size: 376 176
SgrAI Cr'CCGG_yG
Cuts at: 0 105 552
Size: 105 447
SnaBI TAC'GTA
Cuts at: 0 22 552
Size: 22 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 552
Size: 382 170
TatI w'GTAC_w
Cuts at: 0 173 552
Size: 173 379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 552
Size: 206 32 314
Fragments arranged by size:
314 206 32
Enzymes that do cut:
AlfI AloI ApaI BamHI BanII BbsI BglI Bme1580I
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsgI
BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstYI
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I MscI MslI MspA1I NaeI NgoMIV PasI PspOMI
PstI RsrII SacII SfcI SgrAI SnaBI TaqII TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BpuEI BsaHI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI