(Linear) (Six-base) MAPSORT of: KIEE6531.seq  Check: 8322  from: 1  to: 594



KIEE6531.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

Acc65I G'GTAC_C

Cuts at:      0     422     594 
   Size:        422     172
 
ApoI r'AATT_y

Cuts at:      0     280     415     594 
   Size:        280     135     179

  Fragments arranged by size: 

                 280     179     135
 
AvrII C'CTAG_G

Cuts at:      0      79     594 
   Size:         79     515
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     414     447     594 
   Size:        414      33     147

  Fragments arranged by size: 

                 414     147      33
 
BanI G'GyrC_C

Cuts at:      0     422     594 
   Size:        422     172
 
BanII G_rGCy'C

Cuts at:      0      34     594 
   Size:         34     560
 
BbvCI CC'TCA_GC

Cuts at:      0      59     594 
   Size:         59     535
 
Bme1580I G_kGCm'C

Cuts at:      0     231     594 
   Size:        231     363
 
Bpu10I CC'TnA_GC

Cuts at:      0      59     594 
   Size:         59     535
 
BsaAI yAC'GTr

Cuts at:      0     558     594 
   Size:        558      36
 
BsaHI Gr'CG_yC

Cuts at:      0      76     594 
   Size:         76     518
 
BsaWI w'CCGG_w

Cuts at:      0      69     594 
   Size:         69     525
 
BseYI C'CCAG_C

Cuts at:      0     524     594 
   Size:        524      70
 
Bsp1286I G_dGCh'C

Cuts at:      0      34     231     594 
   Size:         34     197     363

  Fragments arranged by size: 

                 363     197      34
 
BsrDI GCAATG_nn'

Cuts at:      0     509     594 
   Size:        509      85
 
BstYI r'GATC_y

Cuts at:      0     500     594 
   Size:        500      94
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     526     594 
   Size:        526      68
 
EaeI y'GGCC_r

Cuts at:      0     471     594 
   Size:        471     123
 
EarI CTCTTCn'nnn_

Cuts at:      0     217     594 
   Size:        217     377
 
KpnI G_GTAC'C

Cuts at:      0     426     594 
   Size:        426     168
 
MfeI C'AATT_G

Cuts at:      0     197     594 
   Size:        197     397
 
MscI TGG'CCA

Cuts at:      0     473     594 
   Size:        473     121
 
MspA1I CmG'CkG

Cuts at:      0     524     594 
   Size:        524      70
 
NspI r_CATG'y

Cuts at:      0     105     594 
   Size:        105     489
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     497     594 
   Size:        497      97
 
PsiI TTA'TAA

Cuts at:      0     434     445     594 
   Size:        434      11     149

  Fragments arranged by size: 

                 434     149      11
 
PvuII CAG'CTG

Cuts at:      0     524     594 
   Size:        524      70
 
SspI AAT'ATT

Cuts at:      0     486     594 
   Size:        486     108
 
StuI AGG'CCT

Cuts at:      0      96     594 
   Size:         96     498
 
StyI C'CwwG_G

Cuts at:      0      79     594 
   Size:         79     515
 
XmnI GAAnn'nnTTC

Cuts at:      0     280     594 
   Size:        280     314
 

 Enzymes that do cut:

   Acc65I     ApoI    AvrII     BaeI     BanI    BanII    BbvCI Bme1580I
   Bpu10I    BsaAI    BsaHI    BsaWI    BseYI Bsp1286I    BsrDI    BstYI
     DrdI     EaeI     EarI     KpnI     MfeI     MscI   MspA1I     NspI
    PflMI     PsiI    PvuII     SspI     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    BamHI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI