(Linear) (Six-base) MAPSORT of: KIEE6529.seq  Check: 2309  from: 1  to: 639



KIEE6529.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AccI GT'mk_AC

Cuts at:      0     292     639 
   Size:        292     347
 
AflII C'TTAA_G

Cuts at:      0     471     639 
   Size:        471     168
 
ApoI r'AATT_y

Cuts at:      0     388     436     639 
   Size:        388      48     203

  Fragments arranged by size: 

                 388     203      48
 
AvaI C'yCGr_G

Cuts at:      0     266     639 
   Size:        266     373
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     568     639 
   Size:        568      71
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     185     219     639 
   Size:        185      34     420

  Fragments arranged by size: 

                 420     185      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     280     639 
   Size:        280     359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     639 
   Size:        168     471
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       1     181     639 
   Size:          1     180     458

  Fragments arranged by size: 

                 458     180       1
 
BsmI GAATG_Cn'

Cuts at:      0     435     639 
   Size:        435     204
 
BspHI T'CATG_A

Cuts at:      0     423     639 
   Size:        423     216
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      92     639 
   Size:         92     547
 
BsrDI GCAATG_nn'

Cuts at:      0     566     639 
   Size:        566      73
 
BsrGI T'GTAC_A

Cuts at:      0     547     639 
   Size:        547      92
 
BssSI C'ACGA_G

Cuts at:      0      59     639 
   Size:         59     580
 
BstBI TT'CG_AA

Cuts at:      0     239     639 
   Size:        239     400
 
BtgI C'CryG_G

Cuts at:      0       4     639 
   Size:          4     635
 
BtsI GCAGTG_nn'

Cuts at:      0      87     639 
   Size:         87     552
 
DraI TTT'AAA

Cuts at:      0     594     639 
   Size:        594      45
 
EaeI y'GGCC_r

Cuts at:      0     135     639 
   Size:        135     504
 
EarI CTCTTCn'nnn_

Cuts at:      0     267     639 
   Size:        267     372
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     639 
   Size:        168     471
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     577     639 
   Size:        577      62
 
MslI CAynn'nnrTG

Cuts at:      0     428     639 
   Size:        428     211
 
MspA1I CmG'CkG

Cuts at:      0       6     639 
   Size:          6     633
 
NspI r_CATG'y

Cuts at:      0     575     639 
   Size:        575      64
 
SacII CC_GC'GG

Cuts at:      0       7     639 
   Size:          7     632
 
SfcI C'TryA_G

Cuts at:      0     222     639 
   Size:        222     417
 
SmaI CCC'GGG

Cuts at:      0     268     639 
   Size:        268     371
 
SmlI C'TyrA_G

Cuts at:      0     471     639 
   Size:        471     168
 
StuI AGG'CCT

Cuts at:      0     202     639 
   Size:        202     437
 
StyI C'CwwG_G

Cuts at:      0     372     639 
   Size:        372     267
 
TatI w'GTAC_w

Cuts at:      0     547     639 
   Size:        547      92
 
XmaI C'CCGG_G

Cuts at:      0     266     639 
   Size:        266     373
 

 Enzymes that do cut:

     AccI    AflII     ApoI     AvaI     BbsI     BcgI     BmrI     BpmI
    BseRI     BsmI    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstBI
     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI     FalI     MslI
   MspA1I     NspI    SacII     SfcI     SmaI     SmlI     StuI     StyI
     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI   BssHII   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI