(Linear) (Six-base) MAPSORT of: KIEE6527.seq  Check: 885  from: 1  to: 522



KIEE6527.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     522 
   Size:        319     203
 
AlwNI CAG_nnn'CTG

Cuts at:      0     122     432     522 
   Size:        122     310      90

  Fragments arranged by size: 

                 310     122      90
 
AvaI C'yCGr_G

Cuts at:      0     153     522 
   Size:        153     369
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     412     445     522 
   Size:        412      33      77

  Fragments arranged by size: 

                 412      77      33
 
BanII G_rGCy'C

Cuts at:      0     388     496     522 
   Size:        388     108      26

  Fragments arranged by size: 

                 388     108      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     325     522 
   Size:        325     197
 
BbvCI CC'TCA_GC

Cuts at:      0     214     522 
   Size:        214     308
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     477     522 
   Size:        477      45
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342     522 
   Size:        342     180
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     400     522 
   Size:        145     255     122

  Fragments arranged by size: 

                 255     145     122
 
Bpu10I CC'TnA_GC

Cuts at:      0     214     522 
   Size:        214     308
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     522 
   Size:        108     414
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     516     522 
   Size:        516       6
 
BsmI GAATG_Cn'

Cuts at:      0      58     486     522 
   Size:         58     428      36

  Fragments arranged by size: 

                 428      58      36
 
Bsp1286I G_dGCh'C

Cuts at:      0     388     496     522 
   Size:        388     108      26

  Fragments arranged by size: 

                 388     108      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     440     522 
   Size:         83     357      82

  Fragments arranged by size: 

                 357      83      82
 
BsrBI CCG'CTC

Cuts at:      0     230     522 
   Size:        230     292
 
BssHII G'CGCG_C

Cuts at:      0     106     522 
   Size:        106     416
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     432     522 
   Size:        432      90
 
BtgI C'CryG_G

Cuts at:      0     177     522 
   Size:        177     345
 
BtsI GCAGTG_nn'

Cuts at:      0     208     522 
   Size:        208     314
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     319     400     522 
   Size:        145     174      81     122

  Fragments arranged by size: 

                 174     145     122      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      19     522 
   Size:         19     503
 
EcoO109I rG'GnC_Cy

Cuts at:      0     393     522 
   Size:        393     129
 
HaeII r_GCGC'y

Cuts at:      0     459     522 
   Size:        459      63
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     522 
   Size:        434      88
 
MspA1I CmG'CkG

Cuts at:      0      95     119     179     522 
   Size:         95      24      60     343

  Fragments arranged by size: 

                 343      95      60      24
 
PpuMI rG'GwC_Cy

Cuts at:      0     393     522 
   Size:        393     129
 
PstI C_TGCA'G

Cuts at:      0      80     522 
   Size:         80     442
 
RsrII CG'GwC_CG

Cuts at:      0     225     522 
   Size:        225     297
 
SacII CC_GC'GG

Cuts at:      0     180     522 
   Size:        180     342
 
ScaI AGT'ACT

Cuts at:      0     166     522 
   Size:        166     356
 
SfcI C'TryA_G

Cuts at:      0      76     291     450     522 
   Size:         76     215     159      72

  Fragments arranged by size: 

                 215     159      76      72
 
SmlI C'TyrA_G

Cuts at:      0      87     522 
   Size:         87     435
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     213     522 
   Size:        213     309
 
TatI w'GTAC_w

Cuts at:      0     164     522 
   Size:        164     358
 
Tth111I GACn'n_nGTC

Cuts at:      0     223     522 
   Size:        223     299
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BaeI    BanII     BbsI    BbvCI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BseRI     BsmI Bsp1286I    BspMI
    BsrBI   BssHII   BstAPI     BtgI     BtsI  Eco57MI    EcoNI EcoO109I
    HaeII     MmeI   MspA1I    PpuMI     PstI    RsrII    SacII     ScaI
     SfcI     SmlI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII     SacI     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI