(Linear) (Six-base) MAPSORT of: KIEE6523.seq  Check: 5970  from: 1  to: 282



KIEE6523.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     282 
   Size:        162     120
 
AleI CACnn'nnGTG

Cuts at:      0      57     282 
   Size:         57     225
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     282 
   Size:         87     195
 
BanI G'GyrC_C

Cuts at:      0     197     282 
   Size:        197      85
 
BanII G_rGCy'C

Cuts at:      0      76     250     282 
   Size:         76     174      32

  Fragments arranged by size: 

                 174      76      32
 
BbeI G_GCGC'C

Cuts at:      0     201     282 
   Size:        201      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      35     131     282 
   Size:         35      96     151

  Fragments arranged by size: 

                 151      96      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     160     282 
   Size:        160     122
 
BsaHI Gr'CG_yC

Cuts at:      0     198     282 
   Size:        198      84
 
BseYI C'CCAG_C

Cuts at:      0      75     282 
   Size:         75     207
 
BsiHKAI G_wGCw'C

Cuts at:      0     114     282 
   Size:        114     168
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     114     250     282 
   Size:         76      38     136      32

  Fragments arranged by size: 

                 136      76      38      32
 
BsrFI r'CCGG_y

Cuts at:      0     194     282 
   Size:        194      88
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     268     282 
   Size:        268      14
 
BstYI r'GATC_y

Cuts at:      0     205     282 
   Size:        205      77
 
BtgI C'CryG_G

Cuts at:      0     255     261     282 
   Size:        255       6      21

  Fragments arranged by size: 

                 255      21       6
 
BtsI GCAGTG_nn'

Cuts at:      0      31     234     282 
   Size:         31     203      48

  Fragments arranged by size: 

                 203      48      31
 
EaeI y'GGCC_r

Cuts at:      0     258     282 
   Size:        258      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     105     282 
   Size:        105     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     282 
   Size:        162     120
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     219     282 
   Size:        219      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     182     282 
   Size:        182     100
 
HaeII r_GCGC'y

Cuts at:      0     201     282 
   Size:        201      81
 
KasI G'GCGC_C

Cuts at:      0     197     282 
   Size:        197      85
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     282 
   Size:        233      49
 
MscI TGG'CCA

Cuts at:      0     260     282 
   Size:        260      22
 
MslI CAynn'nnrTG

Cuts at:      0      57     282 
   Size:         57     225
 
NaeI GCC'GGC

Cuts at:      0     196     282 
   Size:        196      86
 
NarI GG'CG_CC

Cuts at:      0     198     282 
   Size:        198      84
 
NcoI C'CATG_G

Cuts at:      0     261     282 
   Size:        261      21
 
NgoMIV G'CCGG_C

Cuts at:      0     194     282 
   Size:        194      88
 
PpuMI rG'GwC_Cy

Cuts at:      0     182     282 
   Size:        182     100
 
SfoI GGC'GCC

Cuts at:      0     199     282 
   Size:        199      83
 
StyI C'CwwG_G

Cuts at:      0     261     282 
   Size:        261      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      69     282 
   Size:         69     213
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     166     198     282 
   Size:        166      32      84

  Fragments arranged by size: 

                 166      84      32
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     BanI    BanII     BbeI     BmrI   Bpu10I
    BsaHI    BseYI  BsiHKAI Bsp1286I    BsrFI    BstXI    BstYI     BtgI
     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    HaeII     KasI
     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV    PpuMI
     SfoI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI   MspA1I     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI