Restriction map for
pFN21AE6519
(Linear) (Six-base) MAPSORT of: KIEE6519.seq Check: 2302 from: 1 to: 639
KIEE6519.orf
With 182 enzymes: SgfI *
March 16, 2015 11:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 181 354 639
Size: 181 173 285
Fragments arranged by size:
285 181 173
Acc65I G'GTAC_C
Cuts at: 0 217 639
Size: 217 422
AleI CACnn'nnGTG
Cuts at: 0 490 559 639
Size: 490 69 80
Fragments arranged by size:
490 80 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 639
Size: 535 32 72
Fragments arranged by size:
535 72 32
AlwNI CAG_nnn'CTG
Cuts at: 0 456 639
Size: 456 183
ApoI r'AATT_y
Cuts at: 0 120 279 639
Size: 120 159 360
Fragments arranged by size:
360 159 120
BanI G'GyrC_C
Cuts at: 0 217 639
Size: 217 422
BanII G_rGCy'C
Cuts at: 0 78 639
Size: 78 561
BbsI GAAGACnn'nnnn_
Cuts at: 0 356 639
Size: 356 283
BmrI ACTGGGnnnn_n'
Cuts at: 0 549 639
Size: 549 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 582 639
Size: 168 414 57
Fragments arranged by size:
414 168 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 161 352 639
Size: 36 125 191 287
Fragments arranged by size:
287 191 125 36
BsaWI w'CCGG_w
Cuts at: 0 324 639
Size: 324 315
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 472 639
Size: 40 432 167
Fragments arranged by size:
432 167 40
BseYI C'CCAG_C
Cuts at: 0 185 379 533 639
Size: 185 194 154 106
Fragments arranged by size:
194 185 154 106
BsiHKAI G_wGCw'C
Cuts at: 0 78 497 509 639
Size: 78 419 12 130
Fragments arranged by size:
419 130 78 12
Bsp1286I G_dGCh'C
Cuts at: 0 78 497 509 639
Size: 78 419 12 130
Fragments arranged by size:
419 130 78 12
BspEI T'CCGG_A
Cuts at: 0 324 639
Size: 324 315
BspHI T'CATG_A
Cuts at: 0 431 639
Size: 431 208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 181 354 639
Size: 181 173 285
Fragments arranged by size:
285 181 173
BstBI TT'CG_AA
Cuts at: 0 211 639
Size: 211 428
BstYI r'GATC_y
Cuts at: 0 243 327 639
Size: 243 84 312
Fragments arranged by size:
312 243 84
BtsI GCAGTG_nn'
Cuts at: 0 592 639
Size: 592 47
DraIII CAC_nnn'GTG
Cuts at: 0 584 639
Size: 584 55
EarI CTCTTCn'nnn_
Cuts at: 0 296 639
Size: 296 343
EcoICRI GAG'CTC
Cuts at: 0 76 639
Size: 76 563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 161 352 639
Size: 36 125 191 287
Fragments arranged by size:
287 191 125 36
EcoO109I rG'GnC_Cy
Cuts at: 0 573 588 612 639
Size: 573 15 24 27
Fragments arranged by size:
573 27 24 15
EcoRI G'AATT_C
Cuts at: 0 120 639
Size: 120 519
KpnI G_GTAC'C
Cuts at: 0 221 639
Size: 221 418
MslI CAynn'nnrTG
Cuts at: 0 110 490 559 639
Size: 110 380 69 80
Fragments arranged by size:
380 110 80 69
MspA1I CmG'CkG
Cuts at: 0 240 639
Size: 240 399
NspI r_CATG'y
Cuts at: 0 257 639
Size: 257 382
PfoI T'CCnGG_A
Cuts at: 0 515 639
Size: 515 124
PpuMI rG'GwC_Cy
Cuts at: 0 573 588 612 639
Size: 573 15 24 27
Fragments arranged by size:
573 27 24 15
PstI C_TGCA'G
Cuts at: 0 599 639
Size: 599 40
PvuII CAG'CTG
Cuts at: 0 240 639
Size: 240 399
SacI G_AGCT'C
Cuts at: 0 78 639
Size: 78 561
SanDI GG'GwC_CC
Cuts at: 0 588 639
Size: 588 51
ScaI AGT'ACT
Cuts at: 0 439 639
Size: 439 200
SfcI C'TryA_G
Cuts at: 0 36 595 639
Size: 36 559 44
Fragments arranged by size:
559 44 36
SphI G_CATG'C
Cuts at: 0 257 639
Size: 257 382
TatI w'GTAC_w
Cuts at: 0 339 437 639
Size: 339 98 202
Fragments arranged by size:
339 202 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 453 639
Size: 60 393 186
Fragments arranged by size:
393 186 60
Enzymes that do cut:
AarI Acc65I AleI AloI AlwNI ApoI BanI BanII
BbsI BmrI BplI BpmI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspEI BspHI BspMI BstBI BstYI BtsI DraIII
EarI EcoICRI Eco57MI EcoO109I EcoRI KpnI MslI MspA1I
NspI PfoI PpuMI PstI PvuII SacI SanDI ScaI
SfcI SphI TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI