(Linear) (Six-base) MAPSORT of: KIEE6517.seq  Check: 8955  from: 1  to: 636



KIEE6517.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     557     590     636 
   Size:         68     489      33      46

  Fragments arranged by size: 

                 489      68      46      33
 
AflII C'TTAA_G

Cuts at:      0      69     636 
   Size:         69     567
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445     636 
   Size:        445     191
 
ApoI r'AATT_y

Cuts at:      0     116     503     636 
   Size:        116     387     133

  Fragments arranged by size: 

                 387     133     116
 
AseI AT'TA_AT

Cuts at:      0     239     636 
   Size:        239     397
 
BanII G_rGCy'C

Cuts at:      0     203     636 
   Size:        203     433
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     523     581     636 
   Size:        523      58      55

  Fragments arranged by size: 

                 523      58      55
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     315     636 
   Size:        315     321
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     334     364     636 
   Size:        334      30     272

  Fragments arranged by size: 

                 334     272      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     566     636 
   Size:        566      70
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     566     636 
   Size:        203     363      70

  Fragments arranged by size: 

                 363     203      70
 
BsrBI CCG'CTC

Cuts at:      0       5     636 
   Size:          5     631
 
BsrDI GCAATG_nn'

Cuts at:      0     383     636 
   Size:        383     253
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     166     243     636 
   Size:        166      77     393

  Fragments arranged by size: 

                 393     166      77
 
Bsu36I CC'TnA_GG

Cuts at:      0      31     636 
   Size:         31     605
 
BtgI C'CryG_G

Cuts at:      0     159     636 
   Size:        159     477
 
DraI TTT'AAA

Cuts at:      0     272     636 
   Size:        272     364
 
EaeI y'GGCC_r

Cuts at:      0     623     636 
   Size:        623      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     191     636 
   Size:        191     445
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     557     590     636 
   Size:         68     489      33      46

  Fragments arranged by size: 

                 489      68      46      33
 
EcoRI G'AATT_C

Cuts at:      0     116     636 
   Size:        116     520
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      75     636 
   Size:         75     561
 
MscI TGG'CCA

Cuts at:      0     625     636 
   Size:        625      11
 
MslI CAynn'nnrTG

Cuts at:      0     397     636 
   Size:        397     239
 
MspA1I CmG'CkG

Cuts at:      0     479     636 
   Size:        479     157
 
NdeI CA'TA_TG

Cuts at:      0     291     636 
   Size:        291     345
 
PacI TTA_AT'TAA

Cuts at:      0     188     636 
   Size:        188     448
 
PfoI T'CCnGG_A

Cuts at:      0     434     636 
   Size:        434     202
 
SapI GCTCTTCn'nnn_

Cuts at:      0     191     636 
   Size:        191     445
 
SfcI C'TryA_G

Cuts at:      0     107     636 
   Size:        107     529
 
SmlI C'TyrA_G

Cuts at:      0      69     636 
   Size:         69     567
 
SspI AAT'ATT

Cuts at:      0     276     636 
   Size:        276     360
 
StyI C'CwwG_G

Cuts at:      0      11     417     636 
   Size:         11     406     219

  Fragments arranged by size: 

                 406     219      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     172     469     636 
   Size:        172     297     167

  Fragments arranged by size: 

                 297     172     167
 
TatI w'GTAC_w

Cuts at:      0     173     636 
   Size:        173     463
 

 Enzymes that do cut:

     AcuI    AflII     AlfI     ApoI     AseI    BanII     BbsI     BmrI
    BsaXI  BsiHKAI Bsp1286I    BsrBI    BsrDI    BstXI   Bsu36I     BtgI
     DraI     EaeI     EarI  Eco57MI    EcoRI     FalI     MscI     MslI
   MspA1I     NdeI     PacI     PfoI     SapI     SfcI     SmlI     SspI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI