(Linear) (Six-base) MAPSORT of: KIEE6513.seq  Check: 3016  from: 1  to: 417



KIEE6513.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      99     417 
   Size:         99     318
 
ApaLI G'TGCA_C

Cuts at:      0     166     417 
   Size:        166     251
 
Bme1580I G_kGCm'C

Cuts at:      0     170     245     417 
   Size:        170      75     172

  Fragments arranged by size: 

                 172     170      75
 
BsaAI yAC'GTr

Cuts at:      0     240     282     417 
   Size:        240      42     135

  Fragments arranged by size: 

                 240     135      42
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     417 
   Size:        238     179
 
BsiHKAI G_wGCw'C

Cuts at:      0     170     417 
   Size:        170     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     245     417 
   Size:        170      75     172

  Fragments arranged by size: 

                 172     170      75
 
BspHI T'CATG_A

Cuts at:      0     188     417 
   Size:        188     229
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      99     417 
   Size:         99     318
 
EcoRV GAT'ATC

Cuts at:      0     387     417 
   Size:        387      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     417 
   Size:        200     217
 
MslI CAynn'nnrTG

Cuts at:      0     127     290     417 
   Size:        127     163     127

  Fragments arranged by size: 

                 163     127     127
 
MspA1I CmG'CkG

Cuts at:      0     105     417 
   Size:        105     312
 
PstI C_TGCA'G

Cuts at:      0     110     417 
   Size:        110     307
 
PvuII CAG'CTG

Cuts at:      0     105     417 
   Size:        105     312
 
SfcI C'TryA_G

Cuts at:      0     106     417 
   Size:        106     311
 
SnaBI TAC'GTA

Cuts at:      0     282     417 
   Size:        282     135
 

 Enzymes that do cut:

     AarI    ApaLI Bme1580I    BsaAI    BseRI  BsiHKAI Bsp1286I    BspHI
    BspMI    EcoRV     MmeI     MslI   MspA1I     PstI    PvuII     SfcI
    SnaBI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI