(Linear) (Six-base) MAPSORT of: KIEE6511.seq  Check: 2571  from: 1  to: 525



KIEE6511.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     470     525 
   Size:        470      55
 
AlwNI CAG_nnn'CTG

Cuts at:      0     402     457     525 
   Size:        402      55      68

  Fragments arranged by size: 

                 402      68      55
 
ApaLI G'TGCA_C

Cuts at:      0      10     469     525 
   Size:         10     459      56

  Fragments arranged by size: 

                 459      56      10
 
AvaI C'yCGr_G

Cuts at:      0     306     525 
   Size:        306     219
 
BanI G'GyrC_C

Cuts at:      0     310     463     525 
   Size:        310     153      62

  Fragments arranged by size: 

                 310     153      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     199     525 
   Size:        199     326
 
BbvCI CC'TCA_GC

Cuts at:      0       5     525 
   Size:          5     520
 
Bme1580I G_kGCm'C

Cuts at:      0      14     299     313     473     525 
   Size:         14     285      14     160      52

  Fragments arranged by size: 

                 285     160      52      14      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     293     525 
   Size:        293     232
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     525 
   Size:        496      29
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     525 
   Size:          5     520
 
BsaAI yAC'GTr

Cuts at:      0      15     335     525 
   Size:         15     320     190

  Fragments arranged by size: 

                 320     190      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     430     525 
   Size:        172     258      95

  Fragments arranged by size: 

                 258     172      95
 
BsiEI CG_ry'CG

Cuts at:      0     435     507     525 
   Size:        435      72      18

  Fragments arranged by size: 

                 435      72      18
 
BsiHKAI G_wGCw'C

Cuts at:      0      14     473     525 
   Size:         14     459      52

  Fragments arranged by size: 

                 459      52      14
 
BsmI GAATG_Cn'

Cuts at:      0      77     525 
   Size:         77     448
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     299     313     473     525 
   Size:         14     285      14     160      52

  Fragments arranged by size: 

                 285     160      52      14      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     194     525 
   Size:        194     331
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     402     525 
   Size:        402     123
 
EaeI y'GGCC_r

Cuts at:      0     258     525 
   Size:        258     267
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     525 
   Size:         67     458
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     525 
   Size:        496      29
 
MspA1I CmG'CkG

Cuts at:      0      41     263     321     399     454     525 
   Size:         41     222      58      78      55      71

  Fragments arranged by size: 

                 222      78      71      58      55      41
 
PasI CC'CwG_GG

Cuts at:      0     345     525 
   Size:        345     180
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     321     525 
   Size:        321     204
 
PmlI CAC'GTG

Cuts at:      0      15     335     525 
   Size:         15     320     190

  Fragments arranged by size: 

                 320     190      15
 
PvuII CAG'CTG

Cuts at:      0     321     399     454     525 
   Size:        321      78      55      71

  Fragments arranged by size: 

                 321      78      71      55
 
SfcI C'TryA_G

Cuts at:      0     420     525 
   Size:        420     105
 
SmaI CCC'GGG

Cuts at:      0     308     525 
   Size:        308     217
 
SrfI GCCC'GGGC

Cuts at:      0     308     525 
   Size:        308     217
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     328     373     525 
   Size:        328      45     152

  Fragments arranged by size: 

                 328     152      45
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     253     525 
   Size:        253     272
 
XmaI C'CCGG_G

Cuts at:      0     306     525 
   Size:        306     219
 

 Enzymes that do cut:

     AlfI    AlwNI    ApaLI     AvaI     BanI     BbsI    BbvCI Bme1580I
     BmrI     BpmI   Bpu10I    BsaAI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspMI   BstAPI     EaeI     EarI  Eco57MI   MspA1I     PasI
    PflMI     PmlI    PvuII     SfcI     SmaI     SrfI    TaqII     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI