(Linear) (Six-base) MAPSORT of: KIEE6508.seq  Check: 2333  from: 1  to: 525



KIEE6508.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     525 
   Size:        366     159
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     274     525 
   Size:        274     251
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61     525 
   Size:         61     464
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     497     525 
   Size:        497      28
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     525 
   Size:        525
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     525 
   Size:        287     238
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     525 
   Size:        125     400
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      15     525 
   Size:         15     510
 
BstYI r'GATC_y

Cuts at:      0     233     525 
   Size:        233     292
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     330     525 
   Size:        330     195
 
BtsI GCAGTG_nn'

Cuts at:      0      62     525 
   Size:         62     463
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0       2      37     525 
   Size:          2      35     488

  Fragments arranged by size: 

                 488      35       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     437     525 
   Size:        200     237      88

  Fragments arranged by size: 

                 237     200      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      60     525 
   Size:         60     465
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     366     525 
   Size:        287      79     159

  Fragments arranged by size: 

                 287     159      79
 
EcoRV GAT'ATC

Cuts at:      0     180     525 
   Size:        180     345
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     346     378     525 
   Size:        346      32     147

  Fragments arranged by size: 

                 346     147      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     389     525 
   Size:        115     274     136

  Fragments arranged by size: 

                 274     136     115
 
NspI r_CATG'y

Cuts at:      0     261     525 
   Size:        261     264
 
SapI GCTCTTCn'nnn_

Cuts at:      0     200     525 
   Size:        200     325
 
ScaI AGT'ACT

Cuts at:      0     387     525 
   Size:        387     138
 
SexAI A'CCwGG_T

Cuts at:      0      94     525 
   Size:         94     431
 
SfcI C'TryA_G

Cuts at:      0      62     525 
   Size:         62     463
 
SmlI C'TyrA_G

Cuts at:      0     140     525 
   Size:        140     385
 
TatI w'GTAC_w

Cuts at:      0     385     525 
   Size:        385     140
 
Tth111I GACn'n_nGTC

Cuts at:      0      96     525 
   Size:         96     429
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     BaeI    BciVI     BpmI    BpuEI     BsaI
    BstYI    BtgZI     BtsI    CspCI     EarI     EciI  Eco57MI    EcoRV
    Hin4I     MmeI     NspI     SapI     ScaI    SexAI     SfcI     SmlI
     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI