(Linear) (Six-base) MAPSORT of: KIEE6506.seq  Check: 3807  from: 1  to: 312



KIEE6506.orf

 With 182 enzymes: SgfI * 

                        November 17, 2014 16:44  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     159     312 
   Size:        159     153
 
ApoI r'AATT_y

Cuts at:      0      40     312 
   Size:         40     272
 
Bme1580I G_kGCm'C

Cuts at:      0     311     312 
   Size:        311       1
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     310     312 
   Size:        256      54       2

  Fragments arranged by size: 

                 256      54       2
 
Bpu10I CC'TnA_GC

Cuts at:      0      35     312 
   Size:         35     277
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      61     312 
   Size:         61     251
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      79     109     312 
   Size:         79      30     203

  Fragments arranged by size: 

                 203      79      30
 
BseYI C'CCAG_C

Cuts at:      0     152     312 
   Size:        152     160
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     109     312 
   Size:        109     203
 
Bsp1286I G_dGCh'C

Cuts at:      0     311     312 
   Size:        311       1
 
BsrDI GCAATG_nn'

Cuts at:      0     228     312 
   Size:        228      84
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      31     312 
   Size:         31     281
 
BstEII G'GTnAC_C

Cuts at:      0     147     312 
   Size:        147     165
 
BtgI C'CryG_G

Cuts at:      0      98     312 
   Size:         98     214
 
EaeI y'GGCC_r

Cuts at:      0     272     312 
   Size:        272      40
 
EarI CTCTTCn'nnn_

Cuts at:      0     188     312 
   Size:        188     124
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     310     312 
   Size:        256      54       2

  Fragments arranged by size: 

                 256      54       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     141     312 
   Size:        141     171
 
EcoRI G'AATT_C

Cuts at:      0      40     312 
   Size:         40     272
 
MspA1I CmG'CkG

Cuts at:      0     159     218     312 
   Size:        159      59      94

  Fragments arranged by size: 

                 159      94      59
 
PpuMI rG'GwC_Cy

Cuts at:      0     141     312 
   Size:        141     171
 
PvuII CAG'CTG

Cuts at:      0     159     218     312 
   Size:        159      59      94

  Fragments arranged by size: 

                 159      94      59
 
ScaI AGT'ACT

Cuts at:      0      94     312 
   Size:         94     218
 
SexAI A'CCwGG_T

Cuts at:      0     143     312 
   Size:        143     169
 
StuI AGG'CCT

Cuts at:      0     297     312 
   Size:        297      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       2     166     312 
   Size:          2     164     146

  Fragments arranged by size: 

                 164     146       2
 
TatI w'GTAC_w

Cuts at:      0      92     312 
   Size:         92     220
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      79     111     312 
   Size:         79      32     201

  Fragments arranged by size: 

                 201      79      32
 
Tth111I GACn'n_nGTC

Cuts at:      0       8     145     312 
   Size:          8     137     167

  Fragments arranged by size: 

                 167     137       8
 

 Enzymes that do cut:

    AlwNI     ApoI Bme1580I     BpmI   Bpu10I     BsaI    BsaXI    BseYI
    BsmBI Bsp1286I    BsrDI   BstAPI   BstEII     BtgI     EaeI     EarI
  Eco57MI EcoO109I    EcoRI   MspA1I    PpuMI    PvuII     ScaI    SexAI
     StuI    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI