(Linear) (Six-base) MAPSORT of: KIEE6503.seq  Check: 2839  from: 1  to: 459



KIEE6503.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AleI CACnn'nnGTG

Cuts at:      0      43     459 
   Size:         43     416
 
BlpI GC'TnA_GC

Cuts at:      0     112     459 
   Size:        112     347
 
Bme1580I G_kGCm'C

Cuts at:      0      13     459 
   Size:         13     446
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     459 
   Size:        264     195
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      97     211     459 
   Size:         97     114     248

  Fragments arranged by size: 

                 248     114      97
 
BsaAI yAC'GTr

Cuts at:      0      78     459 
   Size:         78     381
 
BsaWI w'CCGG_w

Cuts at:      0     434     459 
   Size:        434      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     459 
   Size:         85     374
 
BseYI C'CCAG_C

Cuts at:      0     419     459 
   Size:        419      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     459 
   Size:        175     284
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     459 
   Size:        377      82
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     377     459 
   Size:         13     364      82

  Fragments arranged by size: 

                 364      82      13
 
BspEI T'CCGG_A

Cuts at:      0     434     459 
   Size:        434      25
 
BstYI r'GATC_y

Cuts at:      0     362     459 
   Size:        362      97
 
BtgI C'CryG_G

Cuts at:      0      57     426     459 
   Size:         57     369      33

  Fragments arranged by size: 

                 369      57      33
 
DraIII CAC_nnn'GTG

Cuts at:      0     105     198     459 
   Size:        105      93     261

  Fragments arranged by size: 

                 261     105      93
 
EaeI y'GGCC_r

Cuts at:      0      83     459 
   Size:         83     376
 
MscI TGG'CCA

Cuts at:      0      85     459 
   Size:         85     374
 
MslI CAynn'nnrTG

Cuts at:      0      43     175     459 
   Size:         43     132     284

  Fragments arranged by size: 

                 284     132      43
 
NspI r_CATG'y

Cuts at:      0     180     459 
   Size:        180     279
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79     330     459 
   Size:         79     251     129

  Fragments arranged by size: 

                 251     129      79
 
TatI w'GTAC_w

Cuts at:      0     326     459 
   Size:        326     133
 

 Enzymes that do cut:

     AleI     BlpI Bme1580I     BmrI     BsaI    BsaAI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BstYI     BtgI   DraIII
     EaeI     MscI     MslI     NspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI