Restriction map for
pFN21AE6496
(Linear) (Six-base) MAPSORT of: KIEE6496.seq Check: 881 from: 1 to: 1596
KIEE6496.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1070 1596
Size: 1070 526
AccI GT'mk_AC
Cuts at: 0 1025 1190 1259 1596
Size: 1025 165 69 337
Fragments arranged by size:
1025 337 165 69
AflIII A'CryG_T
Cuts at: 0 578 1596
Size: 578 1018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 742 1596
Size: 742 854
AlwNI CAG_nnn'CTG
Cuts at: 0 558 1071 1596
Size: 558 513 525
Fragments arranged by size:
558 525 513
ApaI G_GGCC'C
Cuts at: 0 211 431 1307 1596
Size: 211 220 876 289
Fragments arranged by size:
876 289 220 211
ApaLI G'TGCA_C
Cuts at: 0 635 1327 1596
Size: 635 692 269
Fragments arranged by size:
692 635 269
AvaI C'yCGr_G
Cuts at: 0 29 223 815 1300 1596
Size: 29 194 592 485 296
Fragments arranged by size:
592 485 296 194 29
BanI G'GyrC_C
Cuts at: 0 95 1102 1596
Size: 95 1007 494
Fragments arranged by size:
1007 494 95
BanII G_rGCy'C
Cuts at: 0 83 211 239 325 431 565 816 1307
Size: 83 128 28 86 106 134 251 491
Cuts at: 1307 1596
Size: 289
Fragments arranged by size:
491 289 251 134 128 106 86 83
28
BbeI G_GCGC'C
Cuts at: 0 99 1106 1596
Size: 99 1007 490
Fragments arranged by size:
1007 490 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 1596
Size: 365 1231
BglI GCCn_nnn'nGGC
Cuts at: 0 34 87 280 554 880 1523 1544 1596
Size: 34 53 193 274 326 643 21 52
Fragments arranged by size:
643 326 274 193 53 52 34 21
BlpI GC'TnA_GC
Cuts at: 0 324 564 1351 1596
Size: 324 240 787 245
Fragments arranged by size:
787 324 245 240
Bme1580I G_kGCm'C
Cuts at: 0 164 173 211 431 639 731 838 1124
Size: 164 9 38 220 208 92 107 286
Cuts at: 1124 1307 1331 1596
Size: 183 24 265
Fragments arranged by size:
286 265 220 208 183 164 107 92
38 24 9
BmgBI CAC'GTC
Cuts at: 0 363 456 581 1230 1596
Size: 363 93 125 649 366
Fragments arranged by size:
649 366 363 125 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 649 1596
Size: 649 947
BmtI G_CTAG'C
Cuts at: 0 745 1596
Size: 745 851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1596
Size: 441 1155
BsaI GGTCTCn'nnnn_
Cuts at: 0 1158 1596
Size: 1158 438
BsaAI yAC'GTr
Cuts at: 0 941 1596
Size: 941 655
BsaHI Gr'CG_yC
Cuts at: 0 96 295 479 1022 1103 1596
Size: 96 199 184 543 81 493
Fragments arranged by size:
543 493 199 184 96 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 204 234 1596
Size: 204 30 1362
Fragments arranged by size:
1362 204 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 1596
Size: 631 965
BseYI C'CCAG_C
Cuts at: 0 1017 1335 1596
Size: 1017 318 261
Fragments arranged by size:
1017 318 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1596
Size: 791 805
BsiEI CG_ry'CG
Cuts at: 0 441 1596
Size: 441 1155
BsiHKAI G_wGCw'C
Cuts at: 0 639 1331 1596
Size: 639 692 265
Fragments arranged by size:
692 639 265
Bsp1286I G_dGCh'C
Cuts at: 0 83 164 173 211 239 325 431 565
Size: 83 81 9 38 28 86 106 134
Cuts at: 565 639 731 816 838 1124 1307 1331 1596
Size: 74 92 85 22 286 183 24 265
Fragments arranged by size:
286 265 183 134 106 92 86 85
83 81 74 38 28 24 22 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 833 1070 1385 1596
Size: 24 809 237 315 211
Fragments arranged by size:
809 315 237 211 24
BsrBI CCG'CTC
Cuts at: 0 425 1133 1596
Size: 425 708 463
Fragments arranged by size:
708 463 425
BsrDI GCAATG_nn'
Cuts at: 0 869 932 1596
Size: 869 63 664
Fragments arranged by size:
869 664 63
BsrFI r'CCGG_y
Cuts at: 0 715 883 996 1596
Size: 715 168 113 600
Fragments arranged by size:
715 600 168 113
BsrGI T'GTAC_A
Cuts at: 0 146 1596
Size: 146 1450
BssHII G'CGCG_C
Cuts at: 0 541 887 1048 1079 1596
Size: 541 346 161 31 517
Fragments arranged by size:
541 517 346 161 31
BssSI C'ACGA_G
Cuts at: 0 1110 1596
Size: 1110 486
BtgI C'CryG_G
Cuts at: 0 8 875 1596
Size: 8 867 721
Fragments arranged by size:
867 721 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 165 1251 1460 1596
Size: 165 1086 209 136
Fragments arranged by size:
1086 209 165 136
BtsI GCAGTG_nn'
Cuts at: 0 788 1072 1198 1596
Size: 788 284 126 398
Fragments arranged by size:
788 398 284 126
DraIII CAC_nnn'GTG
Cuts at: 0 1326 1596
Size: 1326 270
EaeI y'GGCC_r
Cuts at: 0 38 260 281 313 785 881 1370 1515
Size: 38 222 21 32 472 96 489 145
Cuts at: 1515 1596
Size: 81
Fragments arranged by size:
489 472 222 145 96 81 38 32
21
EcoO109I rG'GnC_Cy
Cuts at: 0 207 208 428 555 1596
Size: 207 1 220 127 1041
Fragments arranged by size:
1041 220 207 127 1
HaeII r_GCGC'y
Cuts at: 0 73 99 127 275 652 1106 1596
Size: 73 26 28 148 377 454 490
Fragments arranged by size:
490 454 377 148 73 28 26
HincII GTy'rAC
Cuts at: 0 1191 1596
Size: 1191 405
KasI G'GCGC_C
Cuts at: 0 95 1102 1596
Size: 95 1007 494
Fragments arranged by size:
1007 494 95
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1596
Size: 32 1564
MscI TGG'CCA
Cuts at: 0 315 787 1372 1517 1596
Size: 315 472 585 145 79
Fragments arranged by size:
585 472 315 145 79
MslI CAynn'nnrTG
Cuts at: 0 592 874 943 1596
Size: 592 282 69 653
Fragments arranged by size:
653 592 282 69
MspA1I CmG'CkG
Cuts at: 0 10 249 307 319 405 462 1596
Size: 10 239 58 12 86 57 1134
Fragments arranged by size:
1134 239 86 58 57 12 10
NaeI GCC'GGC
Cuts at: 0 717 885 998 1596
Size: 717 168 113 598
Fragments arranged by size:
717 598 168 113
NarI GG'CG_CC
Cuts at: 0 96 1103 1596
Size: 96 1007 493
Fragments arranged by size:
1007 493 96
NgoMIV G'CCGG_C
Cuts at: 0 715 883 996 1596
Size: 715 168 113 600
Fragments arranged by size:
715 600 168 113
NheI G'CTAG_C
Cuts at: 0 741 1596
Size: 741 855
NspI r_CATG'y
Cuts at: 0 144 192 1596
Size: 144 48 1404
Fragments arranged by size:
1404 144 48
PmlI CAC'GTG
Cuts at: 0 941 1596
Size: 941 655
PspOMI G'GGCC_C
Cuts at: 0 207 427 1303 1596
Size: 207 220 876 293
Fragments arranged by size:
876 293 220 207
PstI C_TGCA'G
Cuts at: 0 77 404 554 1067 1596
Size: 77 327 150 513 529
Fragments arranged by size:
529 513 327 150 77
PvuII CAG'CTG
Cuts at: 0 307 405 1596
Size: 307 98 1191
Fragments arranged by size:
1191 307 98
SacII CC_GC'GG
Cuts at: 0 11 1596
Size: 11 1585
SalI G'TCGA_C
Cuts at: 0 1189 1596
Size: 1189 407
SbfI CC_TGCA'GG
Cuts at: 0 554 1596
Size: 554 1042
SfcI C'TryA_G
Cuts at: 0 73 400 494 550 1063 1596
Size: 73 327 94 56 513 533
Fragments arranged by size:
533 513 327 94 73 56
SfoI GGC'GCC
Cuts at: 0 97 1104 1596
Size: 97 1007 492
Fragments arranged by size:
1007 492 97
SmaI CCC'GGG
Cuts at: 0 31 1596
Size: 31 1565
SmlI C'TyrA_G
Cuts at: 0 420 1596
Size: 420 1176
SrfI GCCC'GGGC
Cuts at: 0 31 1596
Size: 31 1565
StyI C'CwwG_G
Cuts at: 0 389 1356 1596
Size: 389 967 240
Fragments arranged by size:
967 389 240
TatI w'GTAC_w
Cuts at: 0 146 1596
Size: 146 1450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 204 236 662 694 1596
Size: 204 32 426 32 902
Fragments arranged by size:
902 426 204 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 828 1064 1596
Size: 828 236 532
Fragments arranged by size:
828 532 236
XmaI C'CCGG_G
Cuts at: 0 29 1596
Size: 29 1567
Enzymes that do cut:
AarI AccI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BtgI BtgZI BtsI DraIII
EaeI EcoO109I HaeII HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PmlI PspOMI
PstI PvuII SacII SalI SbfI SfcI SfoI SmaI
SmlI SrfI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BplI
BpmI Bpu10I BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI NcoI NdeI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII
Tth111I XbaI XhoI XmnI ZraI