Restriction map for
pFN21AE6495
(Linear) (Six-base) MAPSORT of: KIEE6495.seq Check: 1116 from: 1 to: 1374
KIEE6495.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 18 177 1374
Size: 18 159 1197
Fragments arranged by size:
1197 159 18
AfeI AGC'GCT
Cuts at: 0 755 829 1175 1374
Size: 755 74 346 199
Fragments arranged by size:
755 346 199 74
AflIII A'CryG_T
Cuts at: 0 359 554 983 1374
Size: 359 195 429 391
Fragments arranged by size:
429 391 359 195
AleI CACnn'nnGTG
Cuts at: 0 955 1186 1374
Size: 955 231 188
Fragments arranged by size:
955 231 188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 342 1374
Size: 342 1032
AlwNI CAG_nnn'CTG
Cuts at: 0 21 192 1374
Size: 21 171 1182
Fragments arranged by size:
1182 171 21
BanI G'GyrC_C
Cuts at: 0 267 655 1255 1374
Size: 267 388 600 119
Fragments arranged by size:
600 388 267 119
BbeI G_GCGC'C
Cuts at: 0 271 659 1374
Size: 271 388 715
Fragments arranged by size:
715 388 271
BglI GCCn_nnn'nGGC
Cuts at: 0 418 445 610 1374
Size: 418 27 165 764
Fragments arranged by size:
764 418 165 27
Bme1580I G_kGCm'C
Cuts at: 0 896 1374
Size: 896 478
BmgBI CAC'GTC
Cuts at: 0 305 360 1374
Size: 305 55 1014
Fragments arranged by size:
1014 305 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 534 1374
Size: 534 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 310 529 604 873 1374
Size: 247 63 219 75 269 501
Fragments arranged by size:
501 269 247 219 75 63
Bpu10I CC'TnA_GC
Cuts at: 0 1270 1374
Size: 1270 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 1374
Size: 118 1256
BsaAI yAC'GTr
Cuts at: 0 444 710 1374
Size: 444 266 664
Fragments arranged by size:
664 444 266
BsaHI Gr'CG_yC
Cuts at: 0 268 515 656 808 1011 1374
Size: 268 247 141 152 203 363
Fragments arranged by size:
363 268 247 203 152 141
BseYI C'CCAG_C
Cuts at: 0 460 618 678 724 837 1374
Size: 460 158 60 46 113 537
Fragments arranged by size:
537 460 158 113 60 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1078 1374
Size: 46 1032 296
Fragments arranged by size:
1032 296 46
BsiEI CG_ry'CG
Cuts at: 0 63 204 208 338 413 476 774 884
Size: 63 141 4 130 75 63 298 110
Cuts at: 884 940 944 1187 1374
Size: 56 4 243 187
Fragments arranged by size:
298 243 187 141 130 110 75 63
63 56 4 4
BsiHKAI G_wGCw'C
Cuts at: 0 83 296 484 1374
Size: 83 213 188 890
Fragments arranged by size:
890 213 188 83
BsmBI CGTCTCn'nnnn_
Cuts at: 0 922 1374
Size: 922 452
Bsp1286I G_dGCh'C
Cuts at: 0 83 296 484 896 1374
Size: 83 213 188 412 478
Fragments arranged by size:
478 412 213 188 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 177 228 878 1374
Size: 18 159 51 650 496
Fragments arranged by size:
650 496 159 51 18
BsrBI CCG'CTC
Cuts at: 0 110 376 1374
Size: 110 266 998
Fragments arranged by size:
998 266 110
BsrDI GCAATG_nn'
Cuts at: 0 319 1374
Size: 319 1055
BsrFI r'CCGG_y
Cuts at: 0 203 207 270 760 879 886 939 1282
Size: 203 4 63 490 119 7 53 343
Cuts at: 1282 1374
Size: 92
Fragments arranged by size:
490 343 203 119 92 63 53 7
4
BssSI C'ACGA_G
Cuts at: 0 715 1374
Size: 715 659
BstAPI GCAn_nnn'nTGC
Cuts at: 0 192 1374
Size: 192 1182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 910 957 1374
Size: 910 47 417
Fragments arranged by size:
910 417 47
Bsu36I CC'TnA_GG
Cuts at: 0 428 1321 1374
Size: 428 893 53
Fragments arranged by size:
893 428 53
BtgI C'CryG_G
Cuts at: 0 821 842 956 1124 1187 1291 1374
Size: 821 21 114 168 63 104 83
Fragments arranged by size:
821 168 114 104 83 63 21
BtsI GCAGTG_nn'
Cuts at: 0 977 1029 1095 1374
Size: 977 52 66 279
Fragments arranged by size:
977 279 66 52
DraIII CAC_nnn'GTG
Cuts at: 0 530 1374
Size: 530 844
EaeI y'GGCC_r
Cuts at: 0 42 205 335 410 473 602 611 758
Size: 42 163 130 75 63 129 9 147
Cuts at: 758 771 863 881 912 937 941 998 1026
Size: 13 92 18 31 25 4 57 28
Cuts at: 1026 1121 1149 1184 1374
Size: 95 28 35 190
Fragments arranged by size:
190 163 147 130 129 95 92 75
63 57 42 35 31 28 28 25
18 13 9 4
EagI C'GGCC_G
Cuts at: 0 205 335 410 473 771 881 937 941
Size: 205 130 75 63 298 110 56 4
Cuts at: 941 1184 1374
Size: 243 190
Fragments arranged by size:
298 243 205 190 130 110 75 63
56 4
EarI CTCTTCn'nnn_
Cuts at: 0 55 109 385 542 925 1183 1374
Size: 55 54 276 157 383 258 191
Fragments arranged by size:
383 276 258 191 157 55 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 235 1374
Size: 235 1139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 310 529 604 873 1374
Size: 247 63 219 75 269 501
Fragments arranged by size:
501 269 247 219 75 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 541 1374
Size: 25 516 833
Fragments arranged by size:
833 516 25
FseI GG_CCGG'CC
Cuts at: 0 943 1374
Size: 943 431
FspI TGC'GCA
Cuts at: 0 318 1069 1374
Size: 318 751 305
Fragments arranged by size:
751 318 305
HaeII r_GCGC'y
Cuts at: 0 214 271 277 424 659 757 831 1144
Size: 214 57 6 147 235 98 74 313
Cuts at: 1144 1177 1340 1374
Size: 33 163 34
Fragments arranged by size:
313 235 214 163 147 98 74 57
34 33 6
HincII GTy'rAC
Cuts at: 0 1161 1218 1374
Size: 1161 57 156
Fragments arranged by size:
1161 156 57
HindIII A'AGCT_T
Cuts at: 0 628 1374
Size: 628 746
KasI G'GCGC_C
Cuts at: 0 267 655 1374
Size: 267 388 719
Fragments arranged by size:
719 388 267
MluI A'CGCG_T
Cuts at: 0 554 983 1374
Size: 554 429 391
Fragments arranged by size:
554 429 391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1106 1374
Size: 287 819 268
Fragments arranged by size:
819 287 268
MscI TGG'CCA
Cuts at: 0 613 1028 1123 1374
Size: 613 415 95 251
Fragments arranged by size:
613 415 251 95
MslI CAynn'nnrTG
Cuts at: 0 324 955 1186 1374
Size: 324 631 231 188
Fragments arranged by size:
631 324 231 188
MspA1I CmG'CkG
Cuts at: 0 844 1293 1374
Size: 844 449 81
Fragments arranged by size:
844 449 81
NaeI GCC'GGC
Cuts at: 0 209 272 762 881 888 941 1284 1374
Size: 209 63 490 119 7 53 343 90
Fragments arranged by size:
490 343 209 119 90 63 53 7
NarI GG'CG_CC
Cuts at: 0 268 656 1374
Size: 268 388 718
Fragments arranged by size:
718 388 268
NgoMIV G'CCGG_C
Cuts at: 0 207 270 760 879 886 939 1282 1374
Size: 207 63 490 119 7 53 343 92
Fragments arranged by size:
490 343 207 119 92 63 53 7
NotI GC'GGCC_GC
Cuts at: 0 335 1374
Size: 335 1039
NspI r_CATG'y
Cuts at: 0 120 1227 1374
Size: 120 1107 147
Fragments arranged by size:
1107 147 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1374
Size: 1035 339
PfoI T'CCnGG_A
Cuts at: 0 506 1374
Size: 506 868
PmlI CAC'GTG
Cuts at: 0 710 1374
Size: 710 664
PstI C_TGCA'G
Cuts at: 0 188 239 1374
Size: 188 51 1135
Fragments arranged by size:
1135 188 51
RsrII CG'GwC_CG
Cuts at: 0 977 1374
Size: 977 397
SacII CC_GC'GG
Cuts at: 0 845 1294 1374
Size: 845 449 80
Fragments arranged by size:
845 449 80
SapI GCTCTTCn'nnn_
Cuts at: 0 55 1183 1374
Size: 55 1128 191
Fragments arranged by size:
1128 191 55
SfcI C'TryA_G
Cuts at: 0 184 235 1374
Size: 184 51 1139
Fragments arranged by size:
1139 184 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 610 1374
Size: 610 764
SfoI GGC'GCC
Cuts at: 0 269 657 1374
Size: 269 388 717
Fragments arranged by size:
717 388 269
SgrAI Cr'CCGG_yG
Cuts at: 0 270 886 1374
Size: 270 616 488
Fragments arranged by size:
616 488 270
SphI G_CATG'C
Cuts at: 0 120 1374
Size: 120 1254
StuI AGG'CCT
Cuts at: 0 101 1374
Size: 101 1273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 994 1374
Size: 24 970 380
Fragments arranged by size:
970 380 24
Tth111I GACn'n_nGTC
Cuts at: 0 1204 1374
Size: 1204 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 597 666 819 957 1374
Size: 597 69 153 138 417
Fragments arranged by size:
597 417 153 138 69
Enzymes that do cut:
AarI AfeI AflIII AleI AlfI AlwNI BanI BbeI
BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstXI Bsu36I BtgI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
FseI FspI HaeII HincII HindIII KasI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PflMI PfoI PmlI PstI RsrII SacII SapI SfcI
SfiI SfoI SgrAI SphI StuI TaqII Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BpuEI BsaBI
BsaWI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrGI
BssHII BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DrdI EcoICRI EcoO109I EcoRI EcoRV FalI FspAI
Hin4I HpaI KpnI MfeI NcoI NdeI NheI NruI
NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SgfI SmaI SmlI SnaBI
SpeI SrfI SspI StyI SwaI TatI TstI XbaI
XhoI XmaI XmnI ZraI