Restriction map for
pFN21AE6493
(Linear) (Six-base) MAPSORT of: KIEE6493.seq Check: 4633 from: 1 to: 1830
KIEE6493.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 233 424 1830
Size: 233 191 1406
Fragments arranged by size:
1406 233 191
Acc65I G'GTAC_C
Cuts at: 0 911 1830
Size: 911 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1101 1554 1830
Size: 1101 453 276
Fragments arranged by size:
1101 453 276
AleI CACnn'nnGTG
Cuts at: 0 28 1830
Size: 28 1802
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1749 1781 1830
Size: 1749 32 49
Fragments arranged by size:
1749 49 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1090 1830
Size: 1090 740
ApoI r'AATT_y
Cuts at: 0 681 690 1461 1830
Size: 681 9 771 369
Fragments arranged by size:
771 681 369 9
AseI AT'TA_AT
Cuts at: 0 1070 1830
Size: 1070 760
BanI G'GyrC_C
Cuts at: 0 22 217 911 1830
Size: 22 195 694 919
Fragments arranged by size:
919 694 195 22
BanII G_rGCy'C
Cuts at: 0 896 1182 1380 1830
Size: 896 286 198 450
Fragments arranged by size:
896 450 286 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 1830
Size: 297 1533
BciVI GTATCCnnnnn_n'
Cuts at: 0 1601 1830
Size: 1601 229
BglII A'GATC_T
Cuts at: 0 1098 1830
Size: 1098 732
Bme1580I G_kGCm'C
Cuts at: 0 167 220 1830
Size: 167 53 1610
Fragments arranged by size:
1610 167 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 337 544 1830
Size: 337 207 1286
Fragments arranged by size:
1286 337 207
BmtI G_CTAG'C
Cuts at: 0 191 1830
Size: 191 1639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 1830
Size: 1047 783
Bpu10I CC'TnA_GC
Cuts at: 0 1077 1830
Size: 1077 753
BsaI GGTCTCn'nnnn_
Cuts at: 0 1142 1830
Size: 1142 688
BsaAI yAC'GTr
Cuts at: 0 45 1830
Size: 45 1785
BsaBI GATnn'nnATC
Cuts at: 0 600 1830
Size: 600 1230
BsaHI Gr'CG_yC
Cuts at: 0 1207 1830
Size: 1207 623
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1313 1343 1830
Size: 1313 30 487
Fragments arranged by size:
1313 487 30
BseYI C'CCAG_C
Cuts at: 0 103 1830
Size: 103 1727
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 513 811 935 1830
Size: 501 12 298 124 895
Fragments arranged by size:
895 501 298 124 12
BsiEI CG_ry'CG
Cuts at: 0 77 147 1830
Size: 77 70 1683
Fragments arranged by size:
1683 77 70
BsiHKAI G_wGCw'C
Cuts at: 0 1326 1380 1830
Size: 1326 54 450
Fragments arranged by size:
1326 450 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 388 1830
Size: 388 1442
Bsp1286I G_dGCh'C
Cuts at: 0 167 220 896 1182 1326 1380 1830
Size: 167 53 676 286 144 54 450
Fragments arranged by size:
676 450 286 167 144 54 53
BsrDI GCAATG_nn'
Cuts at: 0 1408 1414 1821 1830
Size: 1408 6 407 9
Fragments arranged by size:
1408 407 9 6
BstYI r'GATC_y
Cuts at: 0 1098 1830
Size: 1098 732
BstZ17I GTA'TAC
Cuts at: 0 425 1830
Size: 425 1405
BtgI C'CryG_G
Cuts at: 0 10 167 1830
Size: 10 157 1663
Fragments arranged by size:
1663 157 10
BtsI GCAGTG_nn'
Cuts at: 0 167 1830
Size: 167 1663
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 491 526 1830
Size: 491 35 1304
Fragments arranged by size:
1304 491 35
DraI TTT'AAA
Cuts at: 0 653 1830
Size: 653 1177
DraIII CAC_nnn'GTG
Cuts at: 0 538 1830
Size: 538 1292
EaeI y'GGCC_r
Cuts at: 0 74 111 144 1830
Size: 74 37 33 1686
Fragments arranged by size:
1686 74 37 33
EagI C'GGCC_G
Cuts at: 0 74 144 1830
Size: 74 70 1686
Fragments arranged by size:
1686 74 70
EarI CTCTTCn'nnn_
Cuts at: 0 406 1830
Size: 406 1424
EcoICRI GAG'CTC
Cuts at: 0 1378 1830
Size: 1378 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 1101 1554 1830
Size: 1047 54 453 276
Fragments arranged by size:
1047 453 276 54
EcoO109I rG'GnC_Cy
Cuts at: 0 746 1830
Size: 746 1084
EcoRV GAT'ATC
Cuts at: 0 1428 1830
Size: 1428 402
HaeII r_GCGC'y
Cuts at: 0 175 1830
Size: 175 1655
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1669 1701 1830
Size: 1669 32 129
Fragments arranged by size:
1669 129 32
HincII GTy'rAC
Cuts at: 0 777 1830
Size: 777 1053
KpnI G_GTAC'C
Cuts at: 0 915 1830
Size: 915 915
MfeI C'AATT_G
Cuts at: 0 1316 1830
Size: 1316 514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1347 1560 1830
Size: 1347 213 270
Fragments arranged by size:
1347 270 213
MslI CAynn'nnrTG
Cuts at: 0 28 920 1480 1830
Size: 28 892 560 350
Fragments arranged by size:
892 560 350 28
NheI G'CTAG_C
Cuts at: 0 187 1830
Size: 187 1643
NotI GC'GGCC_GC
Cuts at: 0 144 1830
Size: 144 1686
NspI r_CATG'y
Cuts at: 0 63 878 1720 1830
Size: 63 815 842 110
Fragments arranged by size:
842 815 110 63
PfoI T'CCnGG_A
Cuts at: 0 1002 1011 1830
Size: 1002 9 819
Fragments arranged by size:
1002 819 9
PpuMI rG'GwC_Cy
Cuts at: 0 746 1830
Size: 746 1084
SacI G_AGCT'C
Cuts at: 0 1380 1830
Size: 1380 450
SpeI A'CTAG_T
Cuts at: 0 1495 1830
Size: 1495 335
SphI G_CATG'C
Cuts at: 0 63 1830
Size: 63 1767
StyI C'CwwG_G
Cuts at: 0 125 928 1155 1431 1830
Size: 125 803 227 276 399
Fragments arranged by size:
803 399 276 227 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 526 1830
Size: 526 1304
TatI w'GTAC_w
Cuts at: 0 1565 1830
Size: 1565 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1007 1039 1151 1183 1830
Size: 1007 32 112 32 647
Fragments arranged by size:
1007 647 112 32 32
Enzymes that do cut:
AccI Acc65I AcuI AleI AloI AlwNI ApoI AseI
BanI BanII BbsI BciVI BglII Bme1580I BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BstYI BstZ17I
BtgI BtsI CspCI DraI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KpnI
MfeI MmeI MslI NheI NotI NspI PfoI PpuMI
SacI SpeI SphI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglI BlpI
BmgBI BplI BpuEI BsaWI BseRI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI Bsu36I BtgZI ClaI DrdI EciI
EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI
KasI MluI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI