Restriction map for
pFN21AE6492
(Linear) (Six-base) MAPSORT of: KIEE6492.seq Check: 3693 from: 1 to: 1833
KIEE6492.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 289 1833
Size: 289 1544
AatII G_ACGT'C
Cuts at: 0 1781 1833
Size: 1781 52
AccI GT'mk_AC
Cuts at: 0 287 377 1833
Size: 287 90 1456
Fragments arranged by size:
1456 287 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 772 1833
Size: 523 249 1061
Fragments arranged by size:
1061 523 249
AfeI AGC'GCT
Cuts at: 0 211 452 1833
Size: 211 241 1381
Fragments arranged by size:
1381 241 211
AflIII A'CryG_T
Cuts at: 0 290 1610 1833
Size: 290 1320 223
Fragments arranged by size:
1320 290 223
AleI CACnn'nnGTG
Cuts at: 0 1628 1833
Size: 1628 205
AlwNI CAG_nnn'CTG
Cuts at: 0 82 432 554 1833
Size: 82 350 122 1279
Fragments arranged by size:
1279 350 122 82
ApaLI G'TGCA_C
Cuts at: 0 666 1833
Size: 666 1167
AvaI C'yCGr_G
Cuts at: 0 566 1037 1263 1833
Size: 566 471 226 570
Fragments arranged by size:
570 566 471 226
BamHI G'GATC_C
Cuts at: 0 1112 1833
Size: 1112 721
BanI G'GyrC_C
Cuts at: 0 301 1243 1833
Size: 301 942 590
Fragments arranged by size:
942 590 301
BanII G_rGCy'C
Cuts at: 0 926 1058 1712 1833
Size: 926 132 654 121
Fragments arranged by size:
926 654 132 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 1194 1771 1833
Size: 1194 577 62
Fragments arranged by size:
1194 577 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 899 1833
Size: 899 934
BclI T'GATC_A
Cuts at: 0 434 1833
Size: 434 1399
BglI GCCn_nnn'nGGC
Cuts at: 0 921 1139 1833
Size: 921 218 694
Fragments arranged by size:
921 694 218
BglII A'GATC_T
Cuts at: 0 278 839 851 1833
Size: 278 561 12 982
Fragments arranged by size:
982 561 278 12
Bme1580I G_kGCm'C
Cuts at: 0 670 1833
Size: 670 1163
BmgBI CAC'GTC
Cuts at: 0 1613 1833
Size: 1613 220
BmtI G_CTAG'C
Cuts at: 0 533 1833
Size: 533 1300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1461 1833
Size: 1461 372
BsaI GGTCTCn'nnnn_
Cuts at: 0 1104 1833
Size: 1104 729
BsaAI yAC'GTr
Cuts at: 0 1311 1833
Size: 1311 522
BsaHI Gr'CG_yC
Cuts at: 0 1778 1833
Size: 1778 55
BsaWI w'CCGG_w
Cuts at: 0 536 1160 1833
Size: 536 624 673
Fragments arranged by size:
673 624 536
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1333 1833
Size: 848 485 500
Fragments arranged by size:
848 500 485
BsiHKAI G_wGCw'C
Cuts at: 0 670 735 1058 1712 1833
Size: 670 65 323 654 121
Fragments arranged by size:
670 654 323 121 65
BsmI GAATG_Cn'
Cuts at: 0 1361 1833
Size: 1361 472
Bsp1286I G_dGCh'C
Cuts at: 0 670 735 926 1058 1712 1833
Size: 670 65 191 132 654 121
Fragments arranged by size:
670 654 191 132 121 65
BspHI T'CATG_A
Cuts at: 0 1058 1833
Size: 1058 775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 1187 1833
Size: 289 898 646
Fragments arranged by size:
898 646 289
BsrDI GCAATG_nn'
Cuts at: 0 45 167 881 1833
Size: 45 122 714 952
Fragments arranged by size:
952 714 122 45
BssSI C'ACGA_G
Cuts at: 0 898 1833
Size: 898 935
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 1833
Size: 324 1509
BstEII G'GTnAC_C
Cuts at: 0 180 1398 1833
Size: 180 1218 435
Fragments arranged by size:
1218 435 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 656 1455 1833
Size: 240 416 799 378
Fragments arranged by size:
799 416 378 240
BstYI r'GATC_y
Cuts at: 0 149 278 333 594 831 839 851 1112
Size: 149 129 55 261 237 8 12 261
Cuts at: 1112 1833
Size: 721
Fragments arranged by size:
721 261 261 237 149 129 55 12
8
BstZ17I GTA'TAC
Cuts at: 0 378 1833
Size: 378 1455
Bsu36I CC'TnA_GG
Cuts at: 0 867 1833
Size: 867 966
BtgI C'CryG_G
Cuts at: 0 479 616 927 1010 1394 1493 1833
Size: 479 137 311 83 384 99 340
Fragments arranged by size:
479 384 340 311 137 99 83
BtsI GCAGTG_nn'
Cuts at: 0 368 1833
Size: 368 1465
DrdI GACnn_nn'nnGTC
Cuts at: 0 1495 1833
Size: 1495 338
EaeI y'GGCC_r
Cuts at: 0 10 344 362 619 1140 1715 1833
Size: 10 334 18 257 521 575 118
Fragments arranged by size:
575 521 334 257 118 18 10
EarI CTCTTCn'nnn_
Cuts at: 0 772 811 1153 1219 1351 1700 1833
Size: 772 39 342 66 132 349 133
Fragments arranged by size:
772 349 342 133 132 66 39
EcoICRI GAG'CTC
Cuts at: 0 1056 1710 1833
Size: 1056 654 123
Fragments arranged by size:
1056 654 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 772 1833
Size: 523 249 1061
Fragments arranged by size:
1061 523 249
EcoO109I rG'GnC_Cy
Cuts at: 0 627 1254 1833
Size: 627 627 579
Fragments arranged by size:
627 627 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 534 1263 1833
Size: 534 729 570
Fragments arranged by size:
729 570 534
HaeII r_GCGC'y
Cuts at: 0 213 454 1344 1833
Size: 213 241 890 489
Fragments arranged by size:
890 489 241 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 1833
Size: 594 32 1207
Fragments arranged by size:
1207 594 32
HincII GTy'rAC
Cuts at: 0 1782 1833
Size: 1782 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 1833
Size: 24 1809
MscI TGG'CCA
Cuts at: 0 12 346 364 1833
Size: 12 334 18 1469
Fragments arranged by size:
1469 334 18 12
MslI CAynn'nnrTG
Cuts at: 0 187 460 775 1063 1628 1833
Size: 187 273 315 288 565 205
Fragments arranged by size:
565 315 288 273 205 187
MspA1I CmG'CkG
Cuts at: 0 481 554 600 690 1628 1833
Size: 481 73 46 90 938 205
Fragments arranged by size:
938 481 205 90 73 46
NcoI C'CATG_G
Cuts at: 0 1010 1833
Size: 1010 823
NheI G'CTAG_C
Cuts at: 0 529 1833
Size: 529 1304
NspI r_CATG'y
Cuts at: 0 294 1833
Size: 294 1539
PasI CC'CwG_GG
Cuts at: 0 784 1833
Size: 784 1049
PciI A'CATG_T
Cuts at: 0 290 1833
Size: 290 1543
PflMI CCAn_nnn'nTGG
Cuts at: 0 1109 1673 1833
Size: 1109 564 160
Fragments arranged by size:
1109 564 160
PfoI T'CCnGG_A
Cuts at: 0 1295 1772 1833
Size: 1295 477 61
Fragments arranged by size:
1295 477 61
PpuMI rG'GwC_Cy
Cuts at: 0 627 1833
Size: 627 1206
PstI C_TGCA'G
Cuts at: 0 428 859 1184 1833
Size: 428 431 325 649
Fragments arranged by size:
649 431 428 325
PvuII CAG'CTG
Cuts at: 0 554 1628 1833
Size: 554 1074 205
Fragments arranged by size:
1074 554 205
SacI G_AGCT'C
Cuts at: 0 1058 1712 1833
Size: 1058 654 121
Fragments arranged by size:
1058 654 121
SacII CC_GC'GG
Cuts at: 0 482 1833
Size: 482 1351
SapI GCTCTTCn'nnn_
Cuts at: 0 1153 1351 1700 1833
Size: 1153 198 349 133
Fragments arranged by size:
1153 349 198 133
ScaI AGT'ACT
Cuts at: 0 514 1833
Size: 514 1319
SexAI A'CCwGG_T
Cuts at: 0 1471 1833
Size: 1471 362
SfcI C'TryA_G
Cuts at: 0 424 468 855 1180 1833
Size: 424 44 387 325 653
Fragments arranged by size:
653 424 387 325 44
SmaI CCC'GGG
Cuts at: 0 568 1833
Size: 568 1265
StyI C'CwwG_G
Cuts at: 0 1010 1448 1757 1833
Size: 1010 438 309 76
Fragments arranged by size:
1010 438 309 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7 1833
Size: 7 1826
TatI w'GTAC_w
Cuts at: 0 246 512 1833
Size: 246 266 1321
Fragments arranged by size:
1321 266 246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 1008 1833
Size: 900 108 825
Fragments arranged by size:
900 825 108
XmaI C'CCGG_G
Cuts at: 0 566 1833
Size: 566 1267
XmnI GAAnn'nnTTC
Cuts at: 0 211 514 1833
Size: 211 303 1319
Fragments arranged by size:
1319 303 211
ZraI GAC'GTC
Cuts at: 0 1779 1833
Size: 1779 54
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlwNI
ApaLI AvaI BamHI BanI BanII BbsI BciVI BclI
BglI BglII Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaHI BsaWI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NcoI NheI NspI PasI PciI PflMI PfoI PpuMI
PstI PvuII SacI SacII SapI ScaI SexAI SfcI
SmaI StyI TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AlfI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BfrBI BlpI BmrI BpmI Bpu10I BpuEI BsaBI
BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BstBI BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI