Restriction map for
pFN21AE6491
(Linear) (Six-base) MAPSORT of: KIEE6491.seq Check: 2034 from: 1 to: 1800
KIEE6491.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 772 832 1146 1800
Size: 772 60 314 654
Fragments arranged by size:
772 654 314 60
AccI GT'mk_AC
Cuts at: 0 674 1800
Size: 674 1126
AclI AA'CG_TT
Cuts at: 0 344 1800
Size: 344 1456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1777 1800
Size: 1777 23
AlwNI CAG_nnn'CTG
Cuts at: 0 824 893 1061 1320 1640 1800
Size: 824 69 168 259 320 160
Fragments arranged by size:
824 320 259 168 160 69
AvaI C'yCGr_G
Cuts at: 0 58 176 1109 1800
Size: 58 118 933 691
Fragments arranged by size:
933 691 118 58
BanI G'GyrC_C
Cuts at: 0 3 834 1104 1134 1689 1800
Size: 3 831 270 30 555 111
Fragments arranged by size:
831 555 270 111 30 3
BanII G_rGCy'C
Cuts at: 0 606 857 865 938 1082 1648 1800
Size: 606 251 8 73 144 566 152
Fragments arranged by size:
606 566 251 152 144 73 8
BbeI G_GCGC'C
Cuts at: 0 7 838 1800
Size: 7 831 962
Fragments arranged by size:
962 831 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 453 1800
Size: 453 1347
BglI GCCn_nnn'nGGC
Cuts at: 0 997 1132 1800
Size: 997 135 668
Fragments arranged by size:
997 668 135
BglII A'GATC_T
Cuts at: 0 398 1235 1800
Size: 398 837 565
Fragments arranged by size:
837 565 398
Bme1580I G_kGCm'C
Cuts at: 0 1042 1800
Size: 1042 758
BmgBI CAC'GTC
Cuts at: 0 105 1800
Size: 105 1695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1207 1456 1662 1800
Size: 139 1068 249 206 138
Fragments arranged by size:
1068 249 206 139 138
Bpu10I CC'TnA_GC
Cuts at: 0 851 932 1800
Size: 851 81 868
Fragments arranged by size:
868 851 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 178 343 694 1534 1800
Size: 94 84 165 351 840 266
Fragments arranged by size:
840 351 266 165 94 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 608 932 1800
Size: 608 324 868
Fragments arranged by size:
868 608 324
BsaAI yAC'GTr
Cuts at: 0 1787 1800
Size: 1787 13
BsaHI Gr'CG_yC
Cuts at: 0 4 443 835 1800
Size: 4 439 392 965
Fragments arranged by size:
965 439 392 4
BsaWI w'CCGG_w
Cuts at: 0 161 1192 1800
Size: 161 1031 608
Fragments arranged by size:
1031 608 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 289 319 1204 1222 1234 1252
Size: 256 30 3 30 885 18 12 18
Cuts at: 1252 1800
Size: 548
Fragments arranged by size:
885 548 256 30 30 18 18 12
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 1525 1800
Size: 409 1116 275
Fragments arranged by size:
1116 409 275
BseYI C'CCAG_C
Cuts at: 0 7 37 436 886 909 1800
Size: 7 30 399 450 23 891
Fragments arranged by size:
891 450 399 30 23 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1723 1800
Size: 637 1086 77
Fragments arranged by size:
1086 637 77
BsiEI CG_ry'CG
Cuts at: 0 261 1707 1800
Size: 261 1446 93
Fragments arranged by size:
1446 261 93
BsiHKAI G_wGCw'C
Cuts at: 0 104 1648 1753 1800
Size: 104 1544 105 47
Fragments arranged by size:
1544 105 104 47
BsmI GAATG_Cn'
Cuts at: 0 627 1800
Size: 627 1173
BsmBI CGTCTCn'nnnn_
Cuts at: 0 771 1093 1800
Size: 771 322 707
Fragments arranged by size:
771 707 322
Bsp1286I G_dGCh'C
Cuts at: 0 104 606 857 865 938 1042 1082 1648
Size: 104 502 251 8 73 104 40 566
Cuts at: 1648 1753 1800
Size: 105 47
Fragments arranged by size:
566 502 251 105 104 104 73 47
40 8
BspEI T'CCGG_A
Cuts at: 0 161 1192 1800
Size: 161 1031 608
Fragments arranged by size:
1031 608 161
BspHI T'CATG_A
Cuts at: 0 497 1676 1800
Size: 497 1179 124
Fragments arranged by size:
1179 497 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 772 832 1146 1286 1800
Size: 363 409 60 314 140 514
Fragments arranged by size:
514 409 363 314 140 60
BsrBI CCG'CTC
Cuts at: 0 24 44 123 479 1296 1800
Size: 24 20 79 356 817 504
Fragments arranged by size:
817 504 356 79 24 20
BsrDI GCAATG_nn'
Cuts at: 0 947 1800
Size: 947 853
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1061 1661 1800
Size: 1061 600 139
Fragments arranged by size:
1061 600 139
BstBI TT'CG_AA
Cuts at: 0 1349 1800
Size: 1349 451
BstYI r'GATC_y
Cuts at: 0 398 1235 1577 1800
Size: 398 837 342 223
Fragments arranged by size:
837 398 342 223
Bsu36I CC'TnA_GG
Cuts at: 0 899 1016 1800
Size: 899 117 784
Fragments arranged by size:
899 784 117
BtgI C'CryG_G
Cuts at: 0 551 1800
Size: 551 1249
BtsI GCAGTG_nn'
Cuts at: 0 294 1641 1800
Size: 294 1347 159
Fragments arranged by size:
1347 294 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 541 1800
Size: 541 1259
EaeI y'GGCC_r
Cuts at: 0 258 482 554 1269 1704 1800
Size: 258 224 72 715 435 96
Fragments arranged by size:
715 435 258 224 96 72
EagI C'GGCC_G
Cuts at: 0 258 1704 1800
Size: 258 1446 96
Fragments arranged by size:
1446 258 96
EarI CTCTTCn'nnn_
Cuts at: 0 163 1800
Size: 163 1637
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 1363 1800
Size: 973 390 437
Fragments arranged by size:
973 437 390
EcoICRI GAG'CTC
Cuts at: 0 1646 1800
Size: 1646 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1207 1456 1662 1777 1800
Size: 139 1068 249 206 115 23
Fragments arranged by size:
1068 249 206 139 115 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1609 1800
Size: 1609 191
EcoO109I rG'GnC_Cy
Cuts at: 0 1011 1045 1461 1563 1800
Size: 1011 34 416 102 237
Fragments arranged by size:
1011 416 237 102 34
FspI TGC'GCA
Cuts at: 0 1070 1800
Size: 1070 730
FspAI rTGC'GCAy
Cuts at: 0 1070 1800
Size: 1070 730
HaeII r_GCGC'y
Cuts at: 0 7 838 1738 1800
Size: 7 831 900 62
Fragments arranged by size:
900 831 62 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 1222 1254 1800
Size: 289 32 901 32 546
Fragments arranged by size:
901 546 289 32 32
HincII GTy'rAC
Cuts at: 0 1161 1716 1800
Size: 1161 555 84
Fragments arranged by size:
1161 555 84
KasI G'GCGC_C
Cuts at: 0 3 834 1800
Size: 3 831 966
Fragments arranged by size:
966 831 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1548 1800
Size: 1202 346 252
Fragments arranged by size:
1202 346 252
MslI CAynn'nnrTG
Cuts at: 0 502 508 1675 1800
Size: 502 6 1167 125
Fragments arranged by size:
1167 502 125 6
MspA1I CmG'CkG
Cuts at: 0 32 117 137 575 1061 1631 1703 1800
Size: 32 85 20 438 486 570 72 97
Fragments arranged by size:
570 486 438 97 85 72 32 20
NarI GG'CG_CC
Cuts at: 0 4 835 1800
Size: 4 831 965
Fragments arranged by size:
965 831 4
NotI GC'GGCC_GC
Cuts at: 0 258 1704 1800
Size: 258 1446 96
Fragments arranged by size:
1446 258 96
PfoI T'CCnGG_A
Cuts at: 0 386 1800
Size: 386 1414
PpuMI rG'GwC_Cy
Cuts at: 0 1011 1045 1461 1563 1800
Size: 1011 34 416 102 237
Fragments arranged by size:
1011 416 237 102 34
PshAI GACnn'nnGTC
Cuts at: 0 1303 1612 1800
Size: 1303 309 188
Fragments arranged by size:
1303 309 188
PvuII CAG'CTG
Cuts at: 0 117 1800
Size: 117 1683
SacI G_AGCT'C
Cuts at: 0 1648 1800
Size: 1648 152
SanDI GG'GwC_CC
Cuts at: 0 1011 1800
Size: 1011 789
SexAI A'CCwGG_T
Cuts at: 0 665 719 1457 1800
Size: 665 54 738 343
Fragments arranged by size:
738 665 343 54
SfcI C'TryA_G
Cuts at: 0 402 1800
Size: 402 1398
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 997 1800
Size: 997 803
SfoI GGC'GCC
Cuts at: 0 5 836 1800
Size: 5 831 964
Fragments arranged by size:
964 831 5
SmaI CCC'GGG
Cuts at: 0 60 178 1111 1800
Size: 60 118 933 689
Fragments arranged by size:
933 689 118 60
SmlI C'TyrA_G
Cuts at: 0 109 193 358 709 1549 1800
Size: 109 84 165 351 840 251
Fragments arranged by size:
840 351 251 165 109 84
StyI C'CwwG_G
Cuts at: 0 449 959 1001 1800
Size: 449 510 42 799
Fragments arranged by size:
799 510 449 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 623 1800
Size: 623 1177
TatI w'GTAC_w
Cuts at: 0 326 1175 1800
Size: 326 849 625
Fragments arranged by size:
849 625 326
Tth111I GACn'n_nGTC
Cuts at: 0 1426 1672 1800
Size: 1426 246 128
Fragments arranged by size:
1426 246 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 747 966 1551 1800
Size: 666 81 219 585 249
Fragments arranged by size:
666 585 249 219 81
XmaI C'CCGG_G
Cuts at: 0 58 176 1109 1800
Size: 58 118 933 691
Fragments arranged by size:
933 691 118 58
Enzymes that do cut:
AarI AccI AclI AcuI AlwNI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BstAPI BstBI BstYI Bsu36I BtgI
BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII KasI
MmeI MslI MspA1I NarI NotI PfoI PpuMI PshAI
PvuII SacI SanDI SexAI SfcI SfiI SfoI SmaI
SmlI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI BplI BsaBI BsiWI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII EcoRI EcoRV FalI FseI HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XhoI XmnI ZraI