Restriction map for
pFN21AE6488
(Linear) (Six-base) MAPSORT of: KIEE6488.seq Check: 8638 from: 1 to: 1677
KIEE6488.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 182 1114 1677
Size: 182 932 563
Fragments arranged by size:
932 563 182
AclI AA'CG_TT
Cuts at: 0 448 1677
Size: 448 1229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1462 1666 1677
Size: 139 1323 204 11
Fragments arranged by size:
1323 204 139 11
AflIII A'CryG_T
Cuts at: 0 473 1677
Size: 473 1204
AleI CACnn'nnGTG
Cuts at: 0 55 757 1677
Size: 55 702 920
Fragments arranged by size:
920 702 55
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 1677
Size: 73 1604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 1677
Size: 475 32 1170
Fragments arranged by size:
1170 475 32
AlwNI CAG_nnn'CTG
Cuts at: 0 335 1677
Size: 335 1342
ApaI G_GGCC'C
Cuts at: 0 1642 1677
Size: 1642 35
AvaI C'yCGr_G
Cuts at: 0 374 1677
Size: 374 1303
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 7 40 1677
Size: 7 33 1637
Fragments arranged by size:
1637 33 7
BamHI G'GATC_C
Cuts at: 0 284 1677
Size: 284 1393
BanI G'GyrC_C
Cuts at: 0 847 1677
Size: 847 830
BanII G_rGCy'C
Cuts at: 0 103 1526 1642 1677
Size: 103 1423 116 35
Fragments arranged by size:
1423 116 103 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1065 1677
Size: 1065 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1161 1195 1677
Size: 1161 34 482
Fragments arranged by size:
1161 482 34
BclI T'GATC_A
Cuts at: 0 110 1677
Size: 110 1567
BglI GCCn_nnn'nGGC
Cuts at: 0 52 577 1677
Size: 52 525 1100
Fragments arranged by size:
1100 525 52
BlpI GC'TnA_GC
Cuts at: 0 259 781 1654 1677
Size: 259 522 873 23
Fragments arranged by size:
873 522 259 23
Bme1580I G_kGCm'C
Cuts at: 0 151 850 1174 1605 1642 1677
Size: 151 699 324 431 37 35
Fragments arranged by size:
699 431 324 151 37 35
BmgBI CAC'GTC
Cuts at: 0 381 1677
Size: 381 1296
BmrI ACTGGGnnnn_n'
Cuts at: 0 289 1677
Size: 289 1388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 823 1006 1315 1594 1677
Size: 82 741 183 309 279 83
Fragments arranged by size:
741 309 279 183 83 82
BsaAI yAC'GTr
Cuts at: 0 146 1677
Size: 146 1531
BsaHI Gr'CG_yC
Cuts at: 0 1157 1677
Size: 1157 520
BsaWI w'CCGG_w
Cuts at: 0 26 1677
Size: 26 1651
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1677
Size: 475 30 1172
Fragments arranged by size:
1172 475 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 913 1056 1677
Size: 913 143 621
Fragments arranged by size:
913 621 143
BseYI C'CCAG_C
Cuts at: 0 95 150 796 941 1677
Size: 95 55 646 145 736
Fragments arranged by size:
736 646 145 95 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 336 621 1336 1677
Size: 33 303 285 715 341
Fragments arranged by size:
715 341 303 285 33
BsiHKAI G_wGCw'C
Cuts at: 0 260 1677
Size: 260 1417
Bsp1286I G_dGCh'C
Cuts at: 0 103 151 260 850 1174 1526 1605 1642
Size: 103 48 109 590 324 352 79 37
Cuts at: 1642 1677
Size: 35
Fragments arranged by size:
590 352 324 109 103 79 48 37
35
BspHI T'CATG_A
Cuts at: 0 509 1677
Size: 509 1168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 494 1114 1289 1677
Size: 182 312 620 175 388
Fragments arranged by size:
620 388 312 182 175
BsrFI r'CCGG_y
Cuts at: 0 571 1677
Size: 571 1106
BstAPI GCAn_nnn'nTGC
Cuts at: 0 335 1677
Size: 335 1342
BstEII G'GTnAC_C
Cuts at: 0 739 1668 1677
Size: 739 929 9
Fragments arranged by size:
929 739 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 1635 1677
Size: 501 1134 42
Fragments arranged by size:
1134 501 42
BstYI r'GATC_y
Cuts at: 0 284 1235 1677
Size: 284 951 442
Fragments arranged by size:
951 442 284
Bsu36I CC'TnA_GG
Cuts at: 0 674 1677
Size: 674 1003
BtgI C'CryG_G
Cuts at: 0 767 929 1677
Size: 767 162 748
Fragments arranged by size:
767 748 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1513 1677
Size: 1513 164
BtsI GCAGTG_nn'
Cuts at: 0 336 341 1412 1677
Size: 336 5 1071 265
Fragments arranged by size:
1071 336 265 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 690 725 1677
Size: 690 35 952
Fragments arranged by size:
952 690 35
DraIII CAC_nnn'GTG
Cuts at: 0 732 1677
Size: 732 945
EaeI y'GGCC_r
Cuts at: 0 196 1005 1677
Size: 196 809 672
Fragments arranged by size:
809 672 196
EarI CTCTTCn'nnn_
Cuts at: 0 76 534 751 1594 1677
Size: 76 458 217 843 83
Fragments arranged by size:
843 458 217 83 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1152 1677
Size: 1152 525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 139 823 1006 1315 1462 1594 1666
Size: 82 57 684 183 309 147 132 72
Cuts at: 1666 1677
Size: 11
Fragments arranged by size:
684 309 183 147 132 82 72 57
11
EcoO109I rG'GnC_Cy
Cuts at: 0 792 1507 1677
Size: 792 715 170
Fragments arranged by size:
792 715 170
HaeII r_GCGC'y
Cuts at: 0 36 266 415 1677
Size: 36 230 149 1262
Fragments arranged by size:
1262 230 149 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1442 1677
Size: 1442 235
MscI TGG'CCA
Cuts at: 0 198 1007 1677
Size: 198 809 670
Fragments arranged by size:
809 670 198
MslI CAynn'nnrTG
Cuts at: 0 55 358 499 757 934 1498 1677
Size: 55 303 141 258 177 564 179
Fragments arranged by size:
564 303 258 179 177 141 55
MspA1I CmG'CkG
Cuts at: 0 154 945 1089 1677
Size: 154 791 144 588
Fragments arranged by size:
791 588 154 144
NcoI C'CATG_G
Cuts at: 0 767 929 1677
Size: 767 162 748
Fragments arranged by size:
767 748 162
NspI r_CATG'y
Cuts at: 0 477 1677
Size: 477 1200
PciI A'CATG_T
Cuts at: 0 473 1677
Size: 473 1204
PmlI CAC'GTG
Cuts at: 0 146 1677
Size: 146 1531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 1677
Size: 475 32 1170
Fragments arranged by size:
1170 475 32
PpuMI rG'GwC_Cy
Cuts at: 0 1507 1677
Size: 1507 170
PshAI GACnn'nnGTC
Cuts at: 0 1345 1677
Size: 1345 332
PspOMI G'GGCC_C
Cuts at: 0 1638 1677
Size: 1638 39
PstI C_TGCA'G
Cuts at: 0 1407 1448 1677
Size: 1407 41 229
Fragments arranged by size:
1407 229 41
PvuII CAG'CTG
Cuts at: 0 154 945 1089 1677
Size: 154 791 144 588
Fragments arranged by size:
791 588 154 144
SanDI GG'GwC_CC
Cuts at: 0 1507 1677
Size: 1507 170
SexAI A'CCwGG_T
Cuts at: 0 601 1677
Size: 601 1076
SfcI C'TryA_G
Cuts at: 0 1403 1444 1677
Size: 1403 41 233
Fragments arranged by size:
1403 233 41
SmaI CCC'GGG
Cuts at: 0 376 1677
Size: 376 1301
StuI AGG'CCT
Cuts at: 0 1442 1677
Size: 1442 235
StyI C'CwwG_G
Cuts at: 0 767 929 1677
Size: 767 162 748
Fragments arranged by size:
767 748 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 769 1521 1677
Size: 190 579 752 156
Fragments arranged by size:
752 579 190 156
TatI w'GTAC_w
Cuts at: 0 84 1677
Size: 84 1593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 1677
Size: 269 32 1376
Fragments arranged by size:
1376 269 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 1384 1677
Size: 276 1108 293
Fragments arranged by size:
1108 293 276
XmaI C'CCGG_G
Cuts at: 0 374 1677
Size: 374 1303
XmnI GAAnn'nnTTC
Cuts at: 0 70 662 860 1677
Size: 70 592 198 817
Fragments arranged by size:
817 592 198 70
Enzymes that do cut:
AarI AclI AcuI AflIII AleI AlfI AloI AlwNI
ApaI AvaI BaeI BamHI BanI BanII BbsI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI
Eco57MI EcoO109I HaeII MmeI MscI MslI MspA1I NcoI
NspI PciI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SanDI SexAI SfcI SmaI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI ApaLI
ApoI AscI AseI AsiSI AvrII BbeI BbvCI BciVI
BfrBI BglII BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstZ17I ClaI DraI DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI ZraI