Restriction map for
pFN21AE6486
(Linear) (Six-base) MAPSORT of: KIEE6486.seq Check: 8394 from: 1 to: 1752
KIEE6486.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 91 152 1752
Size: 91 61 1600
Fragments arranged by size:
1600 91 61
Acc65I G'GTAC_C
Cuts at: 0 236 1752
Size: 236 1516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 418 447 591 1190 1752
Size: 110 308 29 144 599 562
Fragments arranged by size:
599 562 308 144 110 29
AleI CACnn'nnGTG
Cuts at: 0 550 1315 1549 1752
Size: 550 765 234 203
Fragments arranged by size:
765 550 234 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1384 1752
Size: 1384 368
AlwNI CAG_nnn'CTG
Cuts at: 0 73 912 1752
Size: 73 839 840
Fragments arranged by size:
840 839 73
ApaI G_GGCC'C
Cuts at: 0 924 1752
Size: 924 828
ApaLI G'TGCA_C
Cuts at: 0 449 1752
Size: 449 1303
ApoI r'AATT_y
Cuts at: 0 1723 1752
Size: 1723 29
BanI G'GyrC_C
Cuts at: 0 236 1594 1752
Size: 236 1358 158
Fragments arranged by size:
1358 236 158
BanII G_rGCy'C
Cuts at: 0 88 102 690 924 1379 1607 1752
Size: 88 14 588 234 455 228 145
Fragments arranged by size:
588 455 234 228 145 88 14
BbeI G_GCGC'C
Cuts at: 0 1598 1752
Size: 1598 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 1341 1608 1752
Size: 1341 267 144
Fragments arranged by size:
1341 267 144
BglI GCCn_nnn'nGGC
Cuts at: 0 475 505 514 1245 1752
Size: 475 30 9 731 507
Fragments arranged by size:
731 507 475 30 9
BglII A'GATC_T
Cuts at: 0 1529 1752
Size: 1529 223
BlpI GC'TnA_GC
Cuts at: 0 1357 1606 1654 1752
Size: 1357 249 48 98
Fragments arranged by size:
1357 249 98 48
Bme1580I G_kGCm'C
Cuts at: 0 33 453 471 519 924 1752
Size: 33 420 18 48 405 828
Fragments arranged by size:
828 420 405 48 33 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 108 1752
Size: 108 1644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 788 825 1752
Size: 788 37 927
Fragments arranged by size:
927 788 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 782 1510 1579 1752
Size: 579 203 728 69 173
Fragments arranged by size:
728 579 203 173 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1752
Size: 964 788
BsaI GGTCTCn'nnnn_
Cuts at: 0 889 1632 1752
Size: 889 743 120
Fragments arranged by size:
889 743 120
BsaHI Gr'CG_yC
Cuts at: 0 422 1595 1752
Size: 422 1173 157
Fragments arranged by size:
1173 422 157
BsaWI w'CCGG_w
Cuts at: 0 203 1752
Size: 203 1549
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 1752
Size: 55 30 1667
Fragments arranged by size:
1667 55 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 835 1006 1672 1705 1752
Size: 274 561 171 666 33 47
Fragments arranged by size:
666 561 274 171 47 33
BseYI C'CCAG_C
Cuts at: 0 26 277 1371 1512 1752
Size: 26 251 1094 141 240
Fragments arranged by size:
1094 251 240 141 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 1752
Size: 723 1029
BsiEI CG_ry'CG
Cuts at: 0 877 1307 1752
Size: 877 430 445
Fragments arranged by size:
877 445 430
BsiHKAI G_wGCw'C
Cuts at: 0 102 453 1752
Size: 102 351 1299
Fragments arranged by size:
1299 351 102
Bsp1286I G_dGCh'C
Cuts at: 0 33 88 102 453 471 519 690 924
Size: 33 55 14 351 18 48 171 234
Cuts at: 924 1379 1607 1752
Size: 455 228 145
Fragments arranged by size:
455 351 234 228 171 145 55 48
33 18 14
BspEI T'CCGG_A
Cuts at: 0 203 1752
Size: 203 1549
BsrDI GCAATG_nn'
Cuts at: 0 1636 1752
Size: 1636 116
BsrFI r'CCGG_y
Cuts at: 0 4 499 872 1752
Size: 4 495 373 880
Fragments arranged by size:
880 495 373 4
BssSI C'ACGA_G
Cuts at: 0 1498 1752
Size: 1498 254
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 1752
Size: 263 1489
BstEII G'GTnAC_C
Cuts at: 0 540 672 694 1752
Size: 540 132 22 1058
Fragments arranged by size:
1058 540 132 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 229 552 1317 1752
Size: 229 323 765 435
Fragments arranged by size:
765 435 323 229
BstYI r'GATC_y
Cuts at: 0 746 769 803 908 1529 1752
Size: 746 23 34 105 621 223
Fragments arranged by size:
746 621 223 105 34 23
BtgI C'CryG_G
Cuts at: 0 146 663 899 1450 1752
Size: 146 517 236 551 302
Fragments arranged by size:
551 517 302 236 146
BtsI GCAGTG_nn'
Cuts at: 0 1648 1752
Size: 1648 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 150 185 1752
Size: 150 35 1567
Fragments arranged by size:
1567 150 35
DraIII CAC_nnn'GTG
Cuts at: 0 37 414 1752
Size: 37 377 1338
Fragments arranged by size:
1338 377 37
DrdI GACnn_nn'nnGTC
Cuts at: 0 1122 1752
Size: 1122 630
EaeI y'GGCC_r
Cuts at: 0 2 380 554 874 1304 1752
Size: 2 378 174 320 430 448
Fragments arranged by size:
448 430 378 320 174 2
EagI C'GGCC_G
Cuts at: 0 874 1304 1752
Size: 874 430 448
Fragments arranged by size:
874 448 430
EarI CTCTTCn'nnn_
Cuts at: 0 664 1690 1752
Size: 664 1026 62
Fragments arranged by size:
1026 664 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 1455 1752
Size: 76 1379 297
Fragments arranged by size:
1379 297 76
EcoICRI GAG'CTC
Cuts at: 0 100 1752
Size: 100 1652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 418 447 579 591 782 1190 1510
Size: 110 308 29 132 12 191 408 320
Cuts at: 1510 1579 1752
Size: 69 173
Fragments arranged by size:
408 320 308 191 173 132 110 69
29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 606 1752
Size: 606 1146
EcoO109I rG'GnC_Cy
Cuts at: 0 599 921 1130 1584 1752
Size: 599 322 209 454 168
Fragments arranged by size:
599 454 322 209 168
HaeII r_GCGC'y
Cuts at: 0 530 1289 1598 1752
Size: 530 759 309 154
Fragments arranged by size:
759 530 309 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 817 849 888 920 1402 1434
Size: 55 32 730 32 39 32 482 32
Cuts at: 1434 1752
Size: 318
Fragments arranged by size:
730 482 318 55 39 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1594 1752
Size: 1594 158
KpnI G_GTAC'C
Cuts at: 0 240 1752
Size: 240 1512
MslI CAynn'nnrTG
Cuts at: 0 227 550 1315 1498 1549 1641 1752
Size: 227 323 765 183 51 92 111
Fragments arranged by size:
765 323 227 183 111 92 51
MspA1I CmG'CkG
Cuts at: 0 191 879 1752
Size: 191 688 873
Fragments arranged by size:
873 688 191
NaeI GCC'GGC
Cuts at: 0 6 501 1752
Size: 6 495 1251
Fragments arranged by size:
1251 495 6
NarI GG'CG_CC
Cuts at: 0 1595 1752
Size: 1595 157
NgoMIV G'CCGG_C
Cuts at: 0 4 499 1752
Size: 4 495 1253
Fragments arranged by size:
1253 495 4
NotI GC'GGCC_GC
Cuts at: 0 1304 1752
Size: 1304 448
PasI CC'CwG_GG
Cuts at: 0 1338 1599 1752
Size: 1338 261 153
Fragments arranged by size:
1338 261 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 414 1752
Size: 414 1338
PpuMI rG'GwC_Cy
Cuts at: 0 1130 1584 1752
Size: 1130 454 168
Fragments arranged by size:
1130 454 168
PspOMI G'GGCC_C
Cuts at: 0 920 1752
Size: 920 832
PstI C_TGCA'G
Cuts at: 0 1124 1752
Size: 1124 628
SacI G_AGCT'C
Cuts at: 0 102 1752
Size: 102 1650
SapI GCTCTTCn'nnn_
Cuts at: 0 664 1752
Size: 664 1088
SfcI C'TryA_G
Cuts at: 0 92 1120 1752
Size: 92 1028 632
Fragments arranged by size:
1028 632 92
SfoI GGC'GCC
Cuts at: 0 1596 1752
Size: 1596 156
SmlI C'TyrA_G
Cuts at: 0 979 1752
Size: 979 773
StyI C'CwwG_G
Cuts at: 0 510 1199 1752
Size: 510 689 553
Fragments arranged by size:
689 553 510
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1330 1492 1752
Size: 1330 162 260
Fragments arranged by size:
1330 260 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1122 1154 1562 1594 1752
Size: 1122 32 408 32 158
Fragments arranged by size:
1122 408 158 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 69 1752
Size: 69 1683
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApaI ApaLI
ApoI BanI BanII BbeI BbsI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI
PpuMI PspOMI PstI SacI SapI SfcI SfoI SmlI
StyI TaqII TstI Tth111I
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI Bpu10I
BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI DraI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XcmI XhoI XmaI XmnI ZraI