Restriction map for
pFN21AE6484
(Linear) (Six-base) MAPSORT of: KIEE6484.seq Check: 34 from: 1 to: 1863
KIEE6484.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 659 1315 1863
Size: 659 656 548
Fragments arranged by size:
659 656 548
Acc65I G'GTAC_C
Cuts at: 0 1197 1863
Size: 1197 666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1309 1863
Size: 431 878 554
Fragments arranged by size:
878 554 431
AflIII A'CryG_T
Cuts at: 0 975 1863
Size: 975 888
AleI CACnn'nnGTG
Cuts at: 0 1807 1863
Size: 1807 56
ApaLI G'TGCA_C
Cuts at: 0 110 1863
Size: 110 1753
ApoI r'AATT_y
Cuts at: 0 82 1498 1863
Size: 82 1416 365
Fragments arranged by size:
1416 365 82
AvaI C'yCGr_G
Cuts at: 0 446 1863
Size: 446 1417
BanI G'GyrC_C
Cuts at: 0 195 821 1197 1736 1863
Size: 195 626 376 539 127
Fragments arranged by size:
626 539 376 195 127
BanII G_rGCy'C
Cuts at: 0 256 431 1863
Size: 256 175 1432
Fragments arranged by size:
1432 256 175
BbeI G_GCGC'C
Cuts at: 0 825 1863
Size: 825 1038
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 196 1179 1863
Size: 29 167 983 684
Fragments arranged by size:
983 684 167 29
BglII A'GATC_T
Cuts at: 0 96 1432 1863
Size: 96 1336 431
Fragments arranged by size:
1336 431 96
BlpI GC'TnA_GC
Cuts at: 0 1477 1863
Size: 1477 386
Bme1580I G_kGCm'C
Cuts at: 0 114 1863
Size: 114 1749
BmgBI CAC'GTC
Cuts at: 0 202 1863
Size: 202 1661
BmrI ACTGGGnnnn_n'
Cuts at: 0 609 1863
Size: 609 1254
Bpu10I CC'TnA_GC
Cuts at: 0 329 1248 1863
Size: 329 919 615
Fragments arranged by size:
919 615 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1137 1863
Size: 1035 102 726
Fragments arranged by size:
1035 726 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 773 1863
Size: 773 1090
BsaAI yAC'GTr
Cuts at: 0 976 1863
Size: 976 887
BsaBI GATnn'nnATC
Cuts at: 0 101 1863
Size: 101 1762
BsaHI Gr'CG_yC
Cuts at: 0 822 1863
Size: 822 1041
BsaWI w'CCGG_w
Cuts at: 0 1718 1863
Size: 1718 145
BsiEI CG_ry'CG
Cuts at: 0 1719 1863
Size: 1719 144
BsiHKAI G_wGCw'C
Cuts at: 0 114 1863
Size: 114 1749
Bsp1286I G_dGCh'C
Cuts at: 0 114 256 431 1863
Size: 114 142 175 1432
Fragments arranged by size:
1432 175 142 114
BspHI T'CATG_A
Cuts at: 0 77 1118 1863
Size: 77 1041 745
Fragments arranged by size:
1041 745 77
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 699 883 923 1863
Size: 699 184 40 940
Fragments arranged by size:
940 699 184 40
BsrDI GCAATG_nn'
Cuts at: 0 888 1863
Size: 888 975
BstEII G'GTnAC_C
Cuts at: 0 457 1863
Size: 457 1406
BstXI CCAn_nnnn'nTGG
Cuts at: 0 263 1863
Size: 263 1600
BstYI r'GATC_y
Cuts at: 0 96 1432 1615 1863
Size: 96 1336 183 248
Fragments arranged by size:
1336 248 183 96
BstZ17I GTA'TAC
Cuts at: 0 1316 1863
Size: 1316 547
BtgI C'CryG_G
Cuts at: 0 1595 1863
Size: 1595 268
ClaI AT'CG_AT
Cuts at: 0 287 1863
Size: 287 1576
DraI TTT'AAA
Cuts at: 0 1265 1863
Size: 1265 598
DraIII CAC_nnn'GTG
Cuts at: 0 617 1863
Size: 617 1246
DrdI GACnn_nn'nnGTC
Cuts at: 0 1183 1863
Size: 1183 680
EaeI y'GGCC_r
Cuts at: 0 265 1380 1863
Size: 265 1115 483
Fragments arranged by size:
1115 483 265
EarI CTCTTCn'nnn_
Cuts at: 0 240 601 1528 1863
Size: 240 361 927 335
Fragments arranged by size:
927 361 335 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1309 1863
Size: 431 878 554
Fragments arranged by size:
878 554 431
EcoRV GAT'ATC
Cuts at: 0 1678 1863
Size: 1678 185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 943 1863
Size: 943 920
HaeII r_GCGC'y
Cuts at: 0 825 1863
Size: 825 1038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 519 551 770 802 1651 1683 1819 1851
Size: 519 32 219 32 849 32 136 32
Cuts at: 1851 1863
Size: 12
Fragments arranged by size:
849 519 219 136 32 32 32 32
12
HincII GTy'rAC
Cuts at: 0 678 1863
Size: 678 1185
KasI G'GCGC_C
Cuts at: 0 821 1863
Size: 821 1042
KpnI G_GTAC'C
Cuts at: 0 1201 1863
Size: 1201 662
MfeI C'AATT_G
Cuts at: 0 574 1863
Size: 574 1289
MscI TGG'CCA
Cuts at: 0 267 1382 1863
Size: 267 1115 481
Fragments arranged by size:
1115 481 267
MslI CAynn'nnrTG
Cuts at: 0 107 640 1405 1685 1807 1863
Size: 107 533 765 280 122 56
Fragments arranged by size:
765 533 280 122 107 56
MspA1I CmG'CkG
Cuts at: 0 124 1863
Size: 124 1739
NarI GG'CG_CC
Cuts at: 0 822 1863
Size: 822 1041
NcoI C'CATG_G
Cuts at: 0 1595 1863
Size: 1595 268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 1033 1065 1863
Size: 58 32 943 32 798
Fragments arranged by size:
943 798 58 32 32
PvuII CAG'CTG
Cuts at: 0 124 1863
Size: 124 1739
SapI GCTCTTCn'nnn_
Cuts at: 0 1528 1863
Size: 1528 335
SfcI C'TryA_G
Cuts at: 0 538 660 1863
Size: 538 122 1203
Fragments arranged by size:
1203 538 122
SfoI GGC'GCC
Cuts at: 0 823 1863
Size: 823 1040
SmlI C'TyrA_G
Cuts at: 0 1050 1152 1863
Size: 1050 102 711
Fragments arranged by size:
1050 711 102
StyI C'CwwG_G
Cuts at: 0 214 383 746 1595 1863
Size: 214 169 363 849 268
Fragments arranged by size:
849 363 268 214 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 628 1863
Size: 183 445 1235
Fragments arranged by size:
1235 445 183
XbaI T'CTAG_A
Cuts at: 0 1837 1863
Size: 1837 26
XmnI GAAnn'nnTTC
Cuts at: 0 98 1126 1298 1402 1863
Size: 98 1028 172 104 461
Fragments arranged by size:
1028 461 172 104 98
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BglII BlpI Bme1580I BmgBI
BmrI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstEII BstXI
BstYI BstZ17I BtgI ClaI DraI DraIII DrdI EaeI
EarI Eco57MI EcoRV FalI HaeII Hin4I HincII KasI
KpnI MfeI MscI MslI MspA1I NarI NcoI PpiI
PvuII SapI SfcI SfoI SmlI StyI TaqII XbaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglI BmtI
BplI BpmI BsaXI BseRI BseYI BsgI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI Bsu36I BtgZI BtsI CspCI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI FseI FspI FspAI HindIII HpaI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XcmI
XhoI XmaI ZraI