Restriction map for
pFN21AE6481
(Linear) (Six-base) MAPSORT of: KIEE6481.seq Check: 3233 from: 1 to: 1539
KIEE6481.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 225 1539
Size: 225 1314
AatII G_ACGT'C
Cuts at: 0 518 1539
Size: 518 1021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1477 1539
Size: 1477 62
AleI CACnn'nnGTG
Cuts at: 0 110 1539
Size: 110 1429
AlwNI CAG_nnn'CTG
Cuts at: 0 47 1539
Size: 47 1492
ApaI G_GGCC'C
Cuts at: 0 149 1381 1539
Size: 149 1232 158
Fragments arranged by size:
1232 158 149
ApoI r'AATT_y
Cuts at: 0 319 829 1339 1539
Size: 319 510 510 200
Fragments arranged by size:
510 510 319 200
AvaI C'yCGr_G
Cuts at: 0 65 478 1539
Size: 65 413 1061
Fragments arranged by size:
1061 413 65
AvrII C'CTAG_G
Cuts at: 0 54 1539
Size: 54 1485
BamHI G'GATC_C
Cuts at: 0 1018 1539
Size: 1018 521
BanI G'GyrC_C
Cuts at: 0 253 930 938 1539
Size: 253 677 8 601
Fragments arranged by size:
677 601 253 8
BanII G_rGCy'C
Cuts at: 0 35 149 572 1381 1539
Size: 35 114 423 809 158
Fragments arranged by size:
809 423 158 114 35
BbeI G_GCGC'C
Cuts at: 0 942 1539
Size: 942 597
BbsI GAAGACnn'nnnn_
Cuts at: 0 313 1539
Size: 313 1226
BglI GCCn_nnn'nGGC
Cuts at: 0 30 1288 1539
Size: 30 1258 251
Fragments arranged by size:
1258 251 30
BglII A'GATC_T
Cuts at: 0 596 1109 1142 1539
Size: 596 513 33 397
Fragments arranged by size:
596 513 397 33
Bme1580I G_kGCm'C
Cuts at: 0 149 1381 1539
Size: 149 1232 158
Fragments arranged by size:
1232 158 149
BmgBI CAC'GTC
Cuts at: 0 1275 1539
Size: 1275 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 1539
Size: 174 1365
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1410 1539
Size: 1410 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1539
Size: 262 1277
BsaI GGTCTCn'nnnn_
Cuts at: 0 915 1539
Size: 915 624
BsaAI yAC'GTr
Cuts at: 0 1116 1539
Size: 1116 423
BsaBI GATnn'nnATC
Cuts at: 0 550 1036 1539
Size: 550 486 503
Fragments arranged by size:
550 503 486
BsaHI Gr'CG_yC
Cuts at: 0 515 939 1539
Size: 515 424 600
Fragments arranged by size:
600 515 424
BsaWI w'CCGG_w
Cuts at: 0 345 1539
Size: 345 1194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 1539
Size: 448 30 1061
Fragments arranged by size:
1061 448 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 240 496 940 1318 1539
Size: 240 256 444 378 221
Fragments arranged by size:
444 378 256 240 221
BseYI C'CCAG_C
Cuts at: 0 363 1539
Size: 363 1176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 153 1297 1539
Size: 80 73 1144 242
Fragments arranged by size:
1144 242 80 73
BsiEI CG_ry'CG
Cuts at: 0 788 1539
Size: 788 751
BsiHKAI G_wGCw'C
Cuts at: 0 1166 1539
Size: 1166 373
BsmI GAATG_Cn'
Cuts at: 0 1463 1539
Size: 1463 76
Bsp1286I G_dGCh'C
Cuts at: 0 35 149 572 1166 1381 1539
Size: 35 114 423 594 215 158
Fragments arranged by size:
594 423 215 158 114 35
BspHI T'CATG_A
Cuts at: 0 518 1539
Size: 518 1021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 225 1334 1539
Size: 225 1109 205
Fragments arranged by size:
1109 225 205
BsrBI CCG'CTC
Cuts at: 0 230 592 792 990 1394 1539
Size: 230 362 200 198 404 145
Fragments arranged by size:
404 362 230 200 198 145
BsrDI GCAATG_nn'
Cuts at: 0 527 1539
Size: 527 1012
BsrGI T'GTAC_A
Cuts at: 0 962 1539
Size: 962 577
BstAPI GCAn_nnn'nTGC
Cuts at: 0 47 1539
Size: 47 1492
BstEII G'GTnAC_C
Cuts at: 0 84 1539
Size: 84 1455
BstYI r'GATC_y
Cuts at: 0 545 596 1018 1031 1109 1142 1539
Size: 545 51 422 13 78 33 397
Fragments arranged by size:
545 422 397 78 51 33 13
BtgI C'CryG_G
Cuts at: 0 5 534 680 740 1224 1539
Size: 5 529 146 60 484 315
Fragments arranged by size:
529 484 315 146 60 5
BtsI GCAGTG_nn'
Cuts at: 0 141 821 1529 1539
Size: 141 680 708 10
Fragments arranged by size:
708 680 141 10
ClaI AT'CG_AT
Cuts at: 0 296 1539
Size: 296 1243
EaeI y'GGCC_r
Cuts at: 0 2 444 537 770 974 1539
Size: 2 442 93 233 204 565
Fragments arranged by size:
565 442 233 204 93 2
EarI CTCTTCn'nnn_
Cuts at: 0 763 1539
Size: 763 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 436 1539
Size: 436 1103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1477 1539
Size: 1477 62
EcoO109I rG'GnC_Cy
Cuts at: 0 146 1323 1377 1378 1515 1539
Size: 146 1177 54 1 137 24
Fragments arranged by size:
1177 146 137 54 24 1
EcoRI G'AATT_C
Cuts at: 0 829 1339 1539
Size: 829 510 200
Fragments arranged by size:
829 510 200
EcoRV GAT'ATC
Cuts at: 0 438 1062 1539
Size: 438 624 477
Fragments arranged by size:
624 477 438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 738 1539
Size: 738 801
FspI TGC'GCA
Cuts at: 0 1435 1539
Size: 1435 104
HaeII r_GCGC'y
Cuts at: 0 817 942 1312 1510 1539
Size: 817 125 370 198 29
Fragments arranged by size:
817 370 198 125 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 576 608 1007 1039 1539
Size: 576 32 399 32 500
Fragments arranged by size:
576 500 399 32 32
KasI G'GCGC_C
Cuts at: 0 938 1539
Size: 938 601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1262 1539
Size: 1262 277
MscI TGG'CCA
Cuts at: 0 4 446 772 976 1539
Size: 4 442 326 204 563
Fragments arranged by size:
563 442 326 204 4
MslI CAynn'nnrTG
Cuts at: 0 110 685 1159 1229 1539
Size: 110 575 474 70 310
Fragments arranged by size:
575 474 310 110 70
MspA1I CmG'CkG
Cuts at: 0 536 1245 1539
Size: 536 709 294
Fragments arranged by size:
709 536 294
NarI GG'CG_CC
Cuts at: 0 939 1539
Size: 939 600
NcoI C'CATG_G
Cuts at: 0 5 680 740 1224 1539
Size: 5 675 60 484 315
Fragments arranged by size:
675 484 315 60 5
NspI r_CATG'y
Cuts at: 0 78 363 859 1539
Size: 78 285 496 680
Fragments arranged by size:
680 496 285 78
PfoI T'CCnGG_A
Cuts at: 0 951 1539
Size: 951 588
PpuMI rG'GwC_Cy
Cuts at: 0 1323 1515 1539
Size: 1323 192 24
Fragments arranged by size:
1323 192 24
PspOMI G'GGCC_C
Cuts at: 0 145 1377 1539
Size: 145 1232 162
Fragments arranged by size:
1232 162 145
PstI C_TGCA'G
Cuts at: 0 1349 1514 1539
Size: 1349 165 25
Fragments arranged by size:
1349 165 25
PvuI CG_AT'CG
Cuts at: 0 788 1539
Size: 788 751
PvuII CAG'CTG
Cuts at: 0 1245 1539
Size: 1245 294
SacII CC_GC'GG
Cuts at: 0 537 1539
Size: 537 1002
SfcI C'TryA_G
Cuts at: 0 120 1345 1510 1539
Size: 120 1225 165 29
Fragments arranged by size:
1225 165 120 29
SfoI GGC'GCC
Cuts at: 0 940 1539
Size: 940 599
SmlI C'TyrA_G
Cuts at: 0 241 1539
Size: 241 1298
StuI AGG'CCT
Cuts at: 0 24 898 1539
Size: 24 874 641
Fragments arranged by size:
874 641 24
StyI C'CwwG_G
Cuts at: 0 5 54 257 629 680 740 1224 1445
Size: 5 49 203 372 51 60 484 221
Cuts at: 1445 1539
Size: 94
Fragments arranged by size:
484 372 221 203 94 60 51 49
5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 1081 1539
Size: 118 963 458
Fragments arranged by size:
963 458 118
TatI w'GTAC_w
Cuts at: 0 962 1539
Size: 962 577
Tth111I GACn'n_nGTC
Cuts at: 0 166 1539
Size: 166 1373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 1539
Size: 636 903
XmnI GAAnn'nnTTC
Cuts at: 0 1363 1539
Size: 1363 176
ZraI GAC'GTC
Cuts at: 0 516 1539
Size: 516 1023
Enzymes that do cut:
AarI AatII AcuI AleI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BglI BglII
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI
BstEII BstYI BtgI BtsI ClaI EaeI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PfoI PpuMI PspOMI PstI PvuI PvuII SacII SfcI
SfoI SmlI StuI StyI TaqII TatI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BbvCI
BcgI BciVI BclI BfrBI BlpI BmtI BpmI Bpu10I
BsiWI BsmBI BspEI BsrFI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XhoI XmaI