Restriction map for
pFN21AE6480
(Linear) (Six-base) MAPSORT of: KIEE6480.seq Check: 7303 from: 1 to: 1530
KIEE6480.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AatII G_ACGT'C
Cuts at: 0 1069 1530
Size: 1069 461
AccI GT'mk_AC
Cuts at: 0 4 1530
Size: 4 1526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 556 868 1307 1530
Size: 85 471 312 439 223
Fragments arranged by size:
471 439 312 223 85
AflIII A'CryG_T
Cuts at: 0 827 1208 1524 1530
Size: 827 381 316 6
Fragments arranged by size:
827 381 316 6
AlwNI CAG_nnn'CTG
Cuts at: 0 706 1530
Size: 706 824
ApaI G_GGCC'C
Cuts at: 0 262 1530
Size: 262 1268
ApaLI G'TGCA_C
Cuts at: 0 127 1520 1530
Size: 127 1393 10
Fragments arranged by size:
1393 127 10
ApoI r'AATT_y
Cuts at: 0 190 1530
Size: 190 1340
AvaI C'yCGr_G
Cuts at: 0 757 1530
Size: 757 773
BanI G'GyrC_C
Cuts at: 0 293 586 1530
Size: 293 293 944
Fragments arranged by size:
944 293 293
BanII G_rGCy'C
Cuts at: 0 148 262 323 758 1530
Size: 148 114 61 435 772
Fragments arranged by size:
772 435 148 114 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 1143 1295 1530
Size: 856 287 152 235
Fragments arranged by size:
856 287 235 152
BbvCI CC'TCA_GC
Cuts at: 0 232 487 1530
Size: 232 255 1043
Fragments arranged by size:
1043 255 232
Bme1580I G_kGCm'C
Cuts at: 0 131 262 802 818 837 1524 1530
Size: 131 131 540 16 19 687 6
Fragments arranged by size:
687 540 131 131 19 16 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1530
Size: 582 948
Bpu10I CC'TnA_GC
Cuts at: 0 232 487 1530
Size: 232 255 1043
Fragments arranged by size:
1043 255 232
BsaI GGTCTCn'nnnn_
Cuts at: 0 763 1530
Size: 763 767
BsaAI yAC'GTr
Cuts at: 0 1211 1530
Size: 1211 319
BsaHI Gr'CG_yC
Cuts at: 0 1066 1112 1530
Size: 1066 46 418
Fragments arranged by size:
1066 418 46
BsaWI w'CCGG_w
Cuts at: 0 502 839 1530
Size: 502 337 691
Fragments arranged by size:
691 502 337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 1530
Size: 308 30 1192
Fragments arranged by size:
1192 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 105 781 1530
Size: 105 676 749
Fragments arranged by size:
749 676 105
BseYI C'CCAG_C
Cuts at: 0 338 1362 1530
Size: 338 1024 168
Fragments arranged by size:
1024 338 168
BsiHKAI G_wGCw'C
Cuts at: 0 113 131 1524 1530
Size: 113 18 1393 6
Fragments arranged by size:
1393 113 18 6
BsiWI C'GTAC_G
Cuts at: 0 1211 1530
Size: 1211 319
BsmI GAATG_Cn'
Cuts at: 0 573 1478 1530
Size: 573 905 52
Fragments arranged by size:
905 573 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 459 1530
Size: 459 1071
Bsp1286I G_dGCh'C
Cuts at: 0 113 131 148 262 323 758 802 818
Size: 113 18 17 114 61 435 44 16
Cuts at: 818 837 1524 1530
Size: 19 687 6
Fragments arranged by size:
687 435 114 113 61 44 19 18
17 16 6
BspEI T'CCGG_A
Cuts at: 0 839 1530
Size: 839 691
BspHI T'CATG_A
Cuts at: 0 12 623 1530
Size: 12 611 907
Fragments arranged by size:
907 611 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 434 440 791 1530
Size: 434 6 351 739
Fragments arranged by size:
739 434 351 6
BsrDI GCAATG_nn'
Cuts at: 0 1357 1530
Size: 1357 173
BstBI TT'CG_AA
Cuts at: 0 1482 1530
Size: 1482 48
BstYI r'GATC_y
Cuts at: 0 1385 1530
Size: 1385 145
BstZ17I GTA'TAC
Cuts at: 0 5 1530
Size: 5 1525
BtgI C'CryG_G
Cuts at: 0 204 494 1530
Size: 204 290 1036
Fragments arranged by size:
1036 290 204
EaeI y'GGCC_r
Cuts at: 0 1160 1313 1530
Size: 1160 153 217
Fragments arranged by size:
1160 217 153
EarI CTCTTCn'nnn_
Cuts at: 0 382 457 734 1530
Size: 382 75 277 796
Fragments arranged by size:
796 382 277 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 1530
Size: 104 1426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 556 582 868 1307 1530
Size: 85 471 26 286 439 223
Fragments arranged by size:
471 439 286 223 85 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 1376 1530
Size: 478 898 154
Fragments arranged by size:
898 478 154
EcoO109I rG'GnC_Cy
Cuts at: 0 259 483 629 1530
Size: 259 224 146 901
Fragments arranged by size:
901 259 224 146
EcoRI G'AATT_C
Cuts at: 0 190 1530
Size: 190 1340
FspI TGC'GCA
Cuts at: 0 549 1530
Size: 549 981
HaeII r_GCGC'y
Cuts at: 0 622 1530
Size: 622 908
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 308 340 458 490 909 941 1530
Size: 308 32 118 32 419 32 589
Fragments arranged by size:
589 419 308 118 32 32 32
HincII GTy'rAC
Cuts at: 0 327 716 1530
Size: 327 389 814
Fragments arranged by size:
814 389 327
MscI TGG'CCA
Cuts at: 0 1162 1315 1530
Size: 1162 153 215
Fragments arranged by size:
1162 215 153
MspA1I CmG'CkG
Cuts at: 0 206 400 413 433 860 1530
Size: 206 194 13 20 427 670
Fragments arranged by size:
670 427 206 194 20 13
NdeI CA'TA_TG
Cuts at: 0 1426 1530
Size: 1426 104
NspI r_CATG'y
Cuts at: 0 831 1528 1530
Size: 831 697 2
Fragments arranged by size:
831 697 2
PasI CC'CwG_GG
Cuts at: 0 159 333 480 1530
Size: 159 174 147 1050
Fragments arranged by size:
1050 174 159 147
PciI A'CATG_T
Cuts at: 0 827 1524 1530
Size: 827 697 6
Fragments arranged by size:
827 697 6
PspOMI G'GGCC_C
Cuts at: 0 258 1530
Size: 258 1272
PvuII CAG'CTG
Cuts at: 0 400 860 1530
Size: 400 460 670
Fragments arranged by size:
670 460 400
SacII CC_GC'GG
Cuts at: 0 207 1530
Size: 207 1323
StyI C'CwwG_G
Cuts at: 0 582 1530
Size: 582 948
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 89 151 281 330 1530
Size: 89 62 130 49 1200
Fragments arranged by size:
1200 130 89 62 49
XmnI GAAnn'nnTTC
Cuts at: 0 886 1150 1530
Size: 886 264 380
Fragments arranged by size:
886 380 264
ZraI GAC'GTC
Cuts at: 0 1067 1530
Size: 1067 463
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI Bme1580I BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BstBI BstYI BstZ17I BtgI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII MscI
MspA1I NdeI NspI PasI PciI PspOMI PvuII SacII
StyI TaqII XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaBI BsgI BsiEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRV FalI FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI