Restriction map for
pFN21AE6478
(Linear) (Six-base) MAPSORT of: KIEE6478.seq Check: 7865 from: 1 to: 1449
KIEE6478.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1449
Size: 110 1339
AfeI AGC'GCT
Cuts at: 0 331 1449
Size: 331 1118
AhdI GACnn_n'nnGTC
Cuts at: 0 60 1449
Size: 60 1389
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 1449
Size: 1386 63
ApaI G_GGCC'C
Cuts at: 0 262 1449
Size: 262 1187
ApaLI G'TGCA_C
Cuts at: 0 1053 1449
Size: 1053 396
AvaI C'yCGr_G
Cuts at: 0 1112 1449
Size: 1112 337
BamHI G'GATC_C
Cuts at: 0 212 1449
Size: 212 1237
BanI G'GyrC_C
Cuts at: 0 1243 1449
Size: 1243 206
BanII G_rGCy'C
Cuts at: 0 262 1234 1312 1449
Size: 262 972 78 137
Fragments arranged by size:
972 262 137 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 793 1415 1449
Size: 154 639 622 34
Fragments arranged by size:
639 622 154 34
BclI T'GATC_A
Cuts at: 0 1337 1449
Size: 1337 112
BfrBI ATG'CAT
Cuts at: 0 384 1449
Size: 384 1065
Bme1580I G_kGCm'C
Cuts at: 0 208 262 327 1057 1134 1449
Size: 208 54 65 730 77 315
Fragments arranged by size:
730 315 208 77 65 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 441 1449
Size: 441 1008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1297 1449
Size: 400 897 152
Fragments arranged by size:
897 400 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1384 1449
Size: 1384 65
BsaBI GATnn'nnATC
Cuts at: 0 1342 1449
Size: 1342 107
BsaHI Gr'CG_yC
Cuts at: 0 1331 1449
Size: 1331 118
BsaWI w'CCGG_w
Cuts at: 0 653 1449
Size: 653 796
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 1449
Size: 601 30 818
Fragments arranged by size:
818 601 30
BseYI C'CCAG_C
Cuts at: 0 326 757 885 1127 1449
Size: 326 431 128 242 322
Fragments arranged by size:
431 326 322 242 128
BsiHKAI G_wGCw'C
Cuts at: 0 1057 1449
Size: 1057 392
BsmI GAATG_Cn'
Cuts at: 0 876 1449
Size: 876 573
BsmBI CGTCTCn'nnnn_
Cuts at: 0 565 1449
Size: 565 884
Bsp1286I G_dGCh'C
Cuts at: 0 208 262 327 1057 1134 1234 1312 1449
Size: 208 54 65 730 77 100 78 137
Fragments arranged by size:
730 208 137 100 78 77 65 54
BspEI T'CCGG_A
Cuts at: 0 653 1449
Size: 653 796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 962 1004 1449
Size: 962 42 445
Fragments arranged by size:
962 445 42
BsrBI CCG'CTC
Cuts at: 0 1272 1449
Size: 1272 177
BsrDI GCAATG_nn'
Cuts at: 0 287 1037 1449
Size: 287 750 412
Fragments arranged by size:
750 412 287
BsrFI r'CCGG_y
Cuts at: 0 761 1042 1449
Size: 761 281 407
Fragments arranged by size:
761 407 281
BsrGI T'GTAC_A
Cuts at: 0 491 1449
Size: 491 958
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1052 1449
Size: 1052 397
BstEII G'GTnAC_C
Cuts at: 0 1281 1449
Size: 1281 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 87 1449
Size: 51 36 1362
Fragments arranged by size:
1362 51 36
BstYI r'GATC_y
Cuts at: 0 212 665 1143 1449
Size: 212 453 478 306
Fragments arranged by size:
478 453 306 212
Bsu36I CC'TnA_GG
Cuts at: 0 661 1449
Size: 661 788
BtgI C'CryG_G
Cuts at: 0 593 1449
Size: 593 856
BtsI GCAGTG_nn'
Cuts at: 0 1132 1449
Size: 1132 317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1422 1449
Size: 1422 27
DraI TTT'AAA
Cuts at: 0 518 1449
Size: 518 931
DraIII CAC_nnn'GTG
Cuts at: 0 189 1449
Size: 189 1260
EaeI y'GGCC_r
Cuts at: 0 689 1187 1449
Size: 689 498 262
Fragments arranged by size:
689 498 262
EarI CTCTTCn'nnn_
Cuts at: 0 805 1449
Size: 805 644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 400 1297 1449
Size: 110 290 897 152
Fragments arranged by size:
897 290 152 110
EcoO109I rG'GnC_Cy
Cuts at: 0 25 1373 1383 1449
Size: 25 1348 10 66
Fragments arranged by size:
1348 66 25 10
HaeII r_GCGC'y
Cuts at: 0 333 1449
Size: 333 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 212 244 780 812 956 988
Size: 46 32 134 32 536 32 144 32
Cuts at: 988 1449
Size: 461
Fragments arranged by size:
536 461 144 134 46 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1121 1449
Size: 1121 328
HindIII A'AGCT_T
Cuts at: 0 1027 1449
Size: 1027 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1449
Size: 68 1381
MscI TGG'CCA
Cuts at: 0 691 1189 1449
Size: 691 498 260
Fragments arranged by size:
691 498 260
MslI CAynn'nnrTG
Cuts at: 0 163 253 379 700 838 934 1449
Size: 163 90 126 321 138 96 515
Fragments arranged by size:
515 321 163 138 126 96 90
NaeI GCC'GGC
Cuts at: 0 1044 1449
Size: 1044 405
NcoI C'CATG_G
Cuts at: 0 593 1449
Size: 593 856
NgoMIV G'CCGG_C
Cuts at: 0 1042 1449
Size: 1042 407
NruI TCG'CGA
Cuts at: 0 603 1449
Size: 603 846
NsiI A_TGCA'T
Cuts at: 0 386 1449
Size: 386 1063
NspI r_CATG'y
Cuts at: 0 615 705 1181 1449
Size: 615 90 476 268
Fragments arranged by size:
615 476 268 90
PasI CC'CwG_GG
Cuts at: 0 30 1449
Size: 30 1419
PflMI CCAn_nnn'nTGG
Cuts at: 0 410 1386 1449
Size: 410 976 63
Fragments arranged by size:
976 410 63
PfoI T'CCnGG_A
Cuts at: 0 1274 1449
Size: 1274 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1449
Size: 85 32 1332
Fragments arranged by size:
1332 85 32
PpuMI rG'GwC_Cy
Cuts at: 0 25 1373 1449
Size: 25 1348 76
Fragments arranged by size:
1348 76 25
PspOMI G'GGCC_C
Cuts at: 0 258 1449
Size: 258 1191
PstI C_TGCA'G
Cuts at: 0 1070 1086 1449
Size: 1070 16 363
Fragments arranged by size:
1070 363 16
SanDI GG'GwC_CC
Cuts at: 0 25 1449
Size: 25 1424
ScaI AGT'ACT
Cuts at: 0 18 1449
Size: 18 1431
SexAI A'CCwGG_T
Cuts at: 0 185 1211 1449
Size: 185 1026 238
Fragments arranged by size:
1026 238 185
SfcI C'TryA_G
Cuts at: 0 1066 1082 1449
Size: 1066 16 367
Fragments arranged by size:
1066 367 16
SmaI CCC'GGG
Cuts at: 0 1114 1449
Size: 1114 335
SmlI C'TyrA_G
Cuts at: 0 1399 1449
Size: 1399 50
SphI G_CATG'C
Cuts at: 0 705 1449
Size: 705 744
StuI AGG'CCT
Cuts at: 0 175 1449
Size: 175 1274
StyI C'CwwG_G
Cuts at: 0 152 593 1449
Size: 152 441 856
Fragments arranged by size:
856 441 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 1449
Size: 484 965
TatI w'GTAC_w
Cuts at: 0 16 491 731 1449
Size: 16 475 240 718
Fragments arranged by size:
718 475 240 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 1449
Size: 1408 32 9
Fragments arranged by size:
1408 32 9
Tth111I GACn'n_nGTC
Cuts at: 0 1213 1449
Size: 1213 236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 897 1449
Size: 897 552
XmaI C'CCGG_G
Cuts at: 0 1112 1449
Size: 1112 337
Enzymes that do cut:
AcuI AfeI AhdI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbsI BclI BfrBI Bme1580I BmrI BpmI
BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII MmeI MscI MslI NaeI NcoI NgoMIV
NruI NsiI NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI SanDI ScaI SexAI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BseRI BsgI
BsiEI BsiWI BspHI BssHII BssSI BstBI BstZ17I BtgZI
ClaI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MspA1I NarI NdeI NheI NotI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmnI ZraI