Restriction map for
pFN21AE6473
(Linear) (Six-base) MAPSORT of: KIEE6473.seq Check: 9708 from: 1 to: 1359
KIEE6473.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 341 1359
Size: 341 1018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1147 1359
Size: 403 744 212
Fragments arranged by size:
744 403 212
AlwNI CAG_nnn'CTG
Cuts at: 0 291 350 1092 1255 1359
Size: 291 59 742 163 104
Fragments arranged by size:
742 291 163 104 59
AvaI C'yCGr_G
Cuts at: 0 64 637 671 1359
Size: 64 573 34 688
Fragments arranged by size:
688 573 64 34
AvrII C'CTAG_G
Cuts at: 0 1240 1359
Size: 1240 119
BanI G'GyrC_C
Cuts at: 0 168 1359
Size: 168 1191
BanII G_rGCy'C
Cuts at: 0 548 1061 1359
Size: 548 513 298
Fragments arranged by size:
548 513 298
BbeI G_GCGC'C
Cuts at: 0 172 1359
Size: 172 1187
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 1359
Size: 366 993
BbvCI CC'TCA_GC
Cuts at: 0 437 479 1359
Size: 437 42 880
Fragments arranged by size:
880 437 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 1161 1359
Size: 1161 198
BglI GCCn_nnn'nGGC
Cuts at: 0 811 976 1359
Size: 811 165 383
Fragments arranged by size:
811 383 165
BlpI GC'TnA_GC
Cuts at: 0 181 547 1359
Size: 181 366 812
Fragments arranged by size:
812 366 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 1359
Size: 153 1206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1159 1359
Size: 680 479 200
Fragments arranged by size:
680 479 200
Bpu10I CC'TnA_GC
Cuts at: 0 437 479 1359
Size: 437 42 880
Fragments arranged by size:
880 437 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 838 1294 1359
Size: 376 462 456 65
Fragments arranged by size:
462 456 376 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 696 854 1359
Size: 35 661 158 505
Fragments arranged by size:
661 505 158 35
BsaAI yAC'GTr
Cuts at: 0 682 1359
Size: 682 677
BsaHI Gr'CG_yC
Cuts at: 0 169 1359
Size: 169 1190
BsaWI w'CCGG_w
Cuts at: 0 929 1359
Size: 929 430
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 1289 1319 1359
Size: 76 30 1183 30 40
Fragments arranged by size:
1183 76 40 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 97 132 454 496 507 1359
Size: 88 9 35 322 42 11 852
Fragments arranged by size:
852 322 88 42 35 11 9
BseYI C'CCAG_C
Cuts at: 0 30 264 414 979 1359
Size: 30 234 150 565 380
Fragments arranged by size:
565 380 234 150 30
BsiHKAI G_wGCw'C
Cuts at: 0 548 993 1061 1359
Size: 548 445 68 298
Fragments arranged by size:
548 445 298 68
Bsp1286I G_dGCh'C
Cuts at: 0 548 993 1061 1359
Size: 548 445 68 298
Fragments arranged by size:
548 445 298 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 1359
Size: 299 1060
BsrBI CCG'CTC
Cuts at: 0 15 1359
Size: 15 1344
BssSI C'ACGA_G
Cuts at: 0 1039 1359
Size: 1039 320
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 1359
Size: 291 1068
BstYI r'GATC_y
Cuts at: 0 353 932 1049 1188 1244 1359
Size: 353 579 117 139 56 115
Fragments arranged by size:
579 353 139 117 115 56
BtgI C'CryG_G
Cuts at: 0 275 527 1359
Size: 275 252 832
Fragments arranged by size:
832 275 252
DraIII CAC_nnn'GTG
Cuts at: 0 528 1359
Size: 528 831
EaeI y'GGCC_r
Cuts at: 0 272 332 1359
Size: 272 60 1027
Fragments arranged by size:
1027 272 60
EarI CTCTTCn'nnn_
Cuts at: 0 616 1359
Size: 616 743
EcoICRI GAG'CTC
Cuts at: 0 546 1059 1359
Size: 546 513 300
Fragments arranged by size:
546 513 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 680 1147 1159 1359
Size: 403 277 467 12 200
Fragments arranged by size:
467 403 277 200 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1162 1359
Size: 1162 197
EcoO109I rG'GnC_Cy
Cuts at: 0 27 288 444 584 1359
Size: 27 261 156 140 775
Fragments arranged by size:
775 261 156 140 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 1288 1359
Size: 177 1111 71
Fragments arranged by size:
1111 177 71
HaeII r_GCGC'y
Cuts at: 0 172 1359
Size: 172 1187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 757 789 1267 1289 1299 1321
Size: 76 32 649 32 478 22 10 22
Cuts at: 1321 1359
Size: 38
Fragments arranged by size:
649 478 76 38 32 32 22 22
10
KasI G'GCGC_C
Cuts at: 0 168 1359
Size: 168 1191
MfeI C'AATT_G
Cuts at: 0 1333 1359
Size: 1333 26
MscI TGG'CCA
Cuts at: 0 274 334 1359
Size: 274 60 1025
Fragments arranged by size:
1025 274 60
MspA1I CmG'CkG
Cuts at: 0 264 315 350 436 609 729 837 1359
Size: 264 51 35 86 173 120 108 522
Fragments arranged by size:
522 264 173 120 108 86 51 35
NarI GG'CG_CC
Cuts at: 0 169 1359
Size: 169 1190
NruI TCG'CGA
Cuts at: 0 125 1359
Size: 125 1234
NspI r_CATG'y
Cuts at: 0 93 414 818 898 1359
Size: 93 321 404 80 461
Fragments arranged by size:
461 404 321 93 80
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 528 1359
Size: 204 324 831
Fragments arranged by size:
831 324 204
PfoI T'CCnGG_A
Cuts at: 0 923 1359
Size: 923 436
PmlI CAC'GTG
Cuts at: 0 682 1359
Size: 682 677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 734 766 1359
Size: 734 32 593
Fragments arranged by size:
734 593 32
PpuMI rG'GwC_Cy
Cuts at: 0 288 444 584 1359
Size: 288 156 140 775
Fragments arranged by size:
775 288 156 140
PspXI vC'TCGA_Gb
Cuts at: 0 637 1359
Size: 637 722
PstI C_TGCA'G
Cuts at: 0 287 314 608 614 1359
Size: 287 27 294 6 745
Fragments arranged by size:
745 294 287 27 6
PvuII CAG'CTG
Cuts at: 0 350 436 609 729 837 1359
Size: 350 86 173 120 108 522
Fragments arranged by size:
522 350 173 120 108 86
SacI G_AGCT'C
Cuts at: 0 548 1061 1359
Size: 548 513 298
Fragments arranged by size:
548 513 298
SfcI C'TryA_G
Cuts at: 0 283 310 604 610 1359
Size: 283 27 294 6 749
Fragments arranged by size:
749 294 283 27 6
SfoI GGC'GCC
Cuts at: 0 170 1359
Size: 170 1189
SmlI C'TyrA_G
Cuts at: 0 391 637 853 1273 1359
Size: 391 246 216 420 86
Fragments arranged by size:
420 391 246 216 86
SphI G_CATG'C
Cuts at: 0 818 898 1359
Size: 818 80 461
Fragments arranged by size:
818 461 80
StuI AGG'CCT
Cuts at: 0 238 889 1359
Size: 238 651 470
Fragments arranged by size:
651 470 238
StyI C'CwwG_G
Cuts at: 0 686 1240 1359
Size: 686 554 119
Fragments arranged by size:
686 554 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1084 1359
Size: 1084 275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1182 1359
Size: 1182 177
XhoI C'TCGA_G
Cuts at: 0 637 1359
Size: 637 722
XmnI GAAnn'nnTTC
Cuts at: 0 746 1359
Size: 746 613
Enzymes that do cut:
AccI AcuI AlwNI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BciVI BglI BlpI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BssSI BstAPI BstYI BtgI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MfeI MscI MspA1I NarI NruI
NspI PflMI PfoI PmlI PpiI PpuMI PspXI PstI
PvuII SacI SfcI SfoI SmlI SphI StuI StyI
TaqII XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BcgI BclI BfrBI BglII
Bme1580I BmgBI BmtI BplI BsaBI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MluI MmeI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NsiI
PacI PasI PciI PmeI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XmaI ZraI