Restriction map for
pFN21AE6470
(Linear) (Six-base) MAPSORT of: KIEE6470.seq Check: 4438 from: 1 to: 1512
KIEE6470.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AclI AA'CG_TT
Cuts at: 0 719 1512
Size: 719 793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1512
Size: 415 1097
ApoI r'AATT_y
Cuts at: 0 90 1055 1141 1171 1307 1476 1482 1512
Size: 90 965 86 30 136 169 6 30
Fragments arranged by size:
965 169 136 90 86 30 30 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 824 857 1512
Size: 824 33 655
Fragments arranged by size:
824 655 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 421 507 663 1512
Size: 421 86 156 849
Fragments arranged by size:
849 421 156 86
BclI T'GATC_A
Cuts at: 0 162 1512
Size: 162 1350
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1512
Size: 34 1478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1397 1512
Size: 577 820 115
Fragments arranged by size:
820 577 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1512
Size: 896 616
BsaBI GATnn'nnATC
Cuts at: 0 1120 1512
Size: 1120 392
BsaHI Gr'CG_yC
Cuts at: 0 16 1512
Size: 16 1496
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1512
Size: 61 1451
BseYI C'CCAG_C
Cuts at: 0 489 1512
Size: 489 1023
BsmI GAATG_Cn'
Cuts at: 0 1369 1512
Size: 1369 143
BspHI T'CATG_A
Cuts at: 0 543 1512
Size: 543 969
BsrFI r'CCGG_y
Cuts at: 0 18 1512
Size: 18 1494
BsrGI T'GTAC_A
Cuts at: 0 183 1512
Size: 183 1329
BstBI TT'CG_AA
Cuts at: 0 1321 1512
Size: 1321 191
BstYI r'GATC_y
Cuts at: 0 777 1183 1512
Size: 777 406 329
Fragments arranged by size:
777 406 329
BtgI C'CryG_G
Cuts at: 0 725 1125 1512
Size: 725 400 387
Fragments arranged by size:
725 400 387
DraI TTT'AAA
Cuts at: 0 363 1016 1512
Size: 363 653 496
Fragments arranged by size:
653 496 363
EaeI y'GGCC_r
Cuts at: 0 1280 1512
Size: 1280 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 468 1512
Size: 468 1044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 577 1397 1512
Size: 415 162 820 115
Fragments arranged by size:
820 415 162 115
EcoO109I rG'GnC_Cy
Cuts at: 0 71 1512
Size: 71 1441
EcoRI G'AATT_C
Cuts at: 0 1482 1512
Size: 1482 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 1512
Size: 88 32 1392
Fragments arranged by size:
1392 88 32
HincII GTy'rAC
Cuts at: 0 371 465 621 1512
Size: 371 94 156 891
Fragments arranged by size:
891 371 156 94
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1236 1512
Size: 787 449 276
Fragments arranged by size:
787 449 276
MscI TGG'CCA
Cuts at: 0 1282 1512
Size: 1282 230
MslI CAynn'nnrTG
Cuts at: 0 862 1078 1512
Size: 862 216 434
Fragments arranged by size:
862 434 216
MspA1I CmG'CkG
Cuts at: 0 489 1512
Size: 489 1023
NaeI GCC'GGC
Cuts at: 0 20 1512
Size: 20 1492
NcoI C'CATG_G
Cuts at: 0 725 1125 1512
Size: 725 400 387
Fragments arranged by size:
725 400 387
NgoMIV G'CCGG_C
Cuts at: 0 18 1512
Size: 18 1494
NspI r_CATG'y
Cuts at: 0 774 1493 1512
Size: 774 719 19
Fragments arranged by size:
774 719 19
PfoI T'CCnGG_A
Cuts at: 0 554 1512
Size: 554 958
PsiI TTA'TAA
Cuts at: 0 658 1334 1512
Size: 658 676 178
Fragments arranged by size:
676 658 178
PvuII CAG'CTG
Cuts at: 0 489 1512
Size: 489 1023
SfcI C'TryA_G
Cuts at: 0 498 1364 1512
Size: 498 866 148
Fragments arranged by size:
866 498 148
SgrAI Cr'CCGG_yG
Cuts at: 0 18 1512
Size: 18 1494
SmlI C'TyrA_G
Cuts at: 0 911 1512
Size: 911 601
SspI AAT'ATT
Cuts at: 0 216 924 1359 1512
Size: 216 708 435 153
Fragments arranged by size:
708 435 216 153
StyI C'CwwG_G
Cuts at: 0 418 725 1125 1512
Size: 418 307 400 387
Fragments arranged by size:
418 400 387 307
TatI w'GTAC_w
Cuts at: 0 183 699 1512
Size: 183 516 813
Fragments arranged by size:
813 516 183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 1512
Size: 723 789
XmnI GAAnn'nnTTC
Cuts at: 0 303 1512
Size: 303 1209
Enzymes that do cut:
AclI AcuI ApoI BaeI BbsI BclI BglI BpmI
BpuEI BsaBI BsaHI BseRI BseYI BsmI BspHI BsrFI
BsrGI BstBI BstYI BtgI DraI EaeI EciI Eco57MI
EcoO109I EcoRI Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PfoI PsiI PvuII SfcI
SgrAI SmlI SspI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaWI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EarI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI