Restriction map for
pFN21AE6469
(Linear) (Six-base) MAPSORT of: KIEE6469.seq Check: 7258 from: 1 to: 1590
KIEE6469.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 142 1590
Size: 142 1448
AatII G_ACGT'C
Cuts at: 0 260 1590
Size: 260 1330
AccI GT'mk_AC
Cuts at: 0 1057 1590
Size: 1057 533
AclI AA'CG_TT
Cuts at: 0 701 1590
Size: 701 889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 850 1277 1590
Size: 380 470 427 313
Fragments arranged by size:
470 427 380 313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 1590
Size: 202 1388
ApaI G_GGCC'C
Cuts at: 0 48 1590
Size: 48 1542
ApoI r'AATT_y
Cuts at: 0 203 793 999 1590
Size: 203 590 206 591
Fragments arranged by size:
591 590 206 203
AseI AT'TA_AT
Cuts at: 0 875 1590
Size: 875 715
AvaI C'yCGr_G
Cuts at: 0 80 1590
Size: 80 1510
BanI G'GyrC_C
Cuts at: 0 124 643 1590
Size: 124 519 947
Fragments arranged by size:
947 519 124
BanII G_rGCy'C
Cuts at: 0 48 1299 1590
Size: 48 1251 291
Fragments arranged by size:
1251 291 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 546 1571 1590
Size: 546 1025 19
Fragments arranged by size:
1025 546 19
BbvCI CC'TCA_GC
Cuts at: 0 32 1590
Size: 32 1558
BfrBI ATG'CAT
Cuts at: 0 682 1590
Size: 682 908
BglII A'GATC_T
Cuts at: 0 1011 1590
Size: 1011 579
BlpI GC'TnA_GC
Cuts at: 0 1403 1590
Size: 1403 187
Bme1580I G_kGCm'C
Cuts at: 0 48 648 1590
Size: 48 600 942
Fragments arranged by size:
942 600 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 750 1590
Size: 750 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1590
Size: 1247 343
Bpu10I CC'TnA_GC
Cuts at: 0 32 248 1590
Size: 32 216 1342
Fragments arranged by size:
1342 216 32
BsaHI Gr'CG_yC
Cuts at: 0 257 1590
Size: 257 1333
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1105 1590
Size: 1105 485
BseYI C'CCAG_C
Cuts at: 0 1570 1590
Size: 1570 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 325 1590
Size: 170 155 1265
Fragments arranged by size:
1265 170 155
BsiHKAI G_wGCw'C
Cuts at: 0 849 1299 1590
Size: 849 450 291
Fragments arranged by size:
849 450 291
BsmI GAATG_Cn'
Cuts at: 0 1365 1590
Size: 1365 225
Bsp1286I G_dGCh'C
Cuts at: 0 48 648 849 1299 1590
Size: 48 600 201 450 291
Fragments arranged by size:
600 450 291 201 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 1590
Size: 142 1448
BsrDI GCAATG_nn'
Cuts at: 0 186 1256 1590
Size: 186 1070 334
Fragments arranged by size:
1070 334 186
BsrGI T'GTAC_A
Cuts at: 0 323 1590
Size: 323 1267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 1471 1590
Size: 96 1375 119
Fragments arranged by size:
1375 119 96
BstEII G'GTnAC_C
Cuts at: 0 547 1590
Size: 547 1043
BstYI r'GATC_y
Cuts at: 0 1011 1590
Size: 1011 579
BstZ17I GTA'TAC
Cuts at: 0 1058 1590
Size: 1058 532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1226 1261 1590
Size: 1226 35 329
Fragments arranged by size:
1226 329 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1040 1590
Size: 1040 550
EaeI y'GGCC_r
Cuts at: 0 992 1590
Size: 992 598
EarI CTCTTCn'nnn_
Cuts at: 0 568 755 941 1287 1590
Size: 568 187 186 346 303
Fragments arranged by size:
568 346 303 187 186
EcoICRI GAG'CTC
Cuts at: 0 1297 1590
Size: 1297 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 850 1247 1277 1590
Size: 380 470 397 30 313
Fragments arranged by size:
470 397 380 313 30
EcoO109I rG'GnC_Cy
Cuts at: 0 115 336 1590
Size: 115 221 1254
Fragments arranged by size:
1254 221 115
EcoRI G'AATT_C
Cuts at: 0 999 1590
Size: 999 591
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 854 886 1566 1590
Size: 854 32 680 24
Fragments arranged by size:
854 680 32 24
HincII GTy'rAC
Cuts at: 0 21 1101 1590
Size: 21 1080 489
Fragments arranged by size:
1080 489 21
HindIII A'AGCT_T
Cuts at: 0 1313 1590
Size: 1313 277
HpaI GTT'AAC
Cuts at: 0 1101 1590
Size: 1101 489
MfeI C'AATT_G
Cuts at: 0 1238 1590
Size: 1238 352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1590
Size: 238 1352
MslI CAynn'nnrTG
Cuts at: 0 133 1590
Size: 133 1457
NsiI A_TGCA'T
Cuts at: 0 684 1590
Size: 684 906
NspI r_CATG'y
Cuts at: 0 1039 1411 1590
Size: 1039 372 179
Fragments arranged by size:
1039 372 179
PfoI T'CCnGG_A
Cuts at: 0 1224 1590
Size: 1224 366
PpuMI rG'GwC_Cy
Cuts at: 0 115 1590
Size: 115 1475
PsiI TTA'TAA
Cuts at: 0 749 1590
Size: 749 841
PspOMI G'GGCC_C
Cuts at: 0 44 1590
Size: 44 1546
PstI C_TGCA'G
Cuts at: 0 92 759 1590
Size: 92 667 831
Fragments arranged by size:
831 667 92
SacI G_AGCT'C
Cuts at: 0 1299 1590
Size: 1299 291
SapI GCTCTTCn'nnn_
Cuts at: 0 941 1287 1590
Size: 941 346 303
Fragments arranged by size:
941 346 303
SexAI A'CCwGG_T
Cuts at: 0 543 1590
Size: 543 1047
SfcI C'TryA_G
Cuts at: 0 88 755 1019 1590
Size: 88 667 264 571
Fragments arranged by size:
667 571 264 88
StyI C'CwwG_G
Cuts at: 0 26 621 1343 1590
Size: 26 595 722 247
Fragments arranged by size:
722 595 247 26
TatI w'GTAC_w
Cuts at: 0 183 323 810 1590
Size: 183 140 487 780
Fragments arranged by size:
780 487 183 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 980 1012 1590
Size: 980 32 578
Fragments arranged by size:
980 578 32
Tth111I GACn'n_nGTC
Cuts at: 0 838 1590
Size: 838 752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 1545 1590
Size: 33 1512 45
Fragments arranged by size:
1512 45 33
ZraI GAC'GTC
Cuts at: 0 258 1590
Size: 258 1332
Enzymes that do cut:
AarI AatII AccI AclI AcuI AlfI ApaI ApoI
AseI AvaI BanI BanII BbsI BbvCI BfrBI BglII
BlpI Bme1580I BplI BpmI Bpu10I BsaHI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstAPI
BstEII BstYI BstZ17I CspCI DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI MfeI
MmeI MslI NsiI NspI PfoI PpuMI PsiI PspOMI
PstI SacI SapI SexAI SfcI StyI TatI TstI
Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AhdI AleI AloI
AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BglI BmgBI BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstXI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EagI
EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII
KasI KpnI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII XbaI XhoI XmaI
XmnI