Restriction map for
pFN21AE6467
(Linear) (Six-base) MAPSORT of: KIEE6467.seq Check: 6185 from: 1 to: 1545
KIEE6467.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 834 1545
Size: 834 711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1545
Size: 281 1264
AflIII A'CryG_T
Cuts at: 0 432 1545
Size: 432 1113
AleI CACnn'nnGTG
Cuts at: 0 1042 1545
Size: 1042 503
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 893 925 1545
Size: 893 32 620
Fragments arranged by size:
893 620 32
AlwNI CAG_nnn'CTG
Cuts at: 0 598 1545
Size: 598 947
ApoI r'AATT_y
Cuts at: 0 279 329 622 1235 1545
Size: 279 50 293 613 310
Fragments arranged by size:
613 310 293 279 50
AseI AT'TA_AT
Cuts at: 0 751 1545
Size: 751 794
BanII G_rGCy'C
Cuts at: 0 1455 1545
Size: 1455 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 935 1545
Size: 449 486 610
Fragments arranged by size:
610 486 449
BclI T'GATC_A
Cuts at: 0 1433 1545
Size: 1433 112
BfrBI ATG'CAT
Cuts at: 0 3 1545
Size: 3 1542
BmtI G_CTAG'C
Cuts at: 0 764 1545
Size: 764 781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1545
Size: 1046 499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 1545
Size: 828 717
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1145 1175 1545
Size: 1145 30 370
Fragments arranged by size:
1145 370 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 197 1545
Size: 53 144 1348
Fragments arranged by size:
1348 144 53
BseYI C'CCAG_C
Cuts at: 0 502 1545
Size: 502 1043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 611 1545
Size: 77 534 934
Fragments arranged by size:
934 534 77
BsiHKAI G_wGCw'C
Cuts at: 0 1455 1545
Size: 1455 90
BsmI GAATG_Cn'
Cuts at: 0 737 1466 1545
Size: 737 729 79
Fragments arranged by size:
737 729 79
Bsp1286I G_dGCh'C
Cuts at: 0 1455 1545
Size: 1455 90
BsrDI GCAATG_nn'
Cuts at: 0 473 640 1055 1545
Size: 473 167 415 490
Fragments arranged by size:
490 473 415 167
BstBI TT'CG_AA
Cuts at: 0 1233 1545
Size: 1233 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1009 1545
Size: 1009 536
BstYI r'GATC_y
Cuts at: 0 1021 1545
Size: 1021 524
BstZ17I GTA'TAC
Cuts at: 0 835 1545
Size: 835 710
Bsu36I CC'TnA_GG
Cuts at: 0 825 1545
Size: 825 720
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1157 1545
Size: 1157 388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 509 544 1545
Size: 509 35 1001
Fragments arranged by size:
1001 509 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1071 1545
Size: 1071 474
EarI CTCTTCn'nnn_
Cuts at: 0 380 946 1545
Size: 380 566 599
Fragments arranged by size:
599 566 380
EcoICRI GAG'CTC
Cuts at: 0 1453 1545
Size: 1453 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1046 1545
Size: 281 765 499
Fragments arranged by size:
765 499 281
EcoNI CCTnn'n_nnAGG
Cuts at: 0 179 580 858 1545
Size: 179 401 278 687
Fragments arranged by size:
687 401 278 179
EcoO109I rG'GnC_Cy
Cuts at: 0 1342 1474 1545
Size: 1342 132 71
Fragments arranged by size:
1342 132 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1425 1457 1545
Size: 1425 32 88
Fragments arranged by size:
1425 88 32
HincII GTy'rAC
Cuts at: 0 1529 1545
Size: 1529 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1131 1545
Size: 766 365 414
Fragments arranged by size:
766 414 365
MslI CAynn'nnrTG
Cuts at: 0 437 700 1042 1545
Size: 437 263 342 503
Fragments arranged by size:
503 437 342 263
MspA1I CmG'CkG
Cuts at: 0 595 1545
Size: 595 950
NdeI CA'TA_TG
Cuts at: 0 1392 1545
Size: 1392 153
NheI G'CTAG_C
Cuts at: 0 760 1545
Size: 760 785
NsiI A_TGCA'T
Cuts at: 0 5 1545
Size: 5 1540
NspI r_CATG'y
Cuts at: 0 436 806 1545
Size: 436 370 739
Fragments arranged by size:
739 436 370
PciI A'CATG_T
Cuts at: 0 432 1545
Size: 432 1113
PpuMI rG'GwC_Cy
Cuts at: 0 1342 1545
Size: 1342 203
PstI C_TGCA'G
Cuts at: 0 1523 1545
Size: 1523 22
PvuII CAG'CTG
Cuts at: 0 595 1545
Size: 595 950
SacI G_AGCT'C
Cuts at: 0 1455 1545
Size: 1455 90
SexAI A'CCwGG_T
Cuts at: 0 241 1545
Size: 241 1304
SfcI C'TryA_G
Cuts at: 0 563 1322 1519 1545
Size: 563 759 197 26
Fragments arranged by size:
759 563 197 26
SmlI C'TyrA_G
Cuts at: 0 807 1545
Size: 807 738
SpeI A'CTAG_T
Cuts at: 0 1041 1545
Size: 1041 504
SspI AAT'ATT
Cuts at: 0 1188 1292 1545
Size: 1188 104 253
Fragments arranged by size:
1188 253 104
TatI w'GTAC_w
Cuts at: 0 1326 1545
Size: 1326 219
Tth111I GACn'n_nGTC
Cuts at: 0 243 1545
Size: 243 1302
XbaI T'CTAG_A
Cuts at: 0 1353 1545
Size: 1353 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1215 1545
Size: 1215 330
XmnI GAAnn'nnTTC
Cuts at: 0 737 1545
Size: 737 808
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApoI AseI
BanII BbsI BclI BfrBI BmtI BpmI BpuEI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI CspCI DrdI EarI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MslI
MspA1I NdeI NheI NsiI NspI PciI PpuMI PstI
PvuII SacI SexAI SfcI SmlI SpeI SspI TatI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BtgI BtsI ClaI DraI DraIII EaeI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI StyI SwaI TaqII TstI XhoI XmaI ZraI