Restriction map for
pFN21AE6466
(Linear) (Six-base) MAPSORT of: KIEE6466.seq Check: 7507 from: 1 to: 1842
KIEE6466.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 392 1842
Size: 392 1450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1696 1842
Size: 1079 617 146
Fragments arranged by size:
1079 617 146
AflIII A'CryG_T
Cuts at: 0 1226 1842
Size: 1226 616
AhdI GACnn_n'nnGTC
Cuts at: 0 1685 1842
Size: 1685 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1396 1842
Size: 1396 446
AlwNI CAG_nnn'CTG
Cuts at: 0 1078 1842
Size: 1078 764
ApaI G_GGCC'C
Cuts at: 0 1793 1842
Size: 1793 49
ApoI r'AATT_y
Cuts at: 0 546 568 1842
Size: 546 22 1274
Fragments arranged by size:
1274 546 22
AvaI C'yCGr_G
Cuts at: 0 55 600 1682 1842
Size: 55 545 1082 160
Fragments arranged by size:
1082 545 160 55
AvrII C'CTAG_G
Cuts at: 0 1170 1842
Size: 1170 672
BanI G'GyrC_C
Cuts at: 0 862 899 1047 1842
Size: 862 37 148 795
Fragments arranged by size:
862 795 148 37
BanII G_rGCy'C
Cuts at: 0 420 622 1268 1793 1842
Size: 420 202 646 525 49
Fragments arranged by size:
646 525 420 202 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 1002 1684 1842
Size: 89 913 682 158
Fragments arranged by size:
913 682 158 89
BciVI GTATCCnnnnn_n'
Cuts at: 0 1597 1842
Size: 1597 245
BclI T'GATC_A
Cuts at: 0 761 1842
Size: 761 1081
BglII A'GATC_T
Cuts at: 0 329 869 1493 1842
Size: 329 540 624 349
Fragments arranged by size:
624 540 349 329
Bme1580I G_kGCm'C
Cuts at: 0 865 1052 1793 1842
Size: 865 187 741 49
Fragments arranged by size:
865 741 187 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 295 342 1713 1842
Size: 198 97 47 1371 129
Fragments arranged by size:
1371 198 129 97 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1552 1569 1842
Size: 817 735 17 273
Fragments arranged by size:
817 735 273 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 48 1825 1842
Size: 48 1777 17
Fragments arranged by size:
1777 48 17
BsaBI GATnn'nnATC
Cuts at: 0 1336 1842
Size: 1336 506
BsaWI w'CCGG_w
Cuts at: 0 1244 1842
Size: 1244 598
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 604 634 1842
Size: 268 30 306 30 1208
Fragments arranged by size:
1208 306 268 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1258 1842
Size: 73 1185 584
Fragments arranged by size:
1185 584 73
BseYI C'CCAG_C
Cuts at: 0 165 629 997 1130 1418 1842
Size: 165 464 368 133 288 424
Fragments arranged by size:
464 424 368 288 165 133
BsiHKAI G_wGCw'C
Cuts at: 0 1268 1842
Size: 1268 574
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 1842
Size: 573 1269
Bsp1286I G_dGCh'C
Cuts at: 0 420 622 865 1052 1268 1793 1842
Size: 420 202 243 187 216 525 49
Fragments arranged by size:
525 420 243 216 202 187 49
BspEI T'CCGG_A
Cuts at: 0 1244 1842
Size: 1244 598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 475 1842
Size: 475 1367
BsrBI CCG'CTC
Cuts at: 0 1253 1842
Size: 1253 589
BsrDI GCAATG_nn'
Cuts at: 0 177 492 1842
Size: 177 315 1350
Fragments arranged by size:
1350 315 177
BsrFI r'CCGG_y
Cuts at: 0 523 1472 1842
Size: 523 949 370
Fragments arranged by size:
949 523 370
BsrGI T'GTAC_A
Cuts at: 0 1334 1842
Size: 1334 508
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1047 1842
Size: 1047 795
BstEII G'GTnAC_C
Cuts at: 0 1317 1842
Size: 1317 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 352 993 1842
Size: 126 226 641 849
Fragments arranged by size:
849 641 226 126
BstYI r'GATC_y
Cuts at: 0 329 869 1493 1842
Size: 329 540 624 349
Fragments arranged by size:
624 540 349 329
Bsu36I CC'TnA_GG
Cuts at: 0 842 1842
Size: 842 1000
BtgI C'CryG_G
Cuts at: 0 1477 1637 1842
Size: 1477 160 205
Fragments arranged by size:
1477 205 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 942 1077 1266 1842
Size: 942 135 189 576
Fragments arranged by size:
942 576 189 135
BtsI GCAGTG_nn'
Cuts at: 0 23 1776 1842
Size: 23 1753 66
Fragments arranged by size:
1753 66 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 972 1007 1842
Size: 972 35 835
Fragments arranged by size:
972 835 35
EaeI y'GGCC_r
Cuts at: 0 146 1064 1474 1529 1776 1842
Size: 146 918 410 55 247 66
Fragments arranged by size:
918 410 247 146 66 55
EarI CTCTTCn'nnn_
Cuts at: 0 346 427 1243 1510 1546 1660 1842
Size: 346 81 816 267 36 114 182
Fragments arranged by size:
816 346 267 182 114 81 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 1447 1842
Size: 817 630 395
Fragments arranged by size:
817 630 395
EcoICRI GAG'CTC
Cuts at: 0 1266 1842
Size: 1266 576
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 295 342 1079 1696 1713 1842
Size: 198 97 47 737 617 17 129
Fragments arranged by size:
737 617 198 129 97 47 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 1842
Size: 343 1499
EcoO109I rG'GnC_Cy
Cuts at: 0 51 837 1075 1687 1790 1842
Size: 51 786 238 612 103 52
Fragments arranged by size:
786 612 238 103 52 51
EcoRI G'AATT_C
Cuts at: 0 568 1842
Size: 568 1274
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 1671 1703 1842
Size: 604 32 1035 32 139
Fragments arranged by size:
1035 604 139 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1842
Size: 53 1789
MscI TGG'CCA
Cuts at: 0 1531 1842
Size: 1531 311
MslI CAynn'nnrTG
Cuts at: 0 262 1225 1842
Size: 262 963 617
Fragments arranged by size:
963 617 262
MspA1I CmG'CkG
Cuts at: 0 1134 1347 1842
Size: 1134 213 495
Fragments arranged by size:
1134 495 213
NaeI GCC'GGC
Cuts at: 0 525 1842
Size: 525 1317
NcoI C'CATG_G
Cuts at: 0 1477 1637 1842
Size: 1477 160 205
Fragments arranged by size:
1477 205 160
NdeI CA'TA_TG
Cuts at: 0 1375 1842
Size: 1375 467
NgoMIV G'CCGG_C
Cuts at: 0 523 1842
Size: 523 1319
NspI r_CATG'y
Cuts at: 0 1230 1842
Size: 1230 612
PasI CC'CwG_GG
Cuts at: 0 912 1598 1842
Size: 912 686 244
Fragments arranged by size:
912 686 244
PciI A'CATG_T
Cuts at: 0 1226 1842
Size: 1226 616
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 1616 1842
Size: 615 1001 226
Fragments arranged by size:
1001 615 226
PfoI T'CCnGG_A
Cuts at: 0 272 780 1842
Size: 272 508 1062
Fragments arranged by size:
1062 508 272
PpuMI rG'GwC_Cy
Cuts at: 0 51 837 1075 1687 1842
Size: 51 786 238 612 155
Fragments arranged by size:
786 612 238 155 51
PshAI GACnn'nnGTC
Cuts at: 0 1730 1842
Size: 1730 112
PspOMI G'GGCC_C
Cuts at: 0 1789 1842
Size: 1789 53
PvuII CAG'CTG
Cuts at: 0 1134 1347 1842
Size: 1134 213 495
Fragments arranged by size:
1134 495 213
RsrII CG'GwC_CG
Cuts at: 0 1434 1842
Size: 1434 408
SacI G_AGCT'C
Cuts at: 0 1268 1842
Size: 1268 574
SanDI GG'GwC_CC
Cuts at: 0 51 1842
Size: 51 1791
SapI GCTCTTCn'nnn_
Cuts at: 0 1243 1510 1842
Size: 1243 267 332
Fragments arranged by size:
1243 332 267
SexAI A'CCwGG_T
Cuts at: 0 92 1842
Size: 92 1750
SmlI C'TyrA_G
Cuts at: 0 832 1548 1567 1842
Size: 832 716 19 275
Fragments arranged by size:
832 716 275 19
StyI C'CwwG_G
Cuts at: 0 303 1103 1170 1477 1637 1842
Size: 303 800 67 307 160 205
Fragments arranged by size:
800 307 303 205 160 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 880 1410 1842
Size: 880 530 432
Fragments arranged by size:
880 530 432
TatI w'GTAC_w
Cuts at: 0 1334 1556 1571 1842
Size: 1334 222 15 271
Fragments arranged by size:
1334 271 222 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 990 1059 1842
Size: 708 282 69 783
Fragments arranged by size:
783 708 282 69
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BciVI BclI BglII
Bme1580I BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PpuMI PshAI PspOMI PvuII RsrII SacI SanDI
SapI SexAI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaHI BsgI BsiEI BsiWI BsmI
BspHI BssHII BssSI BstBI BstZ17I ClaI DraI DraIII
DrdI EagI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI SacII SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI