Restriction map for
pFN21AE6464
(Linear) (Six-base) MAPSORT of: KIEE6464.seq Check: 9892 from: 1 to: 1623
KIEE6464.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 528 1623
Size: 528 1095
Acc65I G'GTAC_C
Cuts at: 0 475 1623
Size: 475 1148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 756 1623
Size: 648 108 867
Fragments arranged by size:
867 648 108
AflIII A'CryG_T
Cuts at: 0 365 497 1623
Size: 365 132 1126
Fragments arranged by size:
1126 365 132
AleI CACnn'nnGTG
Cuts at: 0 1239 1623
Size: 1239 384
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 1257 1623
Size: 265 992 366
Fragments arranged by size:
992 366 265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 1623
Size: 127 32 1464
Fragments arranged by size:
1464 127 32
ApaI G_GGCC'C
Cuts at: 0 631 1193 1623
Size: 631 562 430
Fragments arranged by size:
631 562 430
AvaI C'yCGr_G
Cuts at: 0 235 630 1205 1623
Size: 235 395 575 418
Fragments arranged by size:
575 418 395 235
BamHI G'GATC_C
Cuts at: 0 796 1623
Size: 796 827
BanI G'GyrC_C
Cuts at: 0 91 475 1411 1623
Size: 91 384 936 212
Fragments arranged by size:
936 384 212 91
BanII G_rGCy'C
Cuts at: 0 174 631 894 1177 1193 1623
Size: 174 457 263 283 16 430
Fragments arranged by size:
457 430 283 263 174 16
BbvCI CC'TCA_GC
Cuts at: 0 538 801 1149 1303 1623
Size: 538 263 348 154 320
Fragments arranged by size:
538 348 320 263 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 927 961 1594 1623
Size: 927 34 633 29
Fragments arranged by size:
927 633 34 29
BglI GCCn_nnn'nGGC
Cuts at: 0 811 919 1623
Size: 811 108 704
Fragments arranged by size:
811 704 108
BglII A'GATC_T
Cuts at: 0 1135 1310 1607 1623
Size: 1135 175 297 16
Fragments arranged by size:
1135 297 175 16
BlpI GC'TnA_GC
Cuts at: 0 124 1623
Size: 124 1499
Bme1580I G_kGCm'C
Cuts at: 0 96 631 672 915 965 998 1193 1623
Size: 96 535 41 243 50 33 195 430
Fragments arranged by size:
535 430 243 195 96 50 41 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1374 1623
Size: 1374 249
BmtI G_CTAG'C
Cuts at: 0 904 1623
Size: 904 719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1046 1160 1623
Size: 586 460 114 463
Fragments arranged by size:
586 463 460 114
Bpu10I CC'TnA_GC
Cuts at: 0 538 801 1149 1171 1303 1555 1623
Size: 538 263 348 22 132 252 68
Fragments arranged by size:
538 348 263 252 132 68 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 1136 1623
Size: 1136 487
BsaAI yAC'GTr
Cuts at: 0 366 1623
Size: 366 1257
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 389 419 1623
Size: 127 30 232 30 1204
Fragments arranged by size:
1204 232 127 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 333 535 1041 1148 1623
Size: 166 167 202 506 107 475
Fragments arranged by size:
506 475 202 167 166 107
BseYI C'CCAG_C
Cuts at: 0 48 506 624 1623
Size: 48 458 118 999
Fragments arranged by size:
999 458 118 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 899 1623
Size: 500 399 724
Fragments arranged by size:
724 500 399
BsiHKAI G_wGCw'C
Cuts at: 0 719 1177 1623
Size: 719 458 446
Fragments arranged by size:
719 458 446
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1536 1623
Size: 1536 87
Bsp1286I G_dGCh'C
Cuts at: 0 96 174 631 672 719 894 915 965
Size: 96 78 457 41 47 175 21 50
Cuts at: 965 998 1177 1193 1623
Size: 33 179 16 430
Fragments arranged by size:
457 430 179 175 96 78 50 47
41 33 21 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 528 1623
Size: 528 1095
BsrBI CCG'CTC
Cuts at: 0 1204 1623
Size: 1204 419
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1218 1623
Size: 1218 405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 867 1292 1623
Size: 867 425 331
Fragments arranged by size:
867 425 331
BstYI r'GATC_y
Cuts at: 0 796 1135 1310 1607 1623
Size: 796 339 175 297 16
Fragments arranged by size:
796 339 297 175 16
BtgI C'CryG_G
Cuts at: 0 347 385 431 640 789 860 965 1623
Size: 347 38 46 209 149 71 105 658
Fragments arranged by size:
658 347 209 149 105 71 46 38
BtsI GCAGTG_nn'
Cuts at: 0 103 512 839 1106 1216 1234 1623
Size: 103 409 327 267 110 18 389
Fragments arranged by size:
409 389 327 267 110 103 18
EaeI y'GGCC_r
Cuts at: 0 1370 1563 1623
Size: 1370 193 60
Fragments arranged by size:
1370 193 60
EarI CTCTTCn'nnn_
Cuts at: 0 553 667 724 1329 1384 1534 1623
Size: 553 114 57 605 55 150 89
Fragments arranged by size:
605 553 150 114 89 57 55
EcoICRI GAG'CTC
Cuts at: 0 1175 1623
Size: 1175 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 648 756 1046 1160 1623
Size: 586 62 108 290 114 463
Fragments arranged by size:
586 463 290 114 108 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 415 1042 1623
Size: 415 627 581
Fragments arranged by size:
627 581 415
EcoO109I rG'GnC_Cy
Cuts at: 0 588 615 1190 1623
Size: 588 27 575 433
Fragments arranged by size:
588 575 433 27
KpnI G_GTAC'C
Cuts at: 0 479 1623
Size: 479 1144
MscI TGG'CCA
Cuts at: 0 1565 1623
Size: 1565 58
MslI CAynn'nnrTG
Cuts at: 0 430 1239 1623
Size: 430 809 384
Fragments arranged by size:
809 430 384
MspA1I CmG'CkG
Cuts at: 0 393 1456 1623
Size: 393 1063 167
Fragments arranged by size:
1063 393 167
NcoI C'CATG_G
Cuts at: 0 347 789 860 965 1623
Size: 347 442 71 105 658
Fragments arranged by size:
658 442 347 105 71
NheI G'CTAG_C
Cuts at: 0 900 1623
Size: 900 723
NspI r_CATG'y
Cuts at: 0 501 684 1216 1623
Size: 501 183 532 407
Fragments arranged by size:
532 501 407 183
PasI CC'CwG_GG
Cuts at: 0 273 482 1186 1623
Size: 273 209 704 437
Fragments arranged by size:
704 437 273 209
PciI A'CATG_T
Cuts at: 0 497 1623
Size: 497 1126
PflMI CCAn_nnn'nTGG
Cuts at: 0 1512 1623
Size: 1512 111
PfoI T'CCnGG_A
Cuts at: 0 1426 1623
Size: 1426 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 1623
Size: 127 32 1464
Fragments arranged by size:
1464 127 32
PpuMI rG'GwC_Cy
Cuts at: 0 588 1623
Size: 588 1035
PshAI GACnn'nnGTC
Cuts at: 0 1456 1623
Size: 1456 167
PspOMI G'GGCC_C
Cuts at: 0 627 1189 1623
Size: 627 562 434
Fragments arranged by size:
627 562 434
PstI C_TGCA'G
Cuts at: 0 110 1046 1623
Size: 110 936 577
Fragments arranged by size:
936 577 110
SacI G_AGCT'C
Cuts at: 0 1177 1623
Size: 1177 446
SapI GCTCTTCn'nnn_
Cuts at: 0 724 1329 1623
Size: 724 605 294
Fragments arranged by size:
724 605 294
SfcI C'TryA_G
Cuts at: 0 106 1042 1623
Size: 106 936 581
Fragments arranged by size:
936 581 106
SmaI CCC'GGG
Cuts at: 0 632 1623
Size: 632 991
SphI G_CATG'C
Cuts at: 0 1216 1623
Size: 1216 407
StuI AGG'CCT
Cuts at: 0 944 1019 1623
Size: 944 75 604
Fragments arranged by size:
944 604 75
StyI C'CwwG_G
Cuts at: 0 347 789 860 965 1069 1623
Size: 347 442 71 105 104 554
Fragments arranged by size:
554 442 347 105 104 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 1623
Size: 40 1583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 509 541 1623
Size: 389 32 88 32 1082
Fragments arranged by size:
1082 389 88 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 867 1135 1623
Size: 702 165 268 488
Fragments arranged by size:
702 488 268 165
XmaI C'CCGG_G
Cuts at: 0 630 1623
Size: 630 993
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AloI ApaI
AvaI BamHI BanI BanII BbvCI BcgI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BstAPI BstXI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I KpnI MscI MslI MspA1I
NcoI NheI NspI PasI PciI PflMI PfoI PpiI
PpuMI PshAI PspOMI PstI SacI SapI SfcI SmaI
SphI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BciVI BclI BfrBI BmgBI BplI BpuEI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PmeI
PmlI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI