(Linear) (Six-base) MAPSORT of: KIEE6463.seq  Check: 8154  from: 1  to: 1500



KIEE6463.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887     917    1500 
   Size:        887      30     583

  Fragments arranged by size: 

                 887     583      30
 
AflIII A'CryG_T

Cuts at:      0     233    1500 
   Size:        233    1267
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361     524     738    1288    1500 
   Size:        361     163     214     550     212

  Fragments arranged by size: 

                 550     361     214     212     163
 
ApoI r'AATT_y

Cuts at:      0     914    1091    1500 
   Size:        914     177     409

  Fragments arranged by size: 

                 914     409     177
 
AvrII C'CTAG_G

Cuts at:      0     381    1500 
   Size:        381    1119
 
BamHI G'GATC_C

Cuts at:      0    1213    1500 
   Size:       1213     287
 
BanII G_rGCy'C

Cuts at:      0      39    1500 
   Size:         39    1461
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1085    1500 
   Size:       1085     415
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      53    1500 
   Size:         53    1447
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     495    1500 
   Size:        495    1005
 
BseYI C'CCAG_C

Cuts at:      0     746    1500 
   Size:        746     754
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     548     566    1500 
   Size:         50     498      18     934

  Fragments arranged by size: 

                 934     498      50      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     109    1500 
   Size:        109    1391
 
Bsp1286I G_dGCh'C

Cuts at:      0      39    1500 
   Size:         39    1461
 
BssSI C'ACGA_G

Cuts at:      0     177    1500 
   Size:        177    1323
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     689    1500 
   Size:        689     811
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     714    1500 
   Size:        714     786
 
BstYI r'GATC_y

Cuts at:      0     430     870    1213    1500 
   Size:        430     440     343     287

  Fragments arranged by size: 

                 440     430     343     287
 
BtgI C'CryG_G

Cuts at:      0    1249    1500 
   Size:       1249     251
 
BtsI GCAGTG_nn'

Cuts at:      0      62    1500 
   Size:         62    1438
 
DraIII CAC_nnn'GTG

Cuts at:      0     880    1500 
   Size:        880     620
 
EaeI y'GGCC_r

Cuts at:      0     242    1500 
   Size:        242    1258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     887     917    1500 
   Size:        887      30     583

  Fragments arranged by size: 

                 887     583      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      80     856    1500 
   Size:         80     776     644

  Fragments arranged by size: 

                 776     644      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86    1500 
   Size:         86    1414
 
EcoRV GAT'ATC

Cuts at:      0    1034    1500 
   Size:       1034     466
 
HincII GTy'rAC

Cuts at:      0     763    1500 
   Size:        763     737
 
MfeI C'AATT_G

Cuts at:      0    1151    1500 
   Size:       1151     349
 
MscI TGG'CCA

Cuts at:      0     244    1500 
   Size:        244    1256
 
MslI CAynn'nnrTG

Cuts at:      0     238     501    1373    1500 
   Size:        238     263     872     127

  Fragments arranged by size: 

                 872     263     238     127
 
MspA1I CmG'CkG

Cuts at:      0     346    1500 
   Size:        346    1154
 
NcoI C'CATG_G

Cuts at:      0    1249    1500 
   Size:       1249     251
 
NspI r_CATG'y

Cuts at:      0     237     894    1500 
   Size:        237     657     606

  Fragments arranged by size: 

                 657     606     237
 
PciI A'CATG_T

Cuts at:      0     233    1500 
   Size:        233    1267
 
PpuMI rG'GwC_Cy

Cuts at:      0      86    1500 
   Size:         86    1414
 
PsiI TTA'TAA

Cuts at:      0     443     673    1273    1500 
   Size:        443     230     600     227

  Fragments arranged by size: 

                 600     443     230     227
 
PstI C_TGCA'G

Cuts at:      0    1293    1500 
   Size:       1293     207
 
SfcI C'TryA_G

Cuts at:      0    1289    1500 
   Size:       1289     211
 
SspI AAT'ATT

Cuts at:      0     803    1500 
   Size:        803     697
 
StyI C'CwwG_G

Cuts at:      0     109     381     426     450    1239    1249    1500 
   Size:        109     272      45      24     789      10     251

  Fragments arranged by size: 

                 789     272     251     109      45      24      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145    1500 
   Size:        145    1355
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1205    1237    1500 
   Size:       1205      32     263

  Fragments arranged by size: 

                1205     263      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     743    1500 
   Size:        743     757
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    AvrII    BamHI    BanII     BmrI
     BplI     BsaI    BseYI     BsgI    BsmBI Bsp1286I    BssSI   BstAPI
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI  Eco57MI    EcoNI
 EcoO109I    EcoRV   HincII     MfeI     MscI     MslI   MspA1I     NcoI
     NspI     PciI    PpuMI     PsiI     PstI     SfcI     SspI     StyI
    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI