Restriction map for
pFN21AE6462
(Linear) (Six-base) MAPSORT of: KIEE6462.seq Check: 7840 from: 1 to: 1482
KIEE6462.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 586 1482
Size: 69 517 896
Fragments arranged by size:
896 517 69
AflIII A'CryG_T
Cuts at: 0 173 1482
Size: 173 1309
AhdI GACnn_n'nnGTC
Cuts at: 0 990 1482
Size: 990 492
ApoI r'AATT_y
Cuts at: 0 327 1482
Size: 327 1155
AseI AT'TA_AT
Cuts at: 0 757 761 1423 1482
Size: 757 4 662 59
Fragments arranged by size:
757 662 59 4
AvaI C'yCGr_G
Cuts at: 0 708 1482
Size: 708 774
BanII G_rGCy'C
Cuts at: 0 1244 1482
Size: 1244 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 785 1482
Size: 746 39 697
Fragments arranged by size:
746 697 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 255 1482
Size: 255 1227
BglII A'GATC_T
Cuts at: 0 197 1482
Size: 197 1285
BmgBI CAC'GTC
Cuts at: 0 176 1482
Size: 176 1306
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 218 1482
Size: 36 182 1264
Fragments arranged by size:
1264 182 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1481 1482
Size: 1481 1
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 564 890 1482
Size: 222 342 326 592
Fragments arranged by size:
592 342 326 222
Bpu10I CC'TnA_GC
Cuts at: 0 1453 1482
Size: 1453 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 544 709 1482
Size: 35 509 165 773
Fragments arranged by size:
773 509 165 35
BsaBI GATnn'nnATC
Cuts at: 0 664 1482
Size: 664 818
BsaHI Gr'CG_yC
Cuts at: 0 637 1482
Size: 637 845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 537 567 1301 1331 1482
Size: 537 30 734 30 151
Fragments arranged by size:
734 537 151 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 539 1482
Size: 539 943
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 664 1482
Size: 248 416 818
Fragments arranged by size:
818 416 248
BsiHKAI G_wGCw'C
Cuts at: 0 361 1244 1482
Size: 361 883 238
Fragments arranged by size:
883 361 238
Bsp1286I G_dGCh'C
Cuts at: 0 361 1244 1482
Size: 361 883 238
Fragments arranged by size:
883 361 238
BspHI T'CATG_A
Cuts at: 0 1388 1482
Size: 1388 94
BsrDI GCAATG_nn'
Cuts at: 0 132 1482
Size: 132 1350
BsrFI r'CCGG_y
Cuts at: 0 1071 1482
Size: 1071 411
BsrGI T'GTAC_A
Cuts at: 0 1399 1482
Size: 1399 83
BssSI C'ACGA_G
Cuts at: 0 1447 1482
Size: 1447 35
BstYI r'GATC_y
Cuts at: 0 197 1482
Size: 197 1285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 348 1482
Size: 348 1134
BtsI GCAGTG_nn'
Cuts at: 0 157 1263 1322 1482
Size: 157 1106 59 160
Fragments arranged by size:
1106 160 157 59
EaeI y'GGCC_r
Cuts at: 0 7 1268 1482
Size: 7 1261 214
Fragments arranged by size:
1261 214 7
EarI CTCTTCn'nnn_
Cuts at: 0 280 366 1057 1482
Size: 280 86 691 425
Fragments arranged by size:
691 425 280 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 991 1482
Size: 991 491
EcoICRI GAG'CTC
Cuts at: 0 1242 1482
Size: 1242 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 222 564 586 890 1482
Size: 69 153 342 22 304 592
Fragments arranged by size:
592 342 304 153 69 22
EcoO109I rG'GnC_Cy
Cuts at: 0 1106 1482
Size: 1106 376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 259 1482
Size: 259 1223
HaeII r_GCGC'y
Cuts at: 0 1072 1482
Size: 1072 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1482
Size: 976 32 474
Fragments arranged by size:
976 474 32
HindIII A'AGCT_T
Cuts at: 0 843 1262 1482
Size: 843 419 220
Fragments arranged by size:
843 419 220
MfeI C'AATT_G
Cuts at: 0 1403 1482
Size: 1403 79
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 1482
Size: 990 492
MscI TGG'CCA
Cuts at: 0 9 1270 1482
Size: 9 1261 212
Fragments arranged by size:
1261 212 9
MslI CAynn'nnrTG
Cuts at: 0 56 1387 1482
Size: 56 1331 95
Fragments arranged by size:
1331 95 56
NspI r_CATG'y
Cuts at: 0 357 860 1482
Size: 357 503 622
Fragments arranged by size:
622 503 357
PacI TTA_AT'TAA
Cuts at: 0 761 1482
Size: 761 721
PflMI CCAn_nnn'nTGG
Cuts at: 0 473 1482
Size: 473 1009
PfoI T'CCnGG_A
Cuts at: 0 867 1343 1482
Size: 867 476 139
Fragments arranged by size:
867 476 139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1058 1090 1482
Size: 1058 32 392
Fragments arranged by size:
1058 392 32
PpuMI rG'GwC_Cy
Cuts at: 0 1106 1482
Size: 1106 376
PshAI GACnn'nnGTC
Cuts at: 0 1229 1482
Size: 1229 253
PstI C_TGCA'G
Cuts at: 0 464 1317 1482
Size: 464 853 165
Fragments arranged by size:
853 464 165
SacI G_AGCT'C
Cuts at: 0 1244 1482
Size: 1244 238
SanDI GG'GwC_CC
Cuts at: 0 1106 1482
Size: 1106 376
SapI GCTCTTCn'nnn_
Cuts at: 0 366 1482
Size: 366 1116
SfcI C'TryA_G
Cuts at: 0 460 1057 1313 1482
Size: 460 597 256 169
Fragments arranged by size:
597 460 256 169
SspI AAT'ATT
Cuts at: 0 1439 1482
Size: 1439 43
TatI w'GTAC_w
Cuts at: 0 428 1053 1399 1482
Size: 428 625 346 83
Fragments arranged by size:
625 428 346 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 147 179 537 569 668 700 1008 1040
Size: 147 32 358 32 99 32 308 32
Cuts at: 1040 1301 1333 1482
Size: 261 32 149
Fragments arranged by size:
358 308 261 149 147 99 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 636 1482
Size: 636 846
Enzymes that do cut:
AcuI AflIII AhdI ApoI AseI AvaI BanII BbsI
BciVI BglII BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI
BsrDI BsrFI BsrGI BssSI BstYI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
HindIII MfeI MmeI MscI MslI NspI PacI PflMI
PfoI PpiI PpuMI PshAI PstI SacI SanDI SapI
SfcI SspI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BfrBI BglI BlpI Bme1580I BmtI BpuEI BsaAI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PasI PciI PmeI PmlI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII XbaI XcmI XhoI XmaI XmnI ZraI