Restriction map for
pFN21AE6461
(Linear) (Six-base) MAPSORT of: KIEE6461.seq Check: 8757 from: 1 to: 1425
KIEE6461.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 25 44 945 1425
Size: 25 19 901 480
Fragments arranged by size:
901 480 25 19
AflIII A'CryG_T
Cuts at: 0 1115 1304 1425
Size: 1115 189 121
Fragments arranged by size:
1115 189 121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 1425
Size: 352 1073
AlwNI CAG_nnn'CTG
Cuts at: 0 615 1425
Size: 615 810
AvaI C'yCGr_G
Cuts at: 0 7 51 185 361 1130 1425
Size: 7 44 134 176 769 295
Fragments arranged by size:
769 295 176 134 44 7
BamHI G'GATC_C
Cuts at: 0 768 1425
Size: 768 657
BanI G'GyrC_C
Cuts at: 0 803 1425
Size: 803 622
BanII G_rGCy'C
Cuts at: 0 7 71 195 490 719 1086 1204 1318
Size: 7 64 124 295 229 367 118 114
Cuts at: 1318 1425
Size: 107
Fragments arranged by size:
367 295 229 124 118 114 107 64
7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1011 1425
Size: 1011 414
BbvCI CC'TCA_GC
Cuts at: 0 925 1425
Size: 925 500
BciVI GTATCCnnnnn_n'
Cuts at: 0 723 1425
Size: 723 702
BclI T'GATC_A
Cuts at: 0 774 1049 1425
Size: 774 275 376
Fragments arranged by size:
774 376 275
BglII A'GATC_T
Cuts at: 0 317 1283 1425
Size: 317 966 142
Fragments arranged by size:
966 317 142
BlpI GC'TnA_GC
Cuts at: 0 295 1425
Size: 295 1130
Bme1580I G_kGCm'C
Cuts at: 0 602 806 898 1425
Size: 602 204 92 527
Fragments arranged by size:
602 527 204 92
BmgBI CAC'GTC
Cuts at: 0 906 1425
Size: 906 519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 950 1105 1425
Size: 950 155 320
Fragments arranged by size:
950 320 155
Bpu10I CC'TnA_GC
Cuts at: 0 925 1425
Size: 925 500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1425
Size: 358 1067
BsaI GGTCTCn'nnnn_
Cuts at: 0 1071 1425
Size: 1071 354
BsaBI GATnn'nnATC
Cuts at: 0 322 773 1425
Size: 322 451 652
Fragments arranged by size:
652 451 322
BsaHI Gr'CG_yC
Cuts at: 0 136 1425
Size: 136 1289
BsaWI w'CCGG_w
Cuts at: 0 148 1425
Size: 148 1277
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1425
Size: 391 30 1004
Fragments arranged by size:
1004 391 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 147 480 1078 1194 1323 1425
Size: 112 35 333 598 116 129 102
Fragments arranged by size:
598 333 129 116 112 102 35
BseYI C'CCAG_C
Cuts at: 0 285 339 474 483 537 595 706 1425
Size: 285 54 135 9 54 58 111 719
Fragments arranged by size:
719 285 135 111 58 54 54 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1425
Size: 973 452
BsiEI CG_ry'CG
Cuts at: 0 303 1425
Size: 303 1122
BsiHKAI G_wGCw'C
Cuts at: 0 719 1425
Size: 719 706
Bsp1286I G_dGCh'C
Cuts at: 0 7 71 195 490 602 719 806 898
Size: 7 64 124 295 112 117 87 92
Cuts at: 898 1086 1204 1318 1425
Size: 188 118 114 107
Fragments arranged by size:
295 188 124 118 117 114 112 107
92 87 64 7
BspHI T'CATG_A
Cuts at: 0 1052 1425
Size: 1052 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 25 44 945 1425
Size: 25 19 901 480
Fragments arranged by size:
901 480 25 19
BsrBI CCG'CTC
Cuts at: 0 506 1425
Size: 506 919
BsrDI GCAATG_nn'
Cuts at: 0 845 1425
Size: 845 580
BsrFI r'CCGG_y
Cuts at: 0 1252 1425
Size: 1252 173
BsrGI T'GTAC_A
Cuts at: 0 1274 1425
Size: 1274 151
BssSI C'ACGA_G
Cuts at: 0 124 1425
Size: 124 1301
BstEII G'GTnAC_C
Cuts at: 0 12 1425
Size: 12 1413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1005 1425
Size: 1005 420
BstYI r'GATC_y
Cuts at: 0 317 768 1283 1425
Size: 317 451 515 142
Fragments arranged by size:
515 451 317 142
BtgI C'CryG_G
Cuts at: 0 22 725 1298 1425
Size: 22 703 573 127
Fragments arranged by size:
703 573 127 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 164 1425
Size: 164 1261
BtsI GCAGTG_nn'
Cuts at: 0 803 1425
Size: 803 622
EaeI y'GGCC_r
Cuts at: 0 391 698 1352 1425
Size: 391 307 654 73
Fragments arranged by size:
654 391 307 73
EarI CTCTTCn'nnn_
Cuts at: 0 586 1425
Size: 586 839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 264 438 1425
Size: 264 174 987
Fragments arranged by size:
987 264 174
EcoICRI GAG'CTC
Cuts at: 0 717 1425
Size: 717 708
EcoO109I rG'GnC_Cy
Cuts at: 0 56 612 684 1425
Size: 56 556 72 741
Fragments arranged by size:
741 556 72 56
HaeII r_GCGC'y
Cuts at: 0 424 475 1425
Size: 424 51 950
Fragments arranged by size:
950 424 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 721 753 1425
Size: 721 32 672
Fragments arranged by size:
721 672 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 761 922 1425
Size: 185 576 161 503
Fragments arranged by size:
576 503 185 161
MscI TGG'CCA
Cuts at: 0 393 700 1354 1425
Size: 393 307 654 71
Fragments arranged by size:
654 393 307 71
MslI CAynn'nnrTG
Cuts at: 0 328 1045 1051 1303 1309 1425
Size: 328 717 6 252 6 116
Fragments arranged by size:
717 328 252 116 6 6
MspA1I CmG'CkG
Cuts at: 0 24 1028 1236 1425
Size: 24 1004 208 189
Fragments arranged by size:
1004 208 189 24
NcoI C'CATG_G
Cuts at: 0 1298 1425
Size: 1298 127
NdeI CA'TA_TG
Cuts at: 0 1404 1425
Size: 1404 21
NspI r_CATG'y
Cuts at: 0 333 1119 1308 1425
Size: 333 786 189 117
Fragments arranged by size:
786 333 189 117
PciI A'CATG_T
Cuts at: 0 1115 1304 1425
Size: 1115 189 121
Fragments arranged by size:
1115 189 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 817 1425
Size: 817 608
PfoI T'CCnGG_A
Cuts at: 0 816 1425
Size: 816 609
PpuMI rG'GwC_Cy
Cuts at: 0 56 684 1425
Size: 56 628 741
Fragments arranged by size:
741 628 56
PspXI vC'TCGA_Gb
Cuts at: 0 1130 1425
Size: 1130 295
PstI C_TGCA'G
Cuts at: 0 634 978 1235 1425
Size: 634 344 257 190
Fragments arranged by size:
634 344 257 190
PvuII CAG'CTG
Cuts at: 0 1236 1425
Size: 1236 189
SacI G_AGCT'C
Cuts at: 0 719 1425
Size: 719 706
SacII CC_GC'GG
Cuts at: 0 25 1425
Size: 25 1400
SfcI C'TryA_G
Cuts at: 0 630 663 974 1231 1425
Size: 630 33 311 257 194
Fragments arranged by size:
630 311 257 194 33
SmaI CCC'GGG
Cuts at: 0 187 1425
Size: 187 1238
SmlI C'TyrA_G
Cuts at: 0 373 1130 1425
Size: 373 757 295
Fragments arranged by size:
757 373 295
StyI C'CwwG_G
Cuts at: 0 1241 1298 1425
Size: 1241 57 127
Fragments arranged by size:
1241 127 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 853 997 1425
Size: 853 144 428
Fragments arranged by size:
853 428 144
TatI w'GTAC_w
Cuts at: 0 1274 1425
Size: 1274 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 1425
Size: 753 32 640
Fragments arranged by size:
753 640 32
XbaI T'CTAG_A
Cuts at: 0 195 1425
Size: 195 1230
XhoI C'TCGA_G
Cuts at: 0 1130 1425
Size: 1130 295
XmaI C'CCGG_G
Cuts at: 0 185 1425
Size: 185 1240
Enzymes that do cut:
AarI AflIII AlfI AlwNI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI
BtgI BtgZI BtsI EaeI EarI EciI EcoICRI EcoO109I
HaeII Hin4I MmeI MscI MslI MspA1I NcoI NdeI
NspI PciI PflMI PfoI PpuMI PspXI PstI PvuII
SacI SacII SfcI SmaI SmlI StyI TaqII TatI
TstI XbaI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BfrBI BglI BmrI
BmtI BpmI BsaAI BsiWI BsmI BsmBI BspEI BssHII
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XcmI XmnI ZraI