Restriction map for
pFN21AE6460
(Linear) (Six-base) MAPSORT of: KIEE6460.seq Check: 4629 from: 1 to: 1407
KIEE6460.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 26 262 1407
Size: 26 236 1145
Fragments arranged by size:
1145 236 26
Acc65I G'GTAC_C
Cuts at: 0 113 1407
Size: 113 1294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1407
Size: 350 1057
AfeI AGC'GCT
Cuts at: 0 1331 1407
Size: 1331 76
AleI CACnn'nnGTG
Cuts at: 0 1178 1407
Size: 1178 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 897 1407
Size: 897 510
AlwNI CAG_nnn'CTG
Cuts at: 0 740 1223 1407
Size: 740 483 184
Fragments arranged by size:
740 483 184
ApaI G_GGCC'C
Cuts at: 0 164 823 1407
Size: 164 659 584
Fragments arranged by size:
659 584 164
AvaI C'yCGr_G
Cuts at: 0 103 1407
Size: 103 1304
BanI G'GyrC_C
Cuts at: 0 113 1407
Size: 113 1294
BanII G_rGCy'C
Cuts at: 0 46 68 164 481 823 1153 1318 1407
Size: 46 22 96 317 342 330 165 89
Fragments arranged by size:
342 330 317 165 96 89 46 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 24 657 764 1407
Size: 24 633 107 643
Fragments arranged by size:
643 633 107 24
BbvCI CC'TCA_GC
Cuts at: 0 1018 1228 1270 1407
Size: 1018 210 42 137
Fragments arranged by size:
1018 210 137 42
BglI GCCn_nnn'nGGC
Cuts at: 0 463 827 1407
Size: 463 364 580
Fragments arranged by size:
580 463 364
Bme1580I G_kGCm'C
Cuts at: 0 164 218 823 1407
Size: 164 54 605 584
Fragments arranged by size:
605 584 164 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 1185 1407
Size: 1185 222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1351 1407
Size: 1351 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 706 1407
Size: 646 60 701
Fragments arranged by size:
701 646 60
Bpu10I CC'TnA_GC
Cuts at: 0 1018 1228 1270 1407
Size: 1018 210 42 137
Fragments arranged by size:
1018 210 137 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 490 1056 1079 1407
Size: 490 566 23 328
Fragments arranged by size:
566 490 328 23
BsaWI w'CCGG_w
Cuts at: 0 481 1407
Size: 481 926
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 1407
Size: 106 30 1271
Fragments arranged by size:
1271 106 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 754 880 1407
Size: 664 90 126 527
Fragments arranged by size:
664 527 126 90
BseYI C'CCAG_C
Cuts at: 0 196 234 740 1248 1311 1407
Size: 196 38 506 508 63 96
Fragments arranged by size:
508 506 196 96 63 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 982 1327 1378 1407
Size: 342 640 345 51 29
Fragments arranged by size:
640 345 342 51 29
Bsp1286I G_dGCh'C
Cuts at: 0 46 68 164 218 481 823 1153 1318
Size: 46 22 96 54 263 342 330 165
Cuts at: 1318 1407
Size: 89
Fragments arranged by size:
342 330 263 165 96 89 54 46
22
BspHI T'CATG_A
Cuts at: 0 386 686 1407
Size: 386 300 721
Fragments arranged by size:
721 386 300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 125 262 502 1120 1407
Size: 26 99 137 240 618 287
Fragments arranged by size:
618 287 240 137 99 26
BsrFI r'CCGG_y
Cuts at: 0 392 1407
Size: 392 1015
BssSI C'ACGA_G
Cuts at: 0 543 703 1203 1407
Size: 543 160 500 204
Fragments arranged by size:
543 500 204 160
BstEII G'GTnAC_C
Cuts at: 0 51 171 777 1407
Size: 51 120 606 630
Fragments arranged by size:
630 606 120 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 690 1180 1407
Size: 582 108 490 227
Fragments arranged by size:
582 490 227 108
BtgI C'CryG_G
Cuts at: 0 752 1136 1376 1407
Size: 752 384 240 31
Fragments arranged by size:
752 384 240 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 543 1407
Size: 300 243 864
Fragments arranged by size:
864 300 243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 405 440 1407
Size: 405 35 967
Fragments arranged by size:
967 405 35
EaeI y'GGCC_r
Cuts at: 0 149 303 1028 1133 1407
Size: 149 154 725 105 274
Fragments arranged by size:
725 274 154 149 105
EarI CTCTTCn'nnn_
Cuts at: 0 369 800 1372 1407
Size: 369 431 572 35
Fragments arranged by size:
572 431 369 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 579 1407
Size: 579 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 646 706 1407
Size: 350 296 60 701
Fragments arranged by size:
701 350 296 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1165 1407
Size: 1165 242
EcoO109I rG'GnC_Cy
Cuts at: 0 161 820 1092 1109 1407
Size: 161 659 272 17 298
Fragments arranged by size:
659 298 272 161 17
EcoRV GAT'ATC
Cuts at: 0 515 1407
Size: 515 892
HaeII r_GCGC'y
Cuts at: 0 1283 1333 1407
Size: 1283 50 74
Fragments arranged by size:
1283 74 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 1407
Size: 346 32 1029
Fragments arranged by size:
1029 346 32
HincII GTy'rAC
Cuts at: 0 730 930 1353 1407
Size: 730 200 423 54
Fragments arranged by size:
730 423 200 54
KpnI G_GTAC'C
Cuts at: 0 117 1407
Size: 117 1290
MscI TGG'CCA
Cuts at: 0 151 1030 1135 1407
Size: 151 879 105 272
Fragments arranged by size:
879 272 151 105
MslI CAynn'nnrTG
Cuts at: 0 688 1135 1141 1178 1407
Size: 688 447 6 37 229
Fragments arranged by size:
688 447 229 37 6
MspA1I CmG'CkG
Cuts at: 0 147 200 443 1220 1311 1378 1407
Size: 147 53 243 777 91 67 29
Fragments arranged by size:
777 243 147 91 67 53 29
NaeI GCC'GGC
Cuts at: 0 394 1407
Size: 394 1013
NcoI C'CATG_G
Cuts at: 0 1136 1407
Size: 1136 271
NgoMIV G'CCGG_C
Cuts at: 0 392 1407
Size: 392 1015
NspI r_CATG'y
Cuts at: 0 951 1407
Size: 951 456
PasI CC'CwG_GG
Cuts at: 0 474 1407
Size: 474 933
PflMI CCAn_nnn'nTGG
Cuts at: 0 740 1407
Size: 740 667
PpuMI rG'GwC_Cy
Cuts at: 0 1092 1109 1407
Size: 1092 17 298
Fragments arranged by size:
1092 298 17
PspOMI G'GGCC_C
Cuts at: 0 160 819 1407
Size: 160 659 588
Fragments arranged by size:
659 588 160
PstI C_TGCA'G
Cuts at: 0 828 1407
Size: 828 579
PvuII CAG'CTG
Cuts at: 0 147 200 1311 1407
Size: 147 53 1111 96
Fragments arranged by size:
1111 147 96 53
SacII CC_GC'GG
Cuts at: 0 1379 1407
Size: 1379 28
SapI GCTCTTCn'nnn_
Cuts at: 0 1372 1407
Size: 1372 35
SbfI CC_TGCA'GG
Cuts at: 0 828 1407
Size: 828 579
SexAI A'CCwGG_T
Cuts at: 0 773 1407
Size: 773 634
SfcI C'TryA_G
Cuts at: 0 824 1407
Size: 824 583
SphI G_CATG'C
Cuts at: 0 951 1407
Size: 951 456
StyI C'CwwG_G
Cuts at: 0 1136 1407
Size: 1136 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 282 418 1197 1407
Size: 39 243 136 779 210
Fragments arranged by size:
779 243 210 136 39
Tth111I GACn'n_nGTC
Cuts at: 0 775 1407
Size: 775 632
XbaI T'CTAG_A
Cuts at: 0 861 1407
Size: 861 546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 1407
Size: 423 984
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI
AvaI BanI BanII BbsI BbvCI BglI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI
BsgI Bsp1286I BspHI BspMI BsrFI BssSI BstEII BstXI
BtgI BtgZI CspCI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I HincII KpnI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacII SapI SbfI SexAI SfcI
SphI StyI TaqII Tth111I XbaI XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BpuEI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstYI BstZ17I Bsu36I BtsI ClaI DraI DraIII
DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TatI TstI XhoI XmaI XmnI ZraI